Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability

Research Project

Project/Area Number	21880029
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	General fisheries
Research Institution	Hiroshima University
Principal Investigator	HIRAYAMA Makoto Hiroshima University, 大学院・生物圏科学研究科, 助教 (40535465)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000) Fiscal Year 2010: ¥1,222,000 (Direct Cost: ¥940,000、Indirect Cost: ¥282,000) Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
Keywords	遺伝子改変技術 / 海藻 / 細胞再構築現象 / ハネモ / 遺伝子改変
Research Abstract	Reporter mRNA encoding Renilla luciferase was introduced into the sub-protoplasts, which were regenerated by squeezing out from the coenocytic green alga Bryopsis plumosa, using conventional transgenic methods with polyethyleneglycol and cationic lipid or a new method of which the reporter mRNA was added into the squeezing medium. The luciferase activities were detected in those sub-protoplasts introduced by the new method as well as by the conventional methods. These results indicated that a new easy transgenic technique in coenocytic green algae could be established.

Report