Functional analysis of a novel RAD51-binding protein, EVL in the homologous recombinational repair

• Project/Area Number: 21870037
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Waseda University
• Principal Investigator: TAKAKU Motoki Waseda University, 理工学術院, 助手 (30547071)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: ゲノム構築・機能・維持 / タンパク質と酵素 / 核酸 / 遺伝子及び染色体

Research Abstract

EVL promotes ssDNA catenation in the presence of human TOPOIIIα, which is a type I topoisomerase. Therefore EVL and TOPOIIIα may function in the processing of DNA intermediates formed during homologous recombinational repair (HRR). C-terminal EVH2 domain of EVL is responsible for the recombination activity of EVL. RAD51, RPA and TOPOIIIα promote recombination reaction between circular ssDNA and supercoiled dsDNA. It suggests that these proteins cooperatively function in the initial step of HRR.

Research Products

• [Journal Article] Biochemical analysis of the human Ena/Vasp- family proteins, MENA, VASP, and EVL, in homologous recombination (2011)
  • Author(s): Motoki Takaku , Hiroyuki Ueno, Hitoshi Kurumizaka
  • Journal Title: The Journal of Biochemistry (in press)
  • NAID: 10030585933
  • Peer Reviewed
• [Journal Article] Halenaquinone a chemical compound that specifically inhibits the secondary DNA binding of RAD51 (2011)
  • Author(s): Motoki Takaku, Takashi Kainuma, Takako Ishida-Takaku, Shintaro Ishigami, Hidekazu Suzuki, Satoshi Tashiro, Rob W.M.van Soest, Yoichi Nakao, Hitoshi Kurumizaka
  • Journal Title: Genes to Cells Vol.16 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Halenaquinone a chemical compound that specifically inhibits the secondary DNA binding of RAD51 (2011)
  • Author(s): Motoki Takaku, Takashi Kainuma, Takako Ishida-Takaku, et al.
  • Journal Title: Genes to Cells Volume: 16 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Single-stranded DNA catenation mediated by human EVL and a type I topoisomerase (2010)
  • Author(s): Motoki Takaku, Daisuke Takahashi, Shinichi Machida, Hiroyuki Ueno, Noriko Hosoya, Shukuko Ikawa, Kiyoshi Miyagawa, Takehiko Shibata, Hitoshi Kurumizaka
  • Journal Title: Nucleic Acids Research Vol.38 Pages: 3959-68
  • Peer Reviewed
• [Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination (2010)
  • Author(s): Yoshimasa Takizawa, Yong Qing, Motoki Takaku, Takako Ishida, Yuichi Morozumi, Takashi Tsujita, Toshiaki Kogame, Kouji Hirota, Masayuki Takahashi, Takehiko Shibata, Hitoshi Kurumizaka, Shunichi Takeda
  • Journal Title: Nucleic Acids Research Vol.38 Pages: 5059-74
  • Peer Reviewed
• [Journal Article] Holliday junction binding activity of human SPF45 (2010)
  • Author(s): Naoki Horikoshi, Yuichi Morozumi, Motoki Takaku, Yoshimasa Takizawa, Hitoshi Kurumizaka
  • Journal Title: Genes to Cells Vol.15 Pages: 373-383
  • Peer Reviewed
• [Journal Article] Single-stranded DNA catenation mediated by human EVL and a type I topoisomerase (2010)
  • Author(s): Motoki Takaku, Daisuke Takahashi, Shinichi Machida., et al.
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 3959-3968
  • Peer Reviewed
• [Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination (2010)
  • Author(s): Yoshimasa Takizawa, Yong Qing, Motoki Takaku, et al.
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 5059-5074
  • Peer Reviewed
• [Journal Article] Holliday junction binding activity of human SPF45 (2010)
  • Author(s): Naoki Horikoshi, Yuichi Morozumi, Motoki Takaku, et al.
  • Journal Title: Genes to Cells Volume: 15 Pages: 373-383
  • Peer Reviewed
• [Journal Article] Biochemical analysis of the human EVL domains in homologous recombination (2009)
  • Author(s): Motoki Takaku, Shinichi Machida, Shugo Nakayama, Daisuke Takahashi, Hitoshi Kurumizaka
  • Journal Title: The FEBS journal Vol.276 Pages: 5841-5848
  • Peer Reviewed
• [Journal Article] Human PSF binds to RAD51 and modulates its homologous-pairing and strandexchange activities (2009)
  • Author(s): Yuichi Morozumi, Yoshimasa Takizawa, Motoki Takaku, Hitoshi Kurumizaka
  • Journal Title: Nucleic Acids Research Vol.37 Pages: 4296-307
  • Peer Reviewed
• [Journal Article] The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction (2009)
  • Author(s): Mari Katsura, Takanori Tsuruga, Osamu Date, Takashi Yoshihara, Mari Ishida, Yoshitaka Tomoda, Miyuki Okajima, Motoki Takaku, Hitoshi Kurumizaka, Aiko Kinomura, Hiromu K. Mishima, Kiyoshi Miyagawa
  • Journal Title: Nucleic Acids Research Vol.37 Pages: 3959-68
  • Peer Reviewed
• [Journal Article] Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL (2009)
  • Author(s): Motoki Takaku, Shinichi Machida, Noriko Hosoya, Shugo Nakayama, Yoshimasa Takizawa, Isao Sakane, Takehiko Shibata, Kiyoshi Miyagawa, Hitoshi Kurumizaka
  • Journal Title: The Journal of Biological Chemistry Vol.284 Pages: 14326-14336
  • Peer Reviewed
• [Journal Article] Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL (2009)
  • Author(s): Motoki Takaku, Shinichi Machida, Noriko Hosoya, …, Hitoshi Kurumizaka
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 14326-14336
  • Peer Reviewed
• [Journal Article] The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction (2009)
  • Author(s): Mari Katsura, …, Motoki Takaku, …, Kiyoshi Miyagawa
  • Journal Title: Nucleic Acids Research 37 Pages: 3959-3968
  • Peer Reviewed
• [Journal Article] Human PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities (2009)
  • Author(s): Yuichi Morozumi, Yoshimasa Takizawa, Motoki Takaku, Hitoshi Kurumizaka
  • Journal Title: Nucleic Acids Research 37 Pages: 4296-4307
  • Peer Reviewed
• [Journal Article] Biochemical analysis of the human EVL domains in homologous recombination (2009)
  • Author(s): Motoki Takaku, Shinichi Machida, …, Hitoshi Kurumizaka
  • Journal Title: The FEBS journal 276 Pages: 5841-5848
  • Peer Reviewed
• [Presentation] A novel homologous recombination factor, human EVL forms single-stranded DNA catemers with a type I topoisomerase (2011)
  • Author(s): Motoki Takaku, Daisuke Takahashi, Shinichi Machida, Hiroyuki Ueno, Hitoshi Kurumizaka
  • Organizer: International Symposium on Physicochemical Field for Genetic Activities
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2011-01-24
• [Presentation] RAD51によるparanemic joint molecule形成過程へのtopoisomerase IIIαの役割 (2010)
  • Author(s): 高久誉大, 高橋大介, 胡桃坂仁志
  • Organizer: 第33回 日本分子生物学会年会 第83回 日本生化学会大会 合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] 新規RAD51組換え活性制御化合物の同定とその作用機序 (2010)
  • Author(s): 石田恭子, 海沼嵩, 高久誉大, 鈴木秀和, 田代聡, 石上進太郎, 他
  • Organizer: 第33回 日本分子生物学会年会 第83回 日本生化学会大会 合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] PSFによるDNA組換え酵素DMC1の活性化 (2010)
  • Author(s): 両角佑一, 高久誉大, 上野裕之, 胡桃坂仁志
  • Organizer: 第33回 日本分子生物学会年会 第83回 日本生化学会大会 合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] 新規RAD51活性化因子GEMIN2の相同組換え修復における機能 (2010)
  • Author(s): 高久誉大, 滝沢由政, 辻田幹, 石田恭子, 両角佑一, 胡桃坂仁志
  • Organizer: 第9回 核ダイナミクス研究会
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 2010-05-27
• [Presentation] RAD51によるparanemic joint molecule形成過程へのtopoisomerase IIIαの役割 (2010)
  • Author(s): 高久誉大、高橋大介、胡桃坂仁志
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] 新規RAD51活性化因子GEMIN2の相同組換え修復における機能 (2010)
  • Author(s): 高久誉大, 滝沢由政, 辻田幹, 石田恭子, 両角佑一, 胡桃坂仁志
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 静岡県伊豆市
• [Presentation] RAD51と相互作用するSMN-GEMIN2複合体の生化学的解析 (2009)
  • Author(s): 辻田幹, 高久誉大, 滝沢由政, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県 横浜市
  • Year and Date: 2009-12-12
• [Presentation] 新規相同組換え修復タンパク質ヒトSPF45の生化学的解析 (2009)
  • Author(s): 堀越直樹, 両角佑一, 高久誉大, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県 横浜市
  • Year and Date: 2009-12-12
• [Presentation] GEMIN2によるRAD51組換え活性の制御 (2009)
  • Author(s): 高久誉大, 滝沢由政, 辻田幹, 石田恭子, 両角佑一, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県 横浜市
  • Year and Date: 2009-12-11
• [Presentation] ヒト相同組換えタンパク質DMC1と相互作用する新規タンパク質PLEKHF1の生化学的機能解析 (2009)
  • Author(s): 岩下紗美香, 高久誉大, 引場樹里, 滝沢由政, 小布施力史, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県 横浜市
  • Year and Date: 2009-12-11
• [Presentation] ヒトRAD51と相互作用する新規タンパク質PSFの機能解析 (2009)
  • Author(s): 両角佑一, 滝沢由政, 富久誉大, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県 横浜市
  • Year and Date: 2009-12-11
• [Presentation] GEMIN2によるRAD51組換え活性の制御 (2009)
  • Author(s): 高久誉大, 滝沢由政, 辻田幹, 石田恭子, 両角佑一, 胡桃坂仁志
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 新規RAD51結合タンパク質EVLの機能解析 (2009)
  • Author(s): 高久誉大, 町田晋一, 細谷紀子, 宮川清, 胡桃坂仁志
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市