Elucidation of the functional mechanism of the novel 2-component K^+/H^+ antiporters found in Escherichia coli and Bacillus subtilis.

• Project/Area Number: 21870035
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Toyo University
• Principal Investigator: FUJISAWA Makoto Toyo University, 生命科学部, 助教 (70549641)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: カリウムイオン / アンチポーター / 大腸菌 / 枯草菌 / 分子生物学

Research Abstract

To elucidate the functional mechanism of the novel 2-component K^+/H^+ antiporters composed from a membrane protein and a peripheral protein found in Escherichia coli and Bacillus subtilis, the detection systems of its interaction was established. Peptide tags were introduced into the proteins and the interaction between the proteins from B. subtilis was successfully detected by both pull-down assay and blue-native PAGE.

Research Products

• [Presentation] Detection of the interaction between membrane protein YhaU and hydrophilic protein YhaT of the 2-component K^+/H^+ antiporter from Bacillus subtilis. (2010)
  • Author(s): 藤澤誠
  • Organizer: 8^<th> International Symposium on Biocsience and Nanotechnology.
  • Place of Presentation: 東洋大学(東京都)
• [Presentation] CPA2ファミリーに属する2成分K+/H+アンチポーターのサブユニット間の相互作用の検出 (2010)
  • Author(s): 藤澤誠
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Detection of the interaction between membrane protein YhaU and hydrophilic protein YhaT of the 2-component K^+/H^+ antiporter from Bacillus subtilis. (2010)
  • Author(s): Makoto Fujisawa
  • Organizer: 8^<th> International Symposium on Bioscience and Nanotechnology.
  • Place of Presentation: 東洋大学白山キャンパス(東京都)
• [Presentation] CPA2ファミリーに属する2成分K^+/H^+アンチポーターのサブユニット間の相互作用の検出 (2010)
  • Author(s): 藤澤誠
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)