Exploratory and functional analysis of new regulatory factor on energy metabolism

• Project/Area Number: 21880037
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: University of Miyazaki
• Principal Investigator: AKIEDA Sayaka University of Miyazaki, フロンティア科学実験総合センター, 助教 (20549076)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: 脂肪組織 / エネルギー代謝調節 / 肥満 / 生理活性物質 / メタボリック症候群 / 高脂肪食耐性動物 / 脂肪細胞 / マクロファージ

Research Abstract

High fat diet (HFD) is well known to be one of the contributing factors for the development of obesity. When rats were bred with 60% HFD, most of them show a state of increased body weight with high adiposity. However, we found that 10% rats bred with HFD demonstrate obesity-resistant [diet resistant (DR)] that their weighs are less than those of either diet-induced obesity (DIO) or control rats. We found that the mesenteric fat mass of DR rats was significantly less than that of DIO rats and similar to that of control rats, even though there were no differences in spontaneous activity, calorie intake, respiratory quotient and body temperature between DIO and DR rats. To examine the gene expressions specific for the mesenteric adipose tissues of DR rats, we performed DNA microarray analysis. A new bioactive peptide (gene X) and its receptor (gene Y) were found as specific expressed genes in three out of seven DR rats, and these genes were expressed in the macrophage of adipose tissue. We will examine the effects of co-culturing of rat primary mesenteric adipocytes with macrophages expressing gene X and gene Y on adipogenesis. Moreover, we are preparing the analysis in double (gene X and Y) knock-in Tg rats.

Research Products

• [Journal Article] Sex differences in energy metabolism in pre-pubescent, early pubescent and adult rats. (2011)
  • Author(s): Mutoh E, Senba K, Akieda-Asai S, Miyashita A, Poleni PE., Date Y
  • Journal Title: Obesity Research & Clinical 5
  • Peer Reviewed
• [Journal Article] Sex differences in energy metabolism in pre-pubescent, early pubescent and adult rats (2011)
  • Author(s): Mutoh E, Senba K, Akieda-Asai S, Miyashita A, Poleni PE, Date Y
  • Journal Title: Obesity Research & Clinical Practice Volume: 5
  • Peer Reviewed
• [Journal Article] SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals. (2010)
  • Author(s): Akieda-Asai S, Kahyo T, Zaima N, Yao I, Hatanaka T, Ikegami K, Iemura S, Sugiyama S, Yokozeki T, Eishi Y, Koike M, Ikeda K, Chiba T, Yamaza H, Shimokawa I, Song SY, Matsuno A, Mizutani A, Sawabe M, Chao MV, Tanaka M, Kanaho Y, Natsume T, Date Y, McBurney MW, Guarente L, Setou M
  • Journal Title: PLoS One 5(7)
  • Peer Reviewed
• [Journal Article] SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals. (2010)
  • Author(s): Akieda-Asai S, Kahyo T, Zaima N, Yao I, Hatanaka T, Ikegami K, Iemura S, Sugiyama S, Yokozeki T, Eishi Y, Koike M, Ikeda K, Chiba T, Yamaza H, Shimokawa I, Song SY, Matsuno A, Mizutani A, Sawabe M, Chao MV, Tanaka M, Kanaho Y, Natsume T, Date Y, McBurney MW, Guarente L, Setou M
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry. (2009)
  • Author(s): Ageta H, Akieda-Asai S, Sugiura Y, Goto-Inoue N, Zaima N, Setou M
  • Journal Title: Medical Molecular Morphology 42(1) Pages: 16-23
  • Peer Reviewed
• [Journal Article] Effects of transmaternal exposure to genistein in Hatano high- and low-avoidance rats. (2009)
  • Author(s): Ohta R, Shirota M, Kanazawa Y, Shindo T, Furuya M, Seki T. Ono H, Kojima K, Akieda-Asai S, Watanabe G, Taya K
  • Journal Title: Experimental Animals 58(5) Pages: 471-479
  • Peer Reviewed
• [Journal Article] 食行動を制御する臓器間クロストーク (2009)
  • Author(s): 秋枝さやか, 伊達紫
  • Journal Title: 化学と生物 47(11) Pages: 750-755
  • NAID: 10026436816
• [Journal Article] 食行動を制御する臓器間クロストーク (2009)
  • Author(s): 秋枝さやか, 伊達紫
  • Journal Title: 化学と生物 Vol.47 Pages: 750-755
  • NAID: 10026436816
• [Journal Article] Effects of transmaternal exposure to genistein in Hatano high- and low -avoidance rats (2009)
  • Author(s): Ohta R, Shirota M, Kanazawa Y, Shindo T, Furuya M, Seki T, Ono H, Kojima K, Asai-Akieda S, Watanabe G, Taya K
  • Journal Title: Experimental Animals Vol.58 Pages: 471-479
  • Peer Reviewed
• [Presentation] Sex difference in pre-puberty, early puberty and adult rats on energy metabolism (2010)
  • Author(s): Date Y, Mutoh E, Akieda-Asai S, Senba K
  • Organizer: 第14回国際内分泌学会
  • Place of Presentation: 京都
  • Year and Date: 2010-03-30
• [Presentation] Sex difference in pre-puberty, early puberty and adult rats on energy metabolism (2010)
  • Author(s): Date Y, Mutoh E, Asai-Akieda S, Senba K
  • Organizer: 第14回国際内分泌学会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2010-03-30
• [Presentation] Endocrine mechanisms responsible for different ovarian follicular development during estrous cycle between high- and low-avoidance rats (2010)
  • Author(s): Horii Y, Jaroenporn S, Akieda-Asai S, Wang K, Ohta R, Shirota M, Watanabe G, Taya K
  • Organizer: 第14回国際内分泌学会
  • Place of Presentation: 京都
  • Year and Date: 2010-03-27
• [Presentation] Endocrine mechanisms responsible for different ovarian follicular development during estrous cycle between high- and low-avoidance rats (2010)
  • Author(s): Horii Y, Jaroenporn S, Asai-Akieda S, Wang K, Ohta R, Shirota M, Watanabe G, Taya K
  • Organizer: 第14回 国際内分泌学会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2010-03-27
• [Presentation] 長寿遺伝子SIRT1によるホルモン分泌制御機構 (2010)
  • Author(s): 秋枝さやか
  • Organizer: 第3回FANTASY
  • Place of Presentation: 東京
  • Year and Date: 2010-02-07
• [Presentation] 長寿遺伝子SIRT1によるホルモン分泌制御機構 (2010)
  • Author(s): 秋枝さやか
  • Organizer: 第3回FANTASY
  • Place of Presentation: リーガロイヤルホテル(東京)
  • Year and Date: 2010-02-07
• [Presentation] 過剰エネルギー摂取における性差 (2010)
  • Author(s): 秋枝さやか, 武藤絵里, 仙波和代, Paul-Emile Poleni, 伊達紫
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広
• [Presentation] SIRT1 deacetylates PIP5K-gamma to enhance exocytosis of thyroid-stimulating hormone (2009)
  • Author(s): Setou M, Akieda-Asai S, Kahyo T, Zaima N, Yao I, Date Y, McBurney MW, Guarente L
  • Organizer: Exciting Biologies : Biology in Balance
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2009-10-09
• [Presentation] SIRT1 deacetylates PIP5K-gamma to enhance exocytosis of thyroid-stimulating hormone (2009)
  • Author(s): Setou M, Asai-Akieda S, Kahyo T, Zaima N, Yao I, Date Y, McBurney M W, Guarente L
  • Organizer: Exciting Biologies : Biology in Balance
  • Place of Presentation: Sofitel Hotel(アルゼンチン・ブエノスアイレス)
  • Year and Date: 2009-10-09
• [Presentation] 狂犬病ウイルス感染における骨髄系樹状細胞の機能解析 (2009)
  • Author(s): 仙波和代, 武藤絵里, 秋枝さやか, 野口賀津子, 伊波英克, 伊達紫, 西園晃
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] Sex difference in pre-puberty, early puberty and adult rats on energy metabolism (2009)
  • Author(s): 武藤絵里, 秋枝さやか, 仙波和代, 伊達紫
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] SIRT1 regulates thyroid-stimulating hormone secretion through phosphatidylinositol 4-phosphate 5-kinase gamma (2009)
  • Author(s): Akieda-Asai S, Kahyo T, Yao I, Zaima N, Semba K, Mutoh E, Date Y, Setou M
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] SIRT1 regulates thyroid-stimulating hormone secretion through phosphatidylinositol 4-phosphate 5-kinase gamma (2009)
  • Author(s): Asai-Akieda S, Kahyo T, Yao I, Zaima N, Semba K, Mutoh E, Date Y, Setou M
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: シーガイア ワールドコンベンションセンター(宮崎)
  • Year and Date: 2009-05-11
• [Presentation] Sex difference in pre-puberty, early puberty and adult rats on energy metabolism (2009)
  • Author(s): 武藤絵里, 秋枝さやか, 仙波和代, 伊達紫
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: シーガイア ワールドコンベンションセンター(宮崎)
  • Year and Date: 2009-05-11
• [Presentation] 狂犬病ウイルス感染における骨髄系樹状細胞の機能解析 (2009)
  • Author(s): 仙波和代, 武藤絵里, 秋枝さやか, 野口賀津子, 伊波英克, 伊達紫, 西園晃
  • Organizer: 分子生物学会第9回春季シンポジウム
  • Place of Presentation: シーガイア ワールドコンベンションセンター(宮崎)
  • Year and Date: 2009-05-11
• [Remarks] ホームページ等
  • URL: http://www.med.miyazaki-u.ac.jp/peptides/date/jp/
• [Remarks]
  • URL: http://www.med.miyazaki-u.ac.jp/peptides/date/jp/