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Development of New Antiviral Therapy Targeting Viroporins

Research Project

Project/Area Number 21890004
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

SUZUKI Tadaki  National Institute of Infectious Diseases, 感染病理部, 任期付研究員 (30527180)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
KeywordsJCウイルス / 進行性多巣性白質脳症 / Viroporin / Agnoprotein / PML
Research Abstract

Viroporins are a group of proteins interact with cellular membranes modifying permeability and which participate in the promotion of release of viral particles from infected cells. The JCV encoded agnoprotein, which is recognized as a viroporin recently, is essential for the viral replication. Here, we demonstrate that JCV viroporin, agnoprotein, interacts with the delta subunit of AP-3. This interaction prevents AP-3-mediated intracellular vesicular trafficking, resulting in plasma membrane permeabilization and facilitating virion release. Furthermore, the disruption of this interaction repressed both the viroporin activity and JC virus replication.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2011 2010 2009

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (27 results)

  • [Journal Article] Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.2011

    • Author(s)
      Sasaki M, Hasebe R, Makino Y, Suzuki T Fukushi H, Okamoto M, Matsuda K, Taniyama H, Sawa H, Kimura T
    • Journal Title

      Genes Cells. 16(4)

      Pages: 343-57

    • NAID

      120005652749

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling.2010

    • Author(s)
      Orba Y, Suzuki T, Makino Y, Kubota K, Tanaka S, Kimura T, Sawa H
    • Journal Title

      J Biol Chem 285(2)

      Pages: 1544-1554

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The human polyoma JC virus agnoprotein acts as a viroporin.2010

    • Author(s)
      Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall WW, Sawa H
    • Journal Title

      PLoS Pathog 6(3)

    • NAID

      120002128723

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low pH-Triggered Model Drug Molecule Release from Virus-Like Particles.2010

    • Author(s)
      Ohtake N, Niikura K, Suzuki T, Nagakawa K, Mikuni S, Matsuo Y, Kinjo M, Sawa H, Ijiro K
    • Journal Title

      Chembiochem. 11(7)

      Pages: 959-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Natalizumab Has No Direct Biological Effect on JC virus Infectivity in Permissive Human Neural Cell Lines.2010

    • Author(s)
      Suzuki T, Yamanouchi S, Sunden Y, Orba Y, Kimura T, Sawa H
    • Journal Title

      J Med Virol 82(7)

      Pages: 1229-35

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein.2010

    • Author(s)
      Hasebe R, Suzuki T, Makino Y, Igarashi M, Yamanouchi S, Maeda A, Horiuchi M, Sawa H, Kimura T
    • Journal Title

      BMC Microbiol. 10

      Pages: 165-165

    • NAID

      120002261736

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of an alpha-helical antimicrobial peptide derived from a novel mouse defensin-like gene.2010

    • Author(s)
      Kawaguchi A, Suzuki T, Kimura T, Sakai N, Ayabe T, Sawa H, Hasegawa H
    • Journal Title

      Biochem Biophys Res Commun. 398(4)

      Pages: 778-84

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Natalizumab has no direct biological effect on JC virus infectivity in permissive human neural cell lines.2010

    • Author(s)
      Suzuki T, Yamanouchi S, Sunden Y, Orba Y, Kimura T, Sawa H
    • Journal Title

      J Med Virol.

      Volume: 82(7) Pages: 1229-1235

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein.2010

    • Author(s)
      Hasebe R, Suzuki T, Makino Y, Igarashi M, Yamanouchi S, Maeda A, Horiuchi M, Sawa H, Kimura T
    • Journal Title

      BMC Microbiol.

      Volume: 10 Pages: 165-165

    • NAID

      120002261736

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of an alpha-helical antimicrobial peptide derived from a novel mouse defensin-like gene.2010

    • Author(s)
      Kawaguchi A, Suzuki T, Kimura T, Sakai N, Ayabe T, Sawa H, Hasegawa H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 398(4) Pages: 778-784

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling2010

    • Author(s)
      Orba Y, Suzuki T, Makino Y, Kubota K, Tanaka S, Kimura T, Sawa H
    • Journal Title

      J Biol Chem 285

      Pages: 1544-1554

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Human Polyoma JC Virus Agnoprotein Acts as a Viroporin2010

    • Author(s)
      Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall WW, Sawa H
    • Journal Title

      PLoS Pathogens 6

    • NAID

      120002128723

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gold Nanoparticle Arrangement on Viral Particles through Carbohydrate Recognition : A Non-Cross-Linking Approach to Optical Virus Detection.2009

    • Author(s)
      Niikura K, Nagakawa K, Ohtake N, Suzuki T, Matsuo Y, Sawa H, Ijiro K
    • Journal Title

      Bioconjug Chem 20(10)

      Pages: 1842-1852

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gold nanoparticle arrangement on viral particles through carbohydrate recognition : A non-crosslinking approach to opticalvirus detection2009

    • Author(s)
      Niikura K, Nagakawa K, Ohtake N, Suzuki T, Matsuo Y, Sawa H, Ijiro K
    • Journal Title

      Bioconjug Chem 20

      Pages: 1848-1852

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ポリオーマウイルス粒子放出の分子機構2010

    • Author(s)
      鈴木忠樹、大場靖子、牧野吉倫、木村享史、佐多徹太郎、長谷川秀樹、澤洋文
    • Organizer
      第9回狂犬病研究会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2010-03-05
    • Related Report
      2010 Final Research Report
  • [Presentation] ポリオーマウイルス粒子放出の分子機構2010

    • Author(s)
      鈴木忠樹、大場靖子、牧野吉倫、木村享史、佐多徹太郎、長谷川秀樹、澤洋文
    • Organizer
      第9回狂犬病研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] ノンエンベロープウイルス粒子放出の分子機構2010

    • Author(s)
      鈴木忠樹
    • Organizer
      (独)産業技術総合研究所ゲノムファクトリー講演会「異分野ネットワークで可能性を切り拓こう-将来の融合研究を見据えて-」
    • Place of Presentation
      札幌(招待講演)
    • Year and Date
      2010-02-19
    • Related Report
      2010 Final Research Report
  • [Presentation] ノンエンベロープウイルス粒子放出の分子機構2010

    • Author(s)
      鈴木忠樹
    • Organizer
      (独)産業技術総合研究所ゲノムファクトリー講演会「異分野ネットワークで可能性を切り拓こう-将来の融合研究を見据えて-」
    • Place of Presentation
      札幌
    • Year and Date
      2010-02-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] チャネル形成ウイルスタンパク質「Viroporin」の機能発現に必要な宿主因子の同定2010

    • Author(s)
      鈴木忠樹、大場靖子、牧野吉倫、木村享史、佐多徹太郎、長谷川秀樹、澤洋文
    • Organizer
      第8回沖縄感染症フォーラム
    • Place of Presentation
      宜野湾市
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Disruption of Intracellular Vesicular Trafficking by Agnoprotein is Essential for Viroporin Activity and JC Virus Replication.2010

    • Author(s)
      Suzuki T, Orba Y, Makino Y, Okada Y, Sunden Y, Kimura T, Hasegawa H, Sata T, Hall WW, Sawa H
    • Organizer
      The 10th International Symposium in NeuroVirology
    • Place of Presentation
      ミラノ、イタリア
    • Related Report
      2010 Final Research Report
  • [Presentation] InvitroにおけるNatalizumabのJC virus感染への影響の検討2010

    • Author(s)
      鈴木忠樹、山内聡子、寸田祐嗣、大場靖子、木村享史、佐多徹太郎、澤洋文
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島市
    • Related Report
      2010 Final Research Report
  • [Presentation] ウイルスタンパク質「Viroporin」の機能制御に関わる宿主因子とウイルス因子の相互作用2010

    • Author(s)
      鈴木忠樹、大場靖子、牧野吉倫、岡田由紀、寸田裕嗣、木村享史、長谷川秀樹、佐多徹太郎、William W.Hall、澤洋文
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Disruption of Intracellular Vesicular Trafficking by Agnoprotein is Essential for Viroporin Activity and JC Virus Replication2010

    • Author(s)
      Suzuki T, Orba Y, Makino Y, Okada Y, Sunden Y, Kimura T, Hasegawa H, Sata T, Hall WW, Sawa H.
    • Organizer
      The 10th International Symposium in Neuro Virology
    • Place of Presentation
      Milan, Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vitroにおけるNatalizumabのJC virus感染への影響の検討2010

    • Author(s)
      鈴木忠樹、山内聡子、寸田祐嗣、大場靖子、木村享史、佐多徹太郎、澤洋立
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島市
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリオーマウイルスの粒子形成機構の解析2010

    • Author(s)
      小林進太郎、鈴木忠樹、大竹範子、永川桂大、新倉謙一、木村享史、澤洋文
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Related Report
      2010 Annual Research Report
  • [Presentation] JCウイルスの粒子形成機構の解析2010

    • Author(s)
      小林進太郎、鈴木忠樹、大竹範子、永川桂大、新倉謙一、木村享史、澤洋文
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Preparation of functionalized virus-like particles enabling pH-mediated release of target molecules2010

    • Author(s)
      Ohtake N, Niikura K, Suzuki T, Mikuni S, Matsuo Y, Nagakawa K, Kinjo M, Sawa H, Ijiro K
    • Organizer
      International Symposium on Joint Research Network for Advanced Material and Devices
    • Place of Presentation
      Tomakomai, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] pHに応答した分子放出のためのウイルスナノカプセルの機能化2010

    • Author(s)
      大竹範子、新倉謙一、鈴木忠樹、三國新太郎、永川桂大、金城政孝、澤洋文、居城邦治
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Viroporin activity of JCV agnoprotein.2009

    • Author(s)
      Suzuki T, Orba Y, Sunden Y, Kimura T, Sawa H
    • Organizer
      The 9th International Symposium in NeuroVirology
    • Place of Presentation
      フロリダ、アメリカ合衆国
    • Related Report
      2010 Final Research Report
  • [Presentation] JCVウイルスAgnoproteinによるウイルス粒子の形態制御2009

    • Author(s)
      鈴木忠樹、仙葉慎吾、大場靖子、寸田祐嗣、木村享史、長嶋和郎、澤洋文
    • Organizer
      第57回日本ウイルス学会総会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] The human Polyoma JC virus acts as a viroporin.2009

    • Author(s)
      鈴木忠樹、大場靖子、岡田由紀、寸田祐嗣、木村享史、田中伸哉、長嶋和郎、澤洋文
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Viroporin activity of JCV agnoprotein2009

    • Author(s)
      Suzuki T, Orba Y, Sunden Y, Kimura T, Sawa H
    • Organizer
      The 9th International Symposium in Neuro Virology
    • Place of Presentation
      Florida, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] Viroporin activity of JCV agnoprotein2009

    • Author(s)
      Suzuki T, Orba Y, Sunden Y, Kimura T, Sawa H
    • Organizer
      The 1st International Young Researcher Seminar in Zoonosis Control 2009
    • Place of Presentation
      Niseko, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] Large T antigen of JC virus promotes viral replication by inducina ATM- and ATR-mediated G2 checkpoint signaling2009

    • Author(s)
      Orba Y, Suzuki T, Kimura T, Sawa H
    • Organizer
      The 1st International Young Researcher Seminar in Zoonosis Control 2009
    • Place of Presentation
      Niseko, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] Gold nanoparticle array based on the surface structure of virus2009

    • Author(s)
      Nagakawa K, Niikura K, Ohtake N, Suzuki T, Matsuo Y, Sawa H, Ijiro K
    • Organizer
      International Symposium on Engineering Neo-Biomimetics
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウイルス微粒子への蛍光タンパク質内包技術の開発2009

    • Author(s)
      大竹範子、新倉謙一、鈴木忠樹、永川桂大、澤洋文、居城邦治
    • Organizer
      第19回バイオ・高分子シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペプチドタグを用いたウイルスナノカプセルへの目的分子内包2009

    • Author(s)
      大竹範子、新倉謙一、鈴木忠樹、永川桂大、澤洋文、居城邦治
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウイルスカプセルを中心とした金ナノ粒子の三次元配列構造体とその光学応答2009

    • Author(s)
      永川桂大、新倉謙一、大竹範子、鈴木忠樹、松尾保孝、澤洋文、居城邦治
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] JCウイルスの粒子形成機構の解析2009

    • Author(s)
      小林進太郎、鈴木忠樹、大竹範子、永川桂大、新倉謙一、木村享史、澤洋文
    • Organizer
      第57回日本ウイルス学会総会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] JCVウイルス Agnoprotein によるウイルス粒子の形態制御2009

    • Author(s)
      鈴木忠樹、仙葉慎吾、大場靖子、寸田祐嗣、木村享史、長嶋和郎、澤洋文
    • Organizer
      第57回日本ウイルス学会総会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] The human Polyoma JC virus acts as a viroporin2009

    • Author(s)
      鈴木忠樹、大場靖子、岡田由紀、寸田祐嗣、木村享史、田中伸哉、長嶋和郎、澤洋文
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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