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Enhancement of gene targeting via cell cycle regulation in higher plants

Research Project

Project/Area Number 21880054
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

ENDO Masaki  National Institute of Agrobiological Sciences, 遺伝子組換え技術研究ユニット, 任期付研究員 (40546371)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,574,000 (Direct Cost: ¥1,980,000、Indirect Cost: ¥594,000)
Fiscal Year 2010: ¥1,222,000 (Direct Cost: ¥940,000、Indirect Cost: ¥282,000)
Fiscal Year 2009: ¥1,352,000 (Direct Cost: ¥1,040,000、Indirect Cost: ¥312,000)
Keywords植物 / ジーンターゲッティング / 相同組換え
Research Abstract

Gene targeting is achieved through the homologous recombination between endogenous genome and foreign DNA, which has homology to endogenous genome. Homologous recombination is one of the DNA double strand break repair mechanism. In contrast to prokaryotes and lower eukaryotes, gene targeting is infrequent in higher plants and foreign DNA integrates into plant genome mainly via non-homologous end joining because of low homologous recombination frequency. Depend on the cell cycle phase, the choice of one of the DNA repair pathway over the other will secure genome stability maintenance and homologous recombination is an efficient way to maintain genome stability at a post-replication stage. In this study, we analyzed the Arabidopsis mutants of cell cycle regulation factors and revealed that the mutant, which lacks positive regulator of G1/S transition showed low homologous recombination frequency.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2010 2009 Other

All Journal Article (2 results) Presentation (12 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] 遺伝子ターゲッティング技術を利用したBS耐性イネの分子育種2010

    • Author(s)
      雑賀啓明,遠藤真咲,土岐精一
    • Journal Title

      日本農薬学会誌 35(2)

      Pages: 172-175

    • NAID

      110007651173

    • Related Report
      2010 Final Research Report
  • [Journal Article] 遺伝子ターゲッティング技術を利用したBS耐性イネの分子育種2010

    • Author(s)
      雑賀啓明
    • Journal Title

      日本農薬学会誌

      Volume: 35 Pages: 172-175

    • Related Report
      2010 Annual Research Report
  • [Presentation] Delivery of T-DNA to DNA double stranded breaks ; Mechanism and application.2010

    • Author(s)
      遠藤真咲
    • Organizer
      31th International Crown Gall Conference.
    • Place of Presentation
      UC Berkeley, CA
    • Year and Date
      2010-11-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Delivery of T-DNA to DNA double stranded breaks ; Mechanism and application2010

    • Author(s)
      Endo, M
    • Organizer
      31th International Crown Gall Conference
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物のDNA2重鎖切断修復の細胞周期による制御2010

    • Author(s)
      遠藤真咲
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      日中部大学
    • Year and Date
      2010-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物のDNA2重鎖切断修復の細胞周期による制御2010

    • Author(s)
      土岐精一
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of CAF-1 mutants in rice2010

    • Author(s)
      Abe K
    • Organizer
      2nd International Meeting on Plant DNA Repair and Recombination
    • Place of Presentation
      アシロマ,カリフォルニア
    • Year and Date
      2010-03-02
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナにおける細胞周期制御因子の改変と遺伝子相同組換えへの影響2009

    • Author(s)
      遠藤真咲
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Application possibility of CAF-1 mutant for gene targeting in rice2009

    • Author(s)
      Abe K
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナにおける細胞周期制御因子の改変と遺伝子相同組換えへの影響2009

    • Author(s)
      遠藤真咲、土岐精一
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Homologous recombination and gene targeting in rice.2009

    • Author(s)
      Toki S
    • Organizer
      9^<th> International Plant Molecular Biology (IPMB) Congress.
    • Place of Presentation
      セントルイス
    • Year and Date
      2009-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Homologous recombination and gene targeting in rice.2009

    • Author(s)
      Toki S, Endo M, Saika H, Abe K, Osakabe K.
    • Organizer
      9^<th> International Plant Molecular Biology Congress.
    • Place of Presentation
      セントルイス(アメリカ)
    • Year and Date
      2009-10-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] OsCDKB2 knockdown induces endomitosis in rice.2009

    • Author(s)
      遠藤真咲
    • Organizer
      9^<th> International Plant Molecular Biology (IPMB) Congress.
    • Place of Presentation
      セントルイス
    • Year and Date
      2009-10-27
    • Related Report
      2010 Final Research Report
  • [Presentation] OsCDKB2 knockdown induce endomitosis in rice.2009

    • Author(s)
      Endo M, Nakayama S, Toki S.
    • Organizer
      9^<th> International Plant Molecular Biology Congress.
    • Place of Presentation
      セントルイス(アメリカ)
    • Year and Date
      2009-10-27
    • Related Report
      2009 Annual Research Report
  • [Book] Regulatory mechanisms of homologous recombination in higher plants-Plant Developmental Biology-Biotechnological Perspectives Volume 2(4(19))2010

    • Author(s)
      Osakabe K., Abe K., Endo M., Toki S
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Plant Developmental Biology-Biotechnological Perspective Vol.2 ?Regulation of Mechanisms of Homologous Recombination in Higher Plants.2010

    • Author(s)
      Osakabe K., Abe K., Endo M., Toki S
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Biotechnological Perspectives Volume22010

    • Author(s)
      Osakabe K.
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Book] Plant Developmental Biology-Biotechnological Perspective Vol.22010

    • Author(s)
      Osakabe K, Abe K, Endo M, Toki S.
    • Total Pages
      423
    • Publisher
      Springer
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] イネ科植物の核内の染色体数を倍加させる薬剤及びその利用2009

    • Inventor(s)
      遠藤真咲、雑賀啓明、中山繁樹、梅田正明、土岐精一
    • Industrial Property Rights Holder
      遠藤真咲、雑賀啓明、中山繁樹、梅田正明、土岐精一
    • Filing Date
      2009-12-25
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] イネ科植物の核内の染色体数を倍加させる薬剤及びその利用2009

    • Inventor(s)
      遠藤真咲、雑賀啓明、中山繁樹、梅田正明、土岐精一
    • Industrial Property Rights Holder
      独立行政法人農業生物資源研究所、国立大学法人奈良先端科学技術大学院大学
    • Filing Date
      2009-12-25
    • Related Report
      2009 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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