Hormonal regulation and molecular mechanisms of insect host-symbiont crosstalk

• Project/Area Number: 21880056
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: FUTAHASHI Ryo National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究員 (50549889)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: 共生細菌 / 絶食 / 昆虫ホルモン / EST / 体色 / 脱皮ホルモン / 体色変化

Research Abstract

I found that starvation induced by molting hormone is the main cause of reduction of the amount of symbiotic bacteria in hemipteran insects. Also I succeeded in obtaining many novel genes involved in insect symbiosis by the construction of EST database and quantitative expression analysis. Moreover, I established RNAi method for stinkbug insects. Identification of novel symbiosis-associated genes and establishment of functional analysis will allow significant advance in analysis of the molecular mechanisms of host-symbiont crosstalk.

Research Products

• [Journal Article] Laccase2 is required for cuticular pigmentation in stinkbugs. (2011)
  • Author(s): Futahashi R., Tanaka K., Matsuura Y., Tanahashi M., Kikuchi Y., Fukatsu T.
  • Journal Title: Insect Biochemistry and Molecular Biology. 41巻 Pages: 191-196
  • Peer Reviewed
• [Journal Article] RNA interference in Lepidoptera : an overview of successful and unsuccessful studies and implications for experimental design. (2011)
  • Author(s): Terenius O., ほか(23番目の著者 Futahashi R.).
  • Journal Title: Journal of Insect Physiology 57巻 Pages: 231-245
  • Peer Reviewed
• [Journal Article] Laccase2 is required for cuticular pigmentation in stinkbugs. (2011)
  • Author(s): Futahashi R., Tanaka K., Matsuura Y., Tanahashi M., Kikuchi Y., Fukatsu T.
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 41 Pages: 191-196
  • Peer Reviewed
• [Journal Article] RNA interference in Lepidoptera : an overview of successful and unsuccessful studies and implications for experimental design. (2011)
  • Author(s): Terenius O., ほか(including Futahashi R.)
  • Journal Title: Journal of Insect Physiology Volume: 57 Pages: 231-245
  • Peer Reviewed
• [Journal Article] Repression of tyrosine hydroxylase is responsible for the sex-linked chocolate mutation of the silkworm., Bombyx mori. (2010)
  • Author(s): Liu C., Yamamoto K., Cheng T.C., Kadono-Okuda K., Narukawa J., Liu S.P., Han Y., Futahashi R., Kidokoro K., Noda H.,ほか.
  • Journal Title: Proc Natl Acad Sci USA 107巻 Pages: 12980-12985
  • Peer Reviewed
• [Journal Article] Species-specific coordinated gene expression and trans-regulation of larval color pattern in three swallowtail butterflies. (2010)
  • Author(s): Shirataki H., Futahashi R., Fujiwara H.
  • Journal Title: Evolution and Development 12巻 Pages: 305-314
  • Peer Reviewed
• [Journal Article] Caterpillar color patterns are determined by a two-phase melanin gene pre-patterning process : new evidence from tan and laccase2. (2010)
  • Author(s): Futahashi R., Banno Y., Fujiwara, H.
  • Journal Title: Evolution and Development 12巻 Pages: 157-167
  • Peer Reviewed
• [Journal Article] Repression of tyrosine hydroxylase is responsible for the sex-linked chocolate mutation of the silkworm, Bombyx mori. (2010)
  • Author(s): Liu C., Yamamoto K., Cheng T.C., Kadono-Okuda K., Narukawa J., Liu S.P., Han Y., Futahashi R., Kidokoro K., Noda H., ほか
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 12980-12985
  • Peer Reviewed
• [Journal Article] Species-specific coordinated gene expression and trans-regulation of larval color pattern in three swallowtail butterflies. (2010)
  • Author(s): Shirataki H., Futahashi R., Fujiwara H.
  • Journal Title: Evolution and Development Volume: 12 Pages: 305-314
  • Peer Reviewed
• [Journal Article] Caterpillar color patterns are determined by a two-phase melanin genepre-patterning process : new evidence from tan and laccase2 (2010)
  • Author(s): Futahashi R., Banno Y., Fujiwara, H.
  • Journal Title: EVOLUTION & DEVELOPMENT 12 Pages: 157-167
  • Peer Reviewed
• [Presentation] Development and evolution of color pattern diversity in dragonflies. (2011)
  • Author(s): Futahashi R., Fukatsu T.
  • Organizer: 第20回理研CDB会議
  • Place of Presentation: 神戸
  • Year and Date: 2011-02-23
• [Presentation] EST-based screening for genes involved in host-symbiont interactions in the bean bug Riptortus pedestris. (2010)
  • Author(s): Futahashi R., Tanaka K., Tanahashi. M., Kikuchi Y., Fukatsu T.
  • Organizer: 国際生物学賞記念シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2010-12-07
• [Presentation] トンボにおけるDNA解析の現状と最新の知見について (2010)
  • Author(s): 二橋亮
  • Organizer: 2008年度日本蜻蛉学会大会
  • Place of Presentation: 小田原(招待講演)
  • Year and Date: 2010-11-14
• [Presentation] Color-pattern evolution and speciation in dragonflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: カイコゲノム関連研究集会
  • Place of Presentation: つくば
  • Year and Date: 2010-11-10
• [Presentation] Color-pattern evolution and speciation in dragonflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: カイコゲノム関連研究集会
  • Place of Presentation: つくば(招待講演)
  • Year and Date: 2010-11-10
• [Presentation] トンボにおける色彩多型の解明に向けて (2010)
  • Author(s): 二橋亮・深津武馬
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] トンボにおける体色・翅色多型の進化機構 (2010)
  • Author(s): 二橋亮・深津武馬
  • Organizer: 新学術領域公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-09-17
• [Presentation] Comparison of color pattern evolution between dragonflies & butterflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: 第6回国際蝶類学会
  • Place of Presentation: エドモントン(カナダ)
  • Year and Date: 2010-06-30
• [Presentation] Comparison of color pattern evolution between dragonflies & butterflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: 第6回国際蝶類学会
  • Place of Presentation: エドモントン(カナダ)(招待講演)
  • Year and Date: 2010-06-30
• [Presentation] チョウとトンボに見られる色彩多様性の進化機構 (2010)
  • Author(s): 二橋亮
  • Organizer: 植物色素談話会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-06-19
• [Presentation] 国内のギンヤンマ属に見られる種間浸透の実体 (2010)
  • Author(s): 二橋亮
  • Organizer: 昆虫DNA研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-05-22
• [Presentation] トンボの種分化と色彩多様性 (2009)
  • Author(s): 二橋亮
  • Organizer: 昆虫ワークショップ2009
  • Place of Presentation: 博多
  • Year and Date: 2009-10-29
• [Presentation] チョウ目昆虫の幼虫紋様形成機構:アゲハとカイコの比較解析研究 (2009)
  • Author(s): 二橋亮・藤原晴彦
  • Organizer: 日本遺伝学会第81回大会(招待講演)
  • Place of Presentation: 松本
  • Year and Date: 2009-09-18
• [Presentation] カイコの体色変異体(ch,so,ro)の原因遺伝子はメラニン合成遺伝子である (2009)
  • Author(s): 二橋亮・藤原晴彦
  • Organizer: 昆虫ポストゲノム研究会(招待講演)
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-09
• [Presentation] トンボの翅色・体色の色彩多型メカニズム (2009)
  • Author(s): 二橋亮
  • Organizer: 第11回日本進化学会札幌大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-03
• [Presentation] Microarray-based gene expression profiles in stage-specific larval body markings of the swallowtail butterfly, Papilio xuthus (2009)
  • Author(s): Futahashi R., Fujiwara H.
  • Organizer: 第8回国際鱗翅目分子生物学会
  • Place of Presentation: ハニア(ギリシア)
  • Year and Date: 2009-08-24
• [Book] 生物学辞典 (2010)
  • Author(s): 石川統編(二橋亮, 分担執筆)
  • Total Pages: 1616
  • Publisher: 東京化学同人
• [Book] 分子昆虫学 (2009)
  • Author(s): 二橋亮(分担執筆)
  • Total Pages: 448
  • Publisher: 共立出版
• [Remarks] ホームページ等
  • URL: http://staff.aist.go.jp/t-fukatsu/Futahashi_right.html