Labeling of torque generation site of bacterial flagellar motors

• Project/Area Number: 21870036
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Hosei University
• Principal Investigator: SOWA Yoshiyuki 法政大学, 生命科学部, 講師 (10519440)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: べん毛モーター / ナノマシン / 細菌べん毛モーター / 1分子計測 / 生体ナノマシン / 蛍光観察

Research Abstract

The bacterial flagellar motor is a rotary nano-machine of～ 50 nm in diameter. This rotary motor possesses many insights to build artificial nano-machines, e. g. fast rotation, switching of motor direction and so on. To reveal its rotation mechanism, we have tried to develop a method to observe the torque generation sites of the motor. As a result, we succeeded in labeling the functional motor by fluorescent dyes.

Research Products

• [Journal Article] A simple back-scattering microscope for fast tracking of biological molecules (2010)
  • Author(s): Sowa, Y., Steel, B. C. & Berry, R. M.
  • Journal Title: Rev. Sci. Instrum. Volume: 81 Pages: 113704-113704
  • Peer Reviewed
• [Journal Article] A simple back-scattering microscope for fast tracking of biological molecules (2010)
  • Author(s): Sowa, Y.
  • Journal Title: Rev.Sci.Instrnm. Volume: 81 Pages: 113704-6
  • Peer Reviewed
• [Journal Article] Steps in the bacterial flagellar motor (2009)
  • Author(s): Mora, T., Yu, H., Sowa, Y. & Wingreen, N.
  • Journal Title: PLoS Comput. Biol Volume: 5
  • NAID: 110004865189
  • Peer Reviewed
• [Journal Article] Steps in the bacterial flagellar motor (2009)
  • Author(s): Mora, T.
  • Journal Title: PLoS Comput.Biol Vol.5
  • Peer Reviewed
• [Presentation] Single-molecule analysis of bacterial flagellar motor (2011)
  • Author(s): 曽和義幸
  • Organizer: The 1497th Biological Symposium
  • Place of Presentation: 遺伝研(静岡県)
  • Year and Date: 2011-03-29
• [Presentation] Single-molecule analysis of bacterial flagellar motor (2011)
  • Author(s): 曽和義幸
  • Organizer: The 1497th Biological Symposium
  • Place of Presentation: 遺伝研,静岡
  • Year and Date: 2011-03-29
• [Presentation] Discrete steps in fast bacterial flagellar rotation detected by back-scattering microscopy (2010)
  • Author(s): 曽和義幸
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-22
• [Presentation] Discrete steps in fast bacterial flagellar rotation detected by back-scattering microscopy (2010)
  • Author(s): 曽和義幸
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 東北大学,宮城
  • Year and Date: 2010-09-22
• [Presentation] 細菌べん毛モーターの動きを観る (2010)
  • Author(s): 曽和義幸
  • Organizer: 第4回細菌学・若手コロッセウム
  • Place of Presentation: ラフォーレ修善寺(静岡県)
  • Year and Date: 2010-08-28
• [Presentation] 細菌べん毛モーターの動きを観る (2010)
  • Author(s): 曽和義幸
  • Organizer: 第4回細菌学・若手コロッセウム
  • Place of Presentation: ラフォーレ修善寺,静岡
  • Year and Date: 2010-08-28
• [Presentation] 誘引物質に対する大腸菌応答のビデオ解析 (2010)
  • Author(s): 曽和義幸
  • Organizer: 微生物研究会
  • Place of Presentation: 法政大学(東京都)
  • Year and Date: 2010-06-26
• [Presentation] 誘引物質に対する大腸菌応答のビデオ解析 (2010)
  • Author(s): 曽和義幸
  • Organizer: 微生物研究会
  • Place of Presentation: 法政大学,東京
  • Year and Date: 2010-06-26
• [Presentation] 誘引物質に対する大腸菌の応答・適応過程のビデオ解析 (2010)
  • Author(s): 曽和義幸
  • Organizer: 2009年度べん毛研究交流会
  • Place of Presentation: 松風園(愛知県)
  • Year and Date: 2010-03-15
• [Presentation] 誘引物質に対する大腸菌の応答・適応過程のビデオ解析 (2010)
  • Author(s): 曽和義幸
  • Organizer: 2009年度べん毛研究交流会
  • Place of Presentation: (松風園)愛知県
  • Year and Date: 2010-03-15
• [Presentation] Steps in fast flagellar rotation (2009)
  • Author(s): 曽和義幸
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島県)
  • Year and Date: 2009-11-01
• [Presentation] Single-molecule analysis of bacterial flagellar motility (2009)
  • Author(s): 曽和義幸
  • Organizer: 第19回 日本数理生物学会年会
  • Place of Presentation: 東京大学駒場キャンパス(東京都)
  • Year and Date: 2009-09-11
• [Presentation] モーター回転を高速度で追跡する (2009)
  • Author(s): 曽和義幸
  • Organizer: 理研シンポジウム
  • Place of Presentation: 理化学研究所(埼玉県)
  • Year and Date: 2009-04-09
• [Book] Handbook of Single-Molecule Biophysics (2009)
  • Author(s): Pilizota, T., Sowa Y. & Berry RM.
  • Publisher: Springer
• [Book] Handbook of Single-Molecule Biophysics(分担執筆) (2009)
  • Author(s): Pilizota, T.
  • Total Pages: 648
  • Publisher: Springer
• [Remarks]
  • URL: http://sowalab.ws.hosei.ac.jp/index.html