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Network Calcium Imaging in Suprachiasmatic Nucleus

Research Project

Project/Area Number 21890003
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionHokkaido University

Principal Investigator

ENOKI Ryosuke  Hokkaido University, 大学院・医学研究科, 助教 (00528341)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000)
Fiscal Year 2010: ¥1,248,000 (Direct Cost: ¥960,000、Indirect Cost: ¥288,000)
Fiscal Year 2009: ¥1,378,000 (Direct Cost: ¥1,060,000、Indirect Cost: ¥318,000)
Keywords視交叉上核 / 概日リズム / カルシウムイメージング / 共焦点顕微鏡 / 神経回路網 / 光イメージング / カルシウム / 共焦点レーザー顕微鏡 / 多光子レーザー顕微鏡 / シナプス / 2光子励起顕微鏡 / 時計遺伝子
Research Abstract

The present study was carried out to visualize the spatiotemporal patterns of neuronal network activities in suprachiasmatic nucleus, the center for mammalian circadian clock. To this goal, I have developed fast scanning single or multi-photon confocal microscopy and methods to load calcium sensitive dye in large neuronal population in suprachiasmatic nucleus. I successfully recorded synaptically-induced or spontaneous calcium concentration changes from hundreds of neurons in deep brain tissue at high spatial (single cell level) and temporal resolutions (millisecond scale).

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (9 results)

  • [Journal Article] Expression of long-term plasticity at indivisual synapses in hippcampus is graded, bidirectional and mainly presynaptic. Optical quantal analysis.2009

    • Author(s)
      R.Enoki, Y.Hu, D.Hamilton, A.Fine
    • Journal Title

      NEURON 62(2)

      Pages: 242-253

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of long-term plasticity at indivisual synapses in hippcampus is graded, bidirectional and mainly presynaptic. Optical quantal analysis2009

    • Author(s)
      R.Enoki, Y.Hu, D.Hamilton, A.Fine
    • Journal Title

      NEURON 62(2)

      Pages: 242-253

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Multi-photon imaging ofsynaptic transmission and plasticity in the brain2011

    • Author(s)
      榎木亮介
    • Organizer
      2nd International Symposium on Photonic Bioimaging
    • Place of Presentation
      ニセコヒルトンビレッジ(北海道)
    • Year and Date
      2011-02-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Multi-photon imaging of synaptic transmission and plasticity in the brain2011

    • Author(s)
      榎木亮介
    • Organizer
      2^<nd> International Symposium on Photonic Bioimaging
    • Place of Presentation
      ニセコヒルトンビレッジ(北海道)
    • Year and Date
      2011-02-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multi-photon analysis ofsynaptic transmission and plasticity in the brain2010

    • Author(s)
      榎木亮介
    • Organizer
      第八回未来創薬イノベーション国際シンポジウム
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2010-09-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Multi-photon analysis of synaptic transmission and plasticity in the brain2010

    • Author(s)
      榎木亮介
    • Organizer
      第八回未来創薬イノベーション国際シンポジウム
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2010-09-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Circadian rhythm generation in the suprachiasmatic nucleus of Cry1/Cry2 double deficient mice.2010

    • Author(s)
      小野大輔、榎木亮介、本間さと、本間研一
    • Organizer
      2010包括脳ワークショップ
    • Place of Presentation
      さっぽろ芸文館(北海道)
    • Year and Date
      2010-07-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Circadian rhythm generation in the suprachiasmatic nucleus of Cry1/Cry2 double deficient mice2010

    • Author(s)
      小野大輔、榎木亮介、本間さと、本間研一
    • Organizer
      2010包括脳ワークショップ
    • Place of Presentation
      さっぽろ芸文館(北海道)
    • Year and Date
      2010-07-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 多光子励起顕微鏡による神経活動の可視化解析2010

    • Author(s)
      榎木亮介
    • Organizer
      北海道大学連携研究センターシンポジウム
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2010-03-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 2光子励起顕微鏡よるシナプス可塑性の可視化解析2010

    • Author(s)
      榎木亮介
    • Organizer
      北海道大学第5回未来創薬医療イノベーションセミナー
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2010-01-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Illuminating presynaptic plasticity by two-photon optical quantal analysis.2009

    • Author(s)
      榎木亮介
    • Organizer
      International Symposium on Biological Rhythm.Sapporo.
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2009-08-01
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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