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Role of general stress sigma factor RpoS in Salmonella VBNC state

Research Project

Project/Area Number 21880005
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

KUSUMOTO Akiko  Obihiro University of Agriculture and Veterinary Medicine, 動物・食品衛生研究センター, 助教 (60535326)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
KeywordsVBNC / VNC / ストレス応答 / シグマ因子 / RpoS / サルモネラ / 細菌 / 食品 / ストレス / 発現制御 / 微生物
Research Abstract

General stress sigma factor RpoS delayed induction of viable but non-culturable (VBNC) state in Salmonella. Compared to S.Dublin harboring wild-type RpoS, S.Typhimurium strain LT2 and S.Oranienburg entered VBNC state rapidly due to lower levels of RpoS and missense mutation in rpoS gene, respectively.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (8 results) Book (2 results)

  • [Journal Article] Conversion of mono-polar to peritrichous flagellation in Vibrio alginolyticus.2011

    • Author(s)
      Kojima M, Nishioka N, Kusumoto A, Yagasaki J, Fukuda T, Homma M.
    • Journal Title

      Microbiology and Immunology. 55(2)

      Pages: 76-83

    • NAID

      10031121444

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conversion of mono-polar to peritrichous flagellation in Vibrio alginolyticus.2011

    • Author(s)
      Kojima M, Nishioka N, Kusumoto A, Yagasaki J, Fukuda T, Homma M.
    • Journal Title

      Microbiology and Immunology

      Volume: 55(2) Pages: 76-83

    • NAID

      10031121444

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of Listeria monocytogenes from commercial asazuke (Japanese light pickles).2010

    • Author(s)
      Maklon K, Minami A, Kusumoto A, Takeshi K, Nguyen TB, Makino S, Kawamoto K.
    • Journal Title

      International journal of food microbiology. 139(3)

      Pages: 134-139

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of Listeria monocytogenes from commercial asazuke(Japanese light pickles).2010

    • Author(s)
      Maklon K, Minami A, Kusumoto A, Takeshi K, Nguyen TB, Makino S, Kawamoto K.
    • Journal Title

      International journal of food microbiology

      Volume: 139(3) Pages: 134-139

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 食品における赤痢菌検出法の感度の向上2010

    • Author(s)
      門田修子、楠本晃子、牧野壮一、川本恵子
    • Journal Title

      日本獣医公衆衛生学会誌

      Volume: 63 Pages: 297-300

    • NAID

      10026516621

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of Listeria monocytogenes from commercial asazuke(Japanese light pickles).2010

    • Author(s)
      Maklon K, Minami A, Kusumoto A, Takeshi K, Nguyen TB, Makino S, Kawamoto K.
    • Journal Title

      International Journal of Food Microbiology 139巻

      Pages: 134-139

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 食品における赤痢菌検出法の感度の向上2010

    • Author(s)
      門田修子, 楠本晃子, 牧野壮一, 川本恵子
    • Journal Title

      日本獣医師公衆衛生学会誌 63巻

      Pages: 297-300

    • NAID

      10026516621

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salmonella Typhimurium isolated from healthy pigs and their ability of horizontal transfer of multidrug resistance and virulence genes.2009

    • Author(s)
      Nguyen Thi Bich Thuy, Koichi Takeshi, Akiko Kusumoto, Sou-ichi Makino, Keiko Kawamoto.
    • Journal Title

      Bioscience and Microflora. Vol.28 No.4

      Pages: 135-143

    • NAID

      10026974992

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutational analysis of the GTP-binding motif of F1hF which regulates the number and placement of the polar flagellum in Vibrio alginolyticus.2009

    • Author(s)
      Kusumoto A, Nishioka N, Koj ima S, Homma M.
    • Journal Title

      Journal of Biochemistry (Tokyo) 146(5)

      Pages: 643-650

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutational analysis of the GTP-binding motif of FlhF which regulates the number and placement of the polar flagellum in Vbrio alginolyticus2009

    • Author(s)
      Kusumoto A, Nishioka N, Kojima S, Homma M.
    • Journal Title

      Journal of Biochemistry(Tokyo) 146巻

      Pages: 643-650

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salmonella Typhimurium isolated from healthy pigs and their ability of horizontal transfer of multidrug resistance and virulence genes.2009

    • Author(s)
      Nguyen Thi Bich Thuy, Koichi Takeshi, 楠本晃子, 牧野壮一, 川本恵子
    • Journal Title

      Bioscience and microflora. 28巻

      Pages: 135-143

    • NAID

      10026974992

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ストレス応答関連シグマ因子RpoSはサルモネラのVBNC状態への移行を遅らせる2011

    • Author(s)
      楠本晃子、原田俊彦、牧野壮一、川本恵子
    • Organizer
      第151回日本獣医学会学術集会
    • Place of Presentation
      東京農工大大会中止のため要旨集による発表
    • Related Report
      2010 Final Research Report
  • [Presentation] ストレス応答関連シグマ因子RpoSはサルモネラのVBNC状態への移行を遅らせる2011

    • Author(s)
      楠本晃子、原田俊彦、牧野壮一、川本恵子
    • Organizer
      第151回日本獣医学会学術集会
    • Place of Presentation
      東京農工大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] サルモネラVBNCにおけるストレス応答関連シグマ因子RpoSの役害2010

    • Author(s)
      楠本晃子、牧野壮一、川本恵子
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Related Report
      2010 Final Research Report
  • [Presentation] サルモネラの生きているが培養できない(VBNC)状態におけるストレス応答関連シグマ因子RpoSの役割2010

    • Author(s)
      楠本晃子、牧野壮一、川本恵子
    • Organizer
      第78回日本細菌学会北海道支部・第28回日本クラミジア研究会合同学術集会
    • Place of Presentation
      北海道大学および酪農学園大学
    • Related Report
      2010 Final Research Report
  • [Presentation] サルモネラの生きているが培養できない状態への移行におけるストレス応答関連シグマ因子RpoSの役割2010

    • Author(s)
      楠本晃子、牧野壮一、川本恵子
    • Organizer
      第7回21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア阿蘇
    • Related Report
      2010 Final Research Report
  • [Presentation] サルモネラVBNCにおけるストレス応答関連シグマ因子RpoSの役割2010

    • Author(s)
      楠本晃子、牧野壮一、川本恵子
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] サルモネラの生きているが培養できない(VBNC)状態におけるストレス応答関連シグマ因子RpoSの役割2010

    • Author(s)
      楠本晃子、牧野壮一、川本恵子
    • Organizer
      第78回日本細菌学会北海道支部・第28回日本クラミジア研究会合同学術集会
    • Place of Presentation
      北海道大学、酪農学園大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] 赤痢菌におけるH-NSが制御するVBNC関運遺伝子の解析2010

    • Author(s)
      楠本晃子、牧野壮一、門田修子、川本恵子
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Book] 獣医微生物学実験マニュアル(第3章 固形培地とコロニー観察)2009

    • Author(s)
      牧野壮一、楠本晃子
    • Publisher
      チクサン出版社
    • Related Report
      2010 Final Research Report
  • [Book] 獣医微生物学実験マニュアル 第3章 固形培地とコロニー観察2009

    • Author(s)
      牧野壮一/楠本晃子
    • Total Pages
      216
    • Publisher
      チクサン出版社
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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