Elucidation of the functional mechanism of the novel 2-component K^+/H^+ antiporters found in Escherichia coli and Bacillus subtilis.
| Project/Area Number |
21870035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Toyo University |
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Principal Investigator |
FUJISAWA Makoto Toyo University, 生命科学部, 助教 (70549641)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
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| Keywords | カリウムイオン / アンチポーター / 大腸菌 / 枯草菌 / 分子生物学 |
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| Research Abstract |
To elucidate the functional mechanism of the novel 2-component K^+/H^+ antiporters composed from a membrane protein and a peripheral protein found in Escherichia coli and Bacillus subtilis, the detection systems of its interaction was established. Peptide tags were introduced into the proteins and the interaction between the proteins from B. subtilis was successfully detected by both pull-down assay and blue-native PAGE.
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Report
(3 results)
Research Products
(4 results)
