Development of simple and accurate quantification method of waterborne pathogenic microorganisms using ABC-PCR
| Project/Area Number |
21860094
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | National Institute of Public Health |
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Principal Investigator |
KISHIDA Naohiro 国立保健医療科学院, 生活環境研究部, 主任研究官 (10533359)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,678,000 (Direct Cost: ¥2,060,000、Indirect Cost: ¥618,000)
Fiscal Year 2010: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000)
Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
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| Keywords | 水資源 / 水循環 / 遺伝子 / 病原微生物 / クリプトスポリジウム / 土木環境システム / 微生物 |
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| Research Abstract |
We describe an assay for simple and accurate quantification of pathogenic microorganisms in water samples using a recently developed quantification method named alternately binding probe competitive PCR(ABC-PCR). Cryptosporidium spp. were selected as representative pathogenic microorganisms in this study. The standard curve of the ABC-PCR assay had a good fitting to a rectangular hyperbola. Concentrations of Cryptosporidium oocysts in real river water samples were successfully quantified by the ABC-PCR assay. The quantified values by the ABC-PCR assay very closely resemble those by the real-time-PCR assay.
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Report
(3 results)
Research Products
(8 results)
