| Project/Area Number |
21880023
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ANDO Akinori Kyoto University, 微生物科学寄附研究部門, 助教 (10537765)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
|
|---|
| Keywords | 脂質漏出性糸状菌 / Mortzerella alpina / 高度不飽和脂肪酸 / 相同組換え / Mortierella alpina / 相同組み換え / Agrobacterium tumefaciens |
|---|
| Research Abstract |
We have isolated the oleaginous filamentous fungus Mortierella alpina 1S-4 from soil and studied the biosynthetic pathway of polyunsaturated fatty acid. We created an oil-excreting fungus V6 through the chemical mutation of M. alpina 1S-4. The polar lipids consisting the membrane of lipid particle excreted by V6 were analyzed. The presences of three kinds of glycolipids (sample 1, sample 2 and sample 3) were found in the extracellular lipids. Based on their mobility on TLC, sample 2 and sample 3 were assumed to be cerebrosides and sample 1 was assumed to be steryl glycoside. Sample 1 was identified to be a mixture of campesteryl glucoside, stigmasteryl glucoside and sitosteryl glucoside. Sample 2 and sample 3 were identified to be 1-O-β-D-glucopyranosyl-2-N-(2'-hydorxyalkanoyl)-9-methyl-trans-4, trans-8-sphingadienines and 1-O-β-D-glucopyranosyl -2-N-(2'-hydorxyalkanoyl)-8-hydroxy-9-methyl-4,8-sphingadienines, respectively.
Furthermore, we found the vigorous growth and weakness of cell wall of M. alpina lead to excrete lipids. On the other hand, we constructed ku80-disrupted strain by single-crossover homologous recombination to improve the efficiency of gene targeting and multi-auxotroph mutants to investigate multiple gene function at a time.
|
