Network Calcium Imaging in Suprachiasmatic Nucleus
| Project/Area Number |
21890003
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
ENOKI Ryosuke Hokkaido University, 大学院・医学研究科, 助教 (00528341)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000)
Fiscal Year 2010: ¥1,248,000 (Direct Cost: ¥960,000、Indirect Cost: ¥288,000)
Fiscal Year 2009: ¥1,378,000 (Direct Cost: ¥1,060,000、Indirect Cost: ¥318,000)
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| Keywords | 視交叉上核 / 概日リズム / カルシウムイメージング / 共焦点顕微鏡 / 神経回路網 / 光イメージング / カルシウム / 共焦点レーザー顕微鏡 / 多光子レーザー顕微鏡 / シナプス / 2光子励起顕微鏡 / 時計遺伝子 |
|---|
| Research Abstract |
The present study was carried out to visualize the spatiotemporal patterns of neuronal network activities in suprachiasmatic nucleus, the center for mammalian circadian clock. To this goal, I have developed fast scanning single or multi-photon confocal microscopy and methods to load calcium sensitive dye in large neuronal population in suprachiasmatic nucleus. I successfully recorded synaptically-induced or spontaneous calcium concentration changes from hundreds of neurons in deep brain tissue at high spatial (single cell level) and temporal resolutions (millisecond scale).
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Report
(3 results)
Research Products
(11 results)
