| Project/Area Number |
21880048
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
|
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| Research Institution | Kobe Pharmaceutical University |
|---|
Principal Investigator |
SHITAN Nobukazu Kobe Pharmaceutical University, 薬学部, 助教 (20547880)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,808,000 (Direct Cost: ¥2,160,000、Indirect Cost: ¥648,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,495,000 (Direct Cost: ¥1,150,000、Indirect Cost: ¥345,000)
|
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| Keywords | 植物 / アルカロイド / トランスポーター / ニコチン / 転流 / MATE / プリンパーミエース |
|---|
| Research Abstract |
Alkaloids play a key role in the plants defense mechanism. The major alkaloid of the Nicotiana species, nicotine, is translocated from the root to the vacuoles of leaves. In this study, two transporters, C215 and T408, were analyzed. C215 is expressed in the tonoplast of leaves, and transported nicotine. Overexpression of C215 induced the nicotine production. T408 is localized to plastid and play an important role in nicotine synthesis. These knowledge might enhance the production of valuable secondary metabolites and the molecular breeding of plant which is more resistant to insects.
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