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The analysis of the regulation mechanisms of plant immune receptors

Research Project

Project/Area Number 21870044
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KADOTA Yasuhiro  The Institute of Physical and Chemical Research, 植物免疫研究グループ, 基礎科学特別研究員 (80548975)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywords植物免疫 / 分子シャペロン / 抵抗性蛋白質 / 蛋白質立体構造解析 / 病害抵抗性
Research Abstract

Hsp90-mediated function of nucleotide-binding leucine-rich repeat receptors (NLR protein) in plant and animal innate immunity depends on the cochaperone Sgt1 and, at least in plants, on Rar1. Both RAR1 and SGT1 domains are independently capable of interacting with the molecular chaperone Hsp90 and can coexist in complexes with Hsp90. We have determined the core structure of an Hsp90-SGT1-RAR1 ternary complex. Mutational and biochemical analyses define the architecture of the ternary complex that recruits NLR proteins by manipulating the structural elements to control the ATPase-dependent conformational cycle of the chaperone.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (11 results) Remarks (8 results)

  • [Journal Article] 動植物共通の免疫センサーの制御に働くタンパク質複合体2011

    • Author(s)
      門田康弘,白須賢
    • Journal Title

      化学と生物 49

      Pages: 154-156

    • Related Report
      2010 Final Research Report
  • [Journal Article] 動植物共通の免疫センサーの制御に働くタンパク質複合体2011

    • Author(s)
      門田康弘
    • Journal Title

      化学と生物

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Negative feedback regulation of microbe-associated molecular pattern-induced cytosolic Ca(2+) transients by protein phosphorylation.2011

    • Author(s)
      来須孝光
    • Journal Title

      Journal of Plant Research

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation-dependent differential regulation of plant growth, cell death and innate immunity by the regulatory receptor-like kinase BAK12011

    • Author(s)
      Ben Schwessinger
    • Journal Title

      PLoS Genetics

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for assembly of Hsp90-Sgt1-CHORD protein complexes: implications for chaperoning of NLR innate immunity receptors.2010

    • Author(s)
      Zhang M, Kadota Y, Prodromou C, Shirasu K, Pearl LH.
    • Journal Title

      Mol Cell 39

      Pages: 269-81

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NLR sensors meet at the SGT1-HSP90 crossroad.2010

    • Author(s)
      Kadota Y, Shirasu K, Guerois R.
    • Journal Title

      Trends Biochem Sci. 35

      Pages: 199-207

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NLR sensors meet at the SGT1-HSP90 crossroad.2010

    • Author(s)
      門田康弘
    • Journal Title

      Trends in Biochemical Sciences

      Volume: 35 Pages: 199-207

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for assembly of Hsp90-Sgt1-CHORD protein complexes : implications for chaperoning of NLR innate immunity receptors.2010

    • Author(s)
      Minghao Zhang, 門田康弘
    • Journal Title

      Molecular Cell

      Volume: 39 Pages: 269-281

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NLR sensors meet at the SGTI-HSP90 crossroad2010

    • Author(s)
      門田康弘
    • Journal Title

      Trends in Biochemical Sciences (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation-dependent differential regulation of plant growth, cell death and innate immunity by the regulatory receptor-like kinase BAK1.

    • Author(s)
      Schwessinger B, Roux M, Kadota Y, Ntoukakis V, Sklenar J, Jones A, Zipfel C
    • Journal Title

      PLoS Genetics (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells.

    • Author(s)
      Ohno R, Kadota Y, Fujii S, Sekine M, Umeda M, Kuchitsu K
    • Journal Title

      Plant Cell Physiol (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Negative feedback regulation of microbe-associated molecular pattern-induced cytosolic Ca^<2+> transients by protein phosphorylation.

    • Author(s)
      Kurusu T, Hamada H, Sugiyama Y, Yagala T, Kadota Y, Furuichi T, Hayashi T, Umemura K, Komatsu S, Miyao A, Hirochika H, Kuchitsu K
    • Journal Title

      Journal of Plant Research. (in press)

    • NAID

      10028258282

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] ジャガイモ疫病菌エフェクターAVR3aの構造機能解析2011

    • Author(s)
      八丈野孝,門田康弘,瀧澤香,李華,大沢登,半田徳子,寺田貴帆,小柴生造,白水美香子,渡部暁,木川隆則,横山茂之,Sophien Kamoun,白須賢
    • Organizer
      日本植物病理学会2010年度大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] ジャガイモ疫病菌エフェクターAVR3aの立体構造および機能解析2011

    • Author(s)
      八丈野孝
    • Organizer
      日本植物病理学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ジャガイモ疫病菌エフェクターAVR3aの立体構造および機能解析」2011

    • Author(s)
      八丈野孝,門田康弘,瀧澤香,李華,大沢登,半田徳子,寺田貴帆,小柴生造,白水美香子,渡部暁,木川隆則,横山茂之,Sophien Kamoun,白須賢
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] ジャガイモ疫病菌エフェクターAVR3aの構造機能解析2011

    • Author(s)
      八丈野孝
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 抵抗性蛋白質の安定化に必須なRAR1-SGT1-HSP90複合体の立体構造及び機能解析2010

    • Author(s)
      門田康弘,Minghao Zhang,竹林有理佳,Chrisostomos Prodromou, Raphael Guerois, Laurence H.Pearl,白須賢
    • Organizer
      日本植物病理学会2009年度大会
    • Place of Presentation
      国立京都国際会館(口頭発表)
    • Year and Date
      2010-04-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 動植物共通の免疫レセプターの安定化に必須なRAR1-SGT1-HSP90複合体の立体構造及び機能解析2010

    • Author(s)
      門田康弘,Zhang M.,竹林有理佳,Prodromou C., Guerois R., Pearl H.,白須賢
    • Organizer
      日本植物生理学会2009年度大会
    • Place of Presentation
      熊本大学(口頭発表)
    • Year and Date
      2010-03-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 動植物共通の免疫レセプターの安定化に必須なRAR1-SGT1-HSP90複合体の立体構造及び機能解析2010

    • Author(s)
      門田康弘
    • Organizer
      第51回 日本植物生理学会年会
    • Place of Presentation
      熊本県、熊本市
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural and Functional Analysis of the Chaperone Complex Required for NLR-type Immune Receptors2009

    • Author(s)
      Shirasu K, Kadota Y, Zhang M, Guerois R, Prodromou C, Pearl L
    • Organizer
      3rd Asia-Pacific International Peptide Symposium 2009
    • Place of Presentation
      Jeju Island Korea
    • Year and Date
      2009-11-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Structural and Functional Analysis of the Chaperone Complex Required for NLR-type Immune Receptors2009

    • Author(s)
      白須賢
    • Organizer
      3rd Asia-Pacific International Peptide Symposium
    • Place of Presentation
      Jeju Island, Korea
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural and functional analysis of the SGT1-RAR1-HSP90 complex required for plant immunity2009

    • Author(s)
      Kadota Y, Zhang M, Guerois R, Prodromou C, Pearl LH, Shirasu K
    • Organizer
      The 14th Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Quebec, Canada
    • Year and Date
      2009-07-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Structural and functional analysis of the SGT1-RARI-HSP90 complex required for plant immunity2009

    • Author(s)
      白須賢
    • Organizer
      IS-MPMI 2009 XIV International Congress
    • Place of Presentation
      Quebec, Canada
    • Year and Date
      2009-07-21
    • Related Report
      2009 Annual Research Report
  • [Remarks] 新聞報道:

    • Related Report
      2010 Final Research Report
  • [Remarks] 「ウィルスの侵入防止 植物のメカニズム解明」日刊工業新聞 2010年7月30日掲載

    • Related Report
      2010 Final Research Report
  • [Remarks] 「植物の免疫センサー 制御する複合体の構造」科学新聞 2010年8月6日掲載

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等:

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/r-world/info/release/press/2010/100730/detail.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/engn/r-world/info/release/press/2010/100730/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/r-world/info/release/press/2010/100730/detail.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/engn/r-world/info/release/press/2010/100730/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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