| Project/Area Number |
21870021
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
SATOUH Yuhkoh Osaka University, 免疫学フロンティア研究センター, 特任助教 (40545571)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
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| Keywords | 遺伝子操作動物 / 実験動物 / 受精 / 微絨毛 / 膜融合 |
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| Research Abstract |
In this study, I focus on the function of CD9 molecule and egg microvilli in mammalian fertilization. For that, I set two goals as 1) Analysis of fertilization ability of microvilli less mutant egg, and 2) Visualization of egg microvilli by using novel non-invasive imaging method. As a result until 2011, 1) I have obtained the data which strongly suggesting that the eggs without two of essential genes for constructing microvilli don't have fertilizing ability in vivo. And 2) I have established the imaging system which enable us to visualize egg microvilli in living form. By using the imaging system, I additively succeeded in visualizing the process of sperm-egg fusion in real-time scale for the first time.
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