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Development of functional oligosaccharides using glycoside hydrolases from basidiomycetes

Research Project

Project/Area Number 21880050
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

KONNO Naotake  (財)岩手生物工学研究センター, 生物資源研究部, 研究員 (60549880)

Co-Investigator(Renkei-kenkyūsha) SAKAMOTO Yuichi  財団法人岩手生物工学研究センター, 生物資源研究部, 主任研究員 (80390889)
YAMADA Hidetoshi  財団法人岩手生物工学研究センター, 生物資源研究部, 研究員 (70511955)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywordsバイオマス / 糖質分解酵素 / 担子菌 / オリゴ糖 / 機能性食品 / シイタケ
Research Abstract

The cell walls of fungi are constructed mainly from chitin and β-1,3/1,6-glucan, and fungi are known to produce enzymes associated with cell-wall polysaccharides. In this study, we purified β-1,3- and β-1,6-glucanase, GLU1 and LePUS30A, from the fruiting body of Lentinula edodes (Shiitake mushroom). GLU1 specifically degraded β-1,3-glucans with an endo-type manner. LePUS30A catalyzed depolymerization of β-1,6-glucan endolytically, and the enzymatic products induced TNF α production.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Anendo-β-1, 6-glucanase involved in Lentinula edodes fruiting body autolysis.

    • Author(s)
      Naotake Konno, Yuichi Sakamoto
    • Journal Title

      Applied Microbiology and Biotechnology (in press)

    • Related Report
      2010 Final Research Report
  • [Journal Article] An endo-β-1,6-glucanase involved in Lentinula edodes fruiting body autolysis

    • Author(s)
      Naotake Konno, Yuichi Sakamoto
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 担子菌シイタケ由来β-1,6グルカナーゼの単離と機能解析2010

    • Author(s)
      金野尚武, 坂本裕一
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      静岡県、グランシップ
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Glucanase involved in Lentinula edodes fruiting body senescence2010

    • Author(s)
      Naotake Konno, Yuichi Sakamoto
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      千葉県、幕張メッセ
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] シイタケ由来グルカナーゼ群の特性解析2010

    • Author(s)
      金野尚武
    • Organizer
      農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス(東京都)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 担子菌シイタケ由来β-1,6グルカナーゼの単離と機能解析2010

    • Author(s)
      金野尚武,坂本裕一
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] Glucanase involved in Lentinula edodes fruiting body senescence2010

    • Author(s)
      Naotake Konno, Yuichi Sakamoto
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      千葉
    • Related Report
      2010 Final Research Report
  • [Presentation] シイタケ由来グルカナーゼ群の特性解析2010

    • Author(s)
      金野尚武, 坂本裕一
    • Organizer
      農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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