Molecular basis of oil-degrading methanogenic microbial symbiotic system indigenous to petroleum reservoir

• Project/Area Number: 21880014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: KOBAYASHI Hajime 東京大学, 大学院・工学系研究科, 助教 (50549269)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: 地下微生物 / 油田 / メタン菌 / 石油 / 共生 / メタン / 原油分解 / 環境微生物 / 分子系統解析 / 酢酸資化性メタン菌

Research Abstract

To gain basic knowledge for industrial application of crude-oil-degrading methanogenic microorganisms endogenous to petroleum reservoir, we collected reservoir brine samples from petroleum reservoirs and analyzed population structure and methanogenic activity of microorganisms in the reservoirs. Our results suggested an important role of acetoclastic methanogens in the microbial system within a biodegraded oil reservoir. Moreover, we showed that supplementation of complex nutrients could enhance the methanogenic activity of microorganisms endogenous to a depleted oil reservoir.

Research Products

• [Journal Article] Phylogenetic diversity of microbial communities associated with the crude oil and formation water phases of the produced fluid from a high-temperature biodegraded petroleum reservoir. (2012)
  • Author(s): H. Kobayashi, K. Endo, S. Sakata, D. Mayumi, H. Kawaguchi, M. Ikarashi, Y. Miyagawa, H. Maeda and K. Sato
  • Journal Title: J Biosci Bioeng Volume: 113 Issue: 2 Pages: 204-10
  • DOI: 10.1016/j.jbiosc.2011.09.015
  • Peer Reviewed
• [Journal Article] Analysis of methane production by microorganisms indigenous to a depleted oil reservoir for application in Micobial Enchance Oil Recoverly. (2012)
  • Author(s): H. Kobayashi, H. Kawaguchi, K. Endo, D. Mayumi, S. Sakata, M. Ikarashi, Y. Miyagawa, H. Maeda and K. Sato
  • Journal Title: J Biosci Bioeng Volume: 113 Issue: 1 Pages: 84-7
  • DOI: 10.1016/j.jbiosc.2011.09.003
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle and formation-water components of the reservoir fluid from a non-flooded high-temperature petroleum reservoir (2012)
  • Author(s): 小林肇、遠藤啓太、坂田将、眞弓大介、川口秀夫、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 113 Pages: 360-366
  • NAID: 110009418670
  • Peer Reviewed
• [Journal Article] Analysis of methane production by microorganisms indigenous to a depleted oil reservoir for application in Micobial Enchance Oil Recoverly (2012)
  • Author(s): 小林肇、川口秀夫、遠藤啓太、眞弓大介、坂田将、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 113 Pages: 84-87
  • Peer Reviewed
• [Presentation] 地下油田内微生物によるメタン生産 (2010)
  • Author(s): 小林肇、川口秀夫、佐藤光三、眞弓大介、坂田将、五十嵐雅之、宮川喜洋、前田治男
  • Organizer: 第2回メタンハイドレート総合シンポジウム
  • Place of Presentation: 産業技術総合研究所臨海副都心センター(東京)
  • Year and Date: 2010-12-02
• [Presentation] 地下油田内微生物によるメタン生産 (2010)
  • Author(s): 小林肇、川口秀夫、佐藤光三、眞弓大介、坂田将、五十嵐雅之、宮川喜洋、前田治男
  • Organizer: 第2回メタンバイトレート総合シンポジウム
  • Place of Presentation: 産業技術総合研究所臨海副都心センター、東京
  • Year and Date: 2010-12-02
• [Presentation] Study to enhance methanogenesis by subsurface microorganisms within petroleum reservoirs (2010)
  • Author(s): 小林肇、遠藤啓太、坂田将、眞弓大介、川口秀夫、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Organizer: 日本微生物生態学会第26回大会
  • Place of Presentation: 筑波大学、茨城
  • Year and Date: 2010-12-02
• [Presentation] Study to enhance methanogenesis by subsurface microorganisms within petroleum reservoirs (2010)
  • Author(s): 小林肇、遠藤啓太、坂田将、眞弓大介、川口秀夫、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Organizer: 日本微生物生態学会第26回大会
  • Place of Presentation: 筑波大学(茨城)
  • Year and Date: 2010-11-25
• [Presentation] 枯渇油田における微生物を利用した原油分解メタン生成に関する研究 (2010)
  • Author(s): 小林肇、遠藤啓太、川口秀夫、佐藤光三、前田治男、坂田将
  • Organizer: 石油技術協会春季講演会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2010-06-09
• [Presentation] 枯渇油田における微生物を利用した原油分解メタン生成に関する研究 (2010)
  • Author(s): 小林肇、遠藤啓太、川口秀夫、佐藤光三、前田治男、坂田将
  • Organizer: 石油技術協会春季講演会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2010-06-09
• [Presentation] 地下微生物圏の二酸化炭素への応答 (2009)
  • Author(s): 小林肇、遠藤啓太、川口秀夫、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Organizer: 日本微生物生態学会第25回大会
  • Place of Presentation: 広島大学(広島)
  • Year and Date: 2009-11-22
• [Presentation] 地下微生物圏の二酸化炭素への応答 (2009)
  • Author(s): 小林肇
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2009-11-22
• [Presentation] 天然ガスde novo生産へ向けた地下微生物群の研究 (2009)
  • Author(s): 遠藤啓太、小林肇、川口秀夫、五十嵐雅之、宮川喜洋、前田治男、佐藤光三
  • Organizer: 日本微生物生態学会第25回大会
  • Place of Presentation: 広島大学(広島)
  • Year and Date: 2009-11-21
• [Presentation] 天然ガスde novo生産へ向けた地下微生物群の研究 (2009)
  • Author(s): 遠藤啓太
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2009-11-21
• [Remarks]
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• [Remarks]
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• [Remarks]
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