| Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
|
|---|
| Research Abstract |
In this study, (1) more than 200-fold sensitivity enhancement was established for solid-state NMR spectroscopy using Dynamic Nuclear Polarization (DNP). (2) Application of the sparse data sampling technique enabled to detect conventionally unobservable signals. (3) A use of reserve isotope labeling technique resolved many previously unseen signals. (4) A new scheme to detect the molecular interfaces within a fibril was developed for high-selectivity and sensitivity. (5) ss2m was suggested to largely retain its native fold in the fibril, contrary to the widespread belief that amyloid fibril formation generally requires native proteins to adopt a fibril-specific fold.
|
