Analysis of the activation mechanism of FilGAP by phosphorylation in establishment and maintenance of cell polarity.

Research Project

Project/Area Number	21870031
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Functional biochemistry
Research Institution	Kitasato University
Principal Investigator	NAKAZAWA Yuki Kitasato University, 理学部, 助教 (50508851)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000) Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000) Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywords	細胞極性 / リン酸化 / シグナル伝達
Research Abstract	To understand the activation mechanism of the Rac GAP FilGAP, several FilGAP-binding proteins were isolated and identified by mass-spectrometry. Theanalysis demonstrated that FilGAP forms complexes with several RNA binding proteins. Oneof them, RBM10 was found to bind to FilGAP at cell edges and its tyrosine phosphorylationby Src-family enhances their binding. It appeared that RBM10 regulates expression ofFilGAP. In addition, analysis using an antibody against phosphorylated(activated) FilGAPdemonstrated the activation level of FilGAP during cell spreading.

Report

(3 results)