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Efficient production of novel biosurfactant by yeast isolated from deep sea

Research Project

Project/Area Number 21860097
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

KONISHI Masaaki  Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, 研究員 (90533860)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,678,000 (Direct Cost: ¥2,060,000、Indirect Cost: ¥618,000)
Fiscal Year 2010: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000)
Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
Keywords深海 / 酵母 / バイオサーファクタント / マンノシルエリスリトールリピッド / シュードザイマ / シロウリガイ
Research Abstract

We describe detailed structure determination of biosurfactant produced by Pseudozyma hubeiensis SY62, which was newly isolated from Calyptogena soyoae (deep-sea cold-seep clam, "Shirouri-gai") at 1,156 m in Sagami bay. Results of nuclear magnetic resonance spectroscopies indicated the major product, namely MEL-C, as a promising BS. According to surface tension determination, the novel MEL-C showed larger critical micelle concentration (1.1×10-5 M) than conventional MEL-C which bound C10 and C12 acids (9.1×10-6 M). We demonstrated a highly efficient production of MELs in the improved medium by fed-batch cultivation. The final concentration of MELs reached 129±8.2 g/L for one week.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results)

  • [Journal Article] 深海酵母の利用-バイオサーファクタントの生産-2010

    • Author(s)
      小西正朗
    • Journal Title

      高圧力の科学と技術 第10巻4号

      Pages: 347-353

    • NAID

      10027681103

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosurfactant-producing yeast isolated from Calyptogena soyoae (Deep-Sea Cold-Seep Clam) in the deep sea2010

    • Author(s)
      Konishi M., Fukuoka T., Nagahama T., Morita T., Imura T., Kitamoto D, Hatada Y.
    • Journal Title

      Journal of Bioscience and Bioengineering 110(2)

      Pages: 169-175

    • NAID

      110007700565

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 深海酵母の利用-バイオサーファクタントの生産-2010

    • Author(s)
      小西正朗
    • Journal Title

      高圧力の科学と技術

      Volume: 10 Pages: 347-353

    • NAID

      10027681103

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosurfactant-producing yeast isolated from Calyptogena soyoae(Deep-Sea Cold-Seep Clam)in the deep sea2010

    • Author(s)
      小西正朗
    • Journal Title

      Journal of Bioscience and Bioengineering (印刷中)

    • NAID

      110007700565

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yeast extract stimulates production of glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma hubeiensis SY62

    • Author(s)
      Konishi M, Nagahama T., Fukuoka T., Morita T., Imura T., Kitamoto D., Hatada Y
    • Journal Title

      Journal of Bioscience and Bioengineering (in press)

    • NAID

      110008672819

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Yeast extract stimulates production of glycolipid biosurfactants, mannosylerythritol lipids, by Pseudoryma hubeiensis SY62

    • Author(s)
      小西正朗, 他6名
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 深海由来酵母Puseudozyma hubeiensis SY62株を用いたバイオサーファクタントの生産2010

    • Author(s)
      小西正朗, 福岡徳馬, 長濱統彦, 森田友岳, 井村知弘, 北本大, 秦田勇二
    • Organizer
      第10回極限環境生物学会
    • Place of Presentation
      京都大学宇治キャンパス,京都
    • Year and Date
      2010-11-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 深海由来酵母Puseudozyma hubeiensis SY62株を用いたバイオサーファクタントの生産2010

    • Author(s)
      小西正朗, 他6名
    • Organizer
      第10回極限環境生物学会
    • Place of Presentation
      京都大学宇治キャンパス, 京都
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 深海由来酵母Puseudozyma hubeiensis SY62株を用いたバイオサーファクタントの効率生産2010

    • Author(s)
      小西正朗, 福岡徳馬, 長濱統彦, 森田友岳, 井村知弘, 北本大, 秦田勇二
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      フェニックス・シーガイヤ・リゾート,宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 深海由来酵母Puseudoryma hubeiensis SY62株を用いたバイオサーファクタントの効率生産2010

    • Author(s)
      小西正朗, 他6名
    • Organizer
      第62回日本生物工学会大会(2010)
    • Place of Presentation
      フェニックス・シーガイヤ・リゾート, 宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 深海由来酵母Pseudozyma hubeiensis SY62株が生産するバイオサーファクタント2009

    • Author(s)
      小西正朗, 福岡徳馬, 長濱統彦, 森田友岳, 井村知弘, 北本大, 秦田勇二
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学東山キャンパス,名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 深海由来酵母 Pseudozyma hubeiensis SY62株が生産するバイオサーファクタント2009

    • Author(s)
      小西正朗
    • Organizer
      第61回 日本生物工学会大会
    • Place of Presentation
      名古屋市・名古屋大学東山キャンパス
    • Year and Date
      2009-09-23
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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