Molecular mechanism of the initiation of homologous recombination by meiotic cohesin

• Project/Area Number: 21870007
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: KUGOU Kazuto The University of Tokyo, 大学院・総合文化研究科, 特任研究員 (60554425)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,665,000 (Direct Cost: ¥2,050,000、Indirect Cost: ¥615,000); Fiscal Year 2010: ¥1,248,000 (Direct Cost: ¥960,000、Indirect Cost: ¥288,000); Fiscal Year 2009: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
• Keywords: ゲノム動態 / 遺伝 / マイクロアレイ

Research Abstract

Meiosis is a special type of cell division for sexual reproduction and produces gametes. Genetic recombination between homologous chromosomes is required for meiosis. A defect in the recombination results in declining biodiversity and disorders caused by chromosomal abnormality. Meiotic chromosomes interact with various proteins. The interaction dynamically alters chromosome structure. In this study, we showed how a chromosomal structural protein regulates initiation of meiotic recombination at a molecular level.

Research Products

• [Journal Article] Actin-related protein Arp6 influences H2A.Z-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores (2010)
  • Author(s): Takahito Yoshida, Kenji Shimada, Yukako Oma, Veronique Kalck, Kazumi Akimura, Angela Taddei, Hitoshi Iwahashi, Kazuto Kugou, Kunihiro Ohta, Susan M Gasser, Masahiko Harata
  • Journal Title: PLoS Genetics Vol.6
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp6 influences H2A.Z-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores. (2010)
  • Author(s): Takahito Yoshida
  • Journal Title: PLoS Genetics Volume: VOL.6
  • Peer Reviewed
• [Journal Article] Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes (2009)
  • Author(s): Kazuto Kugou, Tomoyuki Fukuda, Shintaro Yamada, Masaru Ito, Hiroyuki Sasanuma, Saori Mori, Yuki Katou, Takehiko Itoh, Kouji Matsumoto, Takehiko Shibata, Katsuhiko Shirahige, Kunihiro Ohta
  • Journal Title: Molecular Biology of the Cell Vol.20 Pages: 3064-3076
  • Peer Reviewed
• [Journal Article] Genome-wide high-resolution chromatin immunoprecipitation of meiotic chromosomal proteins in Saccharomyces cerevisiae (2009)
  • Author(s): Kazuto Kugou, Kunihiro Ohta
  • Journal Title: Methods in Molecular Biology Vol.557 Pages: 285-304
  • Peer Reviewed
• [Journal Article] Rec8 guides canonical Spoll distribution along yeast meiotic chromosomes (2009)
  • Author(s): 久郷和人
  • Journal Title: Molecular Biology of the Cell VOL.20 Pages: 3064-3076
  • Peer Reviewed
• [Journal Article] Genome-wide high-resolution chromatin immunoprecipitation of meiotic chromosomal proteins in Saccharomyces cerevisiae (2009)
  • Author(s): 久郷和人
  • Journal Title: Methods in molecular biology VOL.557 Pages: 285-304
  • Peer Reviewed
• [Presentation] Regulation of the localization of DSB proteins on chromosomes by Rec8 (2011)
  • Author(s): 久郷和人
  • Organizer: The 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: The Low Wood Hotel, UK
  • Year and Date: 2011-04-13
• [Presentation] 染色体ドメインによる減数分裂期相同組換え開始制御と染色体接着因子の関係 (2011)
  • Author(s): 伊藤将
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 山代温泉瑠璃光(金沢)
  • Year and Date: 2011-01-12
• [Presentation] Control of recombination-initiating DNA double strand breaks by cohesion and chromosomal axis proteins (2010)
  • Author(s): Kunihiro Ohta
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] 染色体接着因子Rec8と遺伝的組換え開始因子Spo11の相互作用 (2010)
  • Author(s): 伊藤将
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] 減数分裂期姉妹染色体接着因子と減数分裂期相同組換えの関係 (2010)
  • Author(s): 久郷和人
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] 減数分裂期相同組換え開始因子と姉妹染色体接着因子Rec8の関係 (2010)
  • Author(s): 久郷和人
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2010-09-21
• [Presentation] 減数分裂期のDSB形成におけるRec8の役割 (2010)
  • Author(s): 久郷和人
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: ラフォーレ修善寺(伊豆)
  • Year and Date: 2010-05-27
• [Presentation] Spo11の染色体への結合とDSB形成におけるRec8の必要性 (2009)
  • Author(s): 久郷和人
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-12
• [Presentation] Spollの染色体への結合とDSB形成におけるRec8の必要性 (2009)
  • Author(s): 久郷和人
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Regulation of localization of Spo11 on chromosomes by meiotic cohesin component Rec8 (2009)
  • Author(s): 久郷和人
  • Organizer: International symposium on "Chromosome cycle and genome dynamics"
  • Place of Presentation: Sunvalley Resort Nasu, Tochigi, Tochigi, Japan
  • Year and Date: 2009-11-10
• [Presentation] Regulation of localization of Spoll on chromosomes by meiotic cohesin component Rec8 (2009)
  • Author(s): 久郷和人
  • Organizer: International symposium on"Chromosome cycle and genome dynamics"
  • Place of Presentation: Sunvalley Resort Nasu, Tochigi, Japan
  • Year and Date: 2009-11-10