Serological characterizations of tandem repeat proteins for detection of African trypanosome infection
| Project/Area Number |
21880007
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo (2010)
Obihiro University of Agriculture and Veterinary Medicine (2009) |
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Principal Investigator |
GOTO Yasuyuki The University of Tokyo, 大学院・農学生命科学研究科, 助教 (50553434)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000)
Fiscal Year 2010: ¥1,131,000 (Direct Cost: ¥870,000、Indirect Cost: ¥261,000)
Fiscal Year 2009: ¥1,495,000 (Direct Cost: ¥1,150,000、Indirect Cost: ¥345,000)
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| Keywords | トリパノソーマ症 / 血清学的診断 / 抗原 / タンデムリピート |
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| Research Abstract |
Serological diagnosis is a useful method to detect African trypanosome infection. Currently available serological tests utilize whole parasites or crude antigens. Recombinant antigens may not only enhance test specificity by allowing the detection of species-specific antibodies, but could also improve reproducibility/standardization and reduce production costs. With a goal of identifying such recombinant proteins, we computationally identified proteins with tandem repeat (TR) domain from the parasite proteomes, expressed them and evaluated their potential for serological diagnosis of African trypanosome infections in cattle. Among those tested, Tbg4 demonstrated the best performance with 89% sensitivity and 100% specificity. Our data indicate the potential of these defined antigens for detection of animal African trypanosomiasis.
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Report
(3 results)
Research Products
(7 results)
