Characterization of the molecular mechanism of Ebolavirus entry into host cells.

• Project/Area Number: 21890005
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Hokkaido University
• Principal Investigator: NANBO Asuka Hokkaido University, 大学院・薬学研究院, 講師 (60359487)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: エボラウイルス / 宿主因子 / エンドサイトーシス / 感染症

Research Abstract

We characterized the multiple host factors involved in actin reorganization play roles in the internalization of Ebolavirus viral particles. We also found that the internalization of viral particles was associated with actin-dependent membrane ruffling, suggesting that Ebolavirus particles are internalized in the identified host factors-dependent manner.

Research Products

• [Journal Article] Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. (2011)
  • Author(s): Ikeda O
  • Journal Title: Cancer Sci. Volume: 102(4) Pages: 756-761
  • Peer Reviewed
• [Journal Article] Zipper-interacting protein kinase modulates canonical Wnt/beta-catenin signaling through interacting with Nemo-like kinase and T-cell factor 4. (2011)
  • Author(s): Togi S
  • Journal Title: J Biol Chem. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. (2010)
  • Author(s): Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
  • Journal Title: Cancer Sci. 102巻 Pages: 756-761
  • NAID: 10029292209
  • Peer Reviewed
• [Journal Article] Functional involvement of Daxx in gp130-mediated cell growth and survival in BaF3 cells. (2010)
  • Author(s): Muromoto R, Kuroda M, Togi S, Sekine Y, Nanbo A, Shimoda K, Oritani K, Matsuda T
  • Journal Title: Eur J Immunol. 40巻 Pages: 3570-3580
  • NAID: 120002646892
  • Peer Reviewed
• [Journal Article] Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells. (2010)
  • Author(s): Ikeda O, Sekine Y, Mizushima A, Nakasuji M, Miyasaka Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
  • Journal Title: J Biol Chem. 285巻 Pages: 38093-38103
  • Peer Reviewed
• [Journal Article] Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner. (2010)
  • Author(s): Nanbo A, Imai M, Watanabe S, Noda T, Takahashi K, Neumann G, Halfmann P, Kawaoka Y
  • Journal Title: PLoS Pathogens.
  • NAID: 120004710991
  • Peer Reviewed
• [Journal Article] Ebolavirus is internalized into host cells via macropinocytosis in a viralglycoprotein-dependent manner. (2010)
  • Author(s): Nanbo A
  • Journal Title: PLoS Pathogens
  • NAID: 120004710991
  • Peer Reviewed
• [Journal Article] Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells. (2010)
  • Author(s): Ikeda O
  • Journal Title: J Biol Chem. Volume: 285(49) Pages: 38093-38103
  • Peer Reviewed
• [Journal Article] Functional involvement of Daxx in gp130-mediated cell growth and survival in BaF3 cells. (2010)
  • Author(s): Muromoto R
  • Journal Title: Eur J Immunol. Volume: 40(12) Pages: 3570-3580
  • Peer Reviewed
• [Journal Article] Signaltransducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells. (2009)
  • Author(s): Sekine Y, Ikeda O, Tsuji S, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
  • Journal Title: J.Immunol. 183巻 Pages: 7966-7974
  • Peer Reviewed
• [Journal Article] The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation. (2009)
  • Author(s): Muromoto R, Taira N, Ikeda O, Shiga K, Kamitani S, Togi S, Kawakami S, Sekine Y, Nanbo A, Oritani K, Matsuda T
  • Journal Title: Biochem.Biophys.Res.Commun. 382巻 Pages: 63-68
  • NAID: 120001164606
  • Peer Reviewed
• [Journal Article] HDAC3 influences phosphorylation of STAT3 at serine 727 by interacting with PP2A. (2009)
  • Author(s): Togi S, Kamitani S, Kawakami S, Ikeda O, Muromoto R, Nanbo A, Matsuda T
  • Journal Title: Biochem.Biophys.Res.Commun. 379巻 Pages: 616-620
  • NAID: 120001054060
  • Peer Reviewed
• [Journal Article] Mutational analysis of conserved amino acids in the influenza A virus nucleoprotein (2009)
  • Author(s): Li Z, Watanabe T, Hatta M, Watanabe S, Nanbo A, Ozawa M, Kakugawa S, Shimojima M, Yamada S, Neumann G, Kawaoka Y
  • Journal Title: J.Virol. 83巻 Pages: 4153-4162
  • Peer Reviewed
• [Journal Article] The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl. (2009)
  • Author(s): Sekine Y, Yamamoto C, Ikeda O, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
  • Journal Title: Biochem.Biophys.Res.Commun. 384巻 Pages: 187-192
  • NAID: 120001311780
  • Peer Reviewed
• [Journal Article] STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation (2009)
  • Author(s): Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T
  • Journal Title: Biochem.Biophys.Res.Commun. 384巻 Pages: 71-75
  • NAID: 120001311775
  • Peer Reviewed
• [Journal Article] BS69 negatively regulates the canonical NF-kB activation induced by Epstein-Barr virus-derived LMP1 (2009)
  • Author(s): Ikeda O, Sekine Y, Mizushima A, Oritani K, Yasui T, Fujimuro M, Muromoto R, Nanbo A, Matsuda T
  • Journal Title: FEBS Lett. 583巻 Pages: 1567-1574
  • Peer Reviewed
• [Journal Article] Silencing mediator of retinoic acid and thyroid hormone receptor regulates enhanced activation of signal transducer and activator of transcription 3 by epstein-barr virus-derived epstein-barr nuclear antigen 2 (2009)
  • Author(s): Ikeda O, Togi S, Kamitani S, Muromoto R, Sekine Y, Nanbo A, Fujimuro M, Matsuda T
  • Journal Title: Biol.Pharm.Bull. 32巻 Pages: 1283-1285
  • NAID: 130000117231
  • Peer Reviewed
• [Journal Article] Silencing mediator of retinoic acid and thyroid hormone receptor regulates enhanced activation of signal transducer and activator of transcription 3 by epstein-barr virus-derived epstein-barr nuclear antigen 2 (2009)
  • Author(s): Ikeda O
  • Journal Title: Biol.Pharm.Bull. 32 Pages: 1283-1285
  • Peer Reviewed
• [Journal Article] BS69 negatively regulates the canonical NF-kB activation induced by Ep stein-Barr virus-derived LMP1 (2009)
  • Author(s): Ikeda O
  • Journal Title: FEBS Lett. 583 Pages: 1567-1574
  • Peer Reviewed
• [Journal Article] STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation (2009)
  • Author(s): Ikeda O
  • Journal Title: Biochem.Biophys.Res.Commun. 384 Pages: 71-75
  • Peer Reviewed
• [Journal Article] The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl (2009)
  • Author(s): Sekine Y
  • Journal Title: Biochem.Biophys.Res.Commun. 384 Pages: 187-192
  • Peer Reviewed
• [Journal Article] Mutational analysis of conserved amino acids in the influenza A virus nucleoprotein (2009)
  • Author(s): Li Z
  • Journal Title: J.Virol. 83 Pages: 4153-4162
  • Peer Reviewed
• [Journal Article] HDAC3 influences phosphorylation of STAT3 at serine 727 by interacting with PP2A (2009)
  • Author(s): Togi S
  • Journal Title: Biochem.Biophys.Res.Commun. 379 Pages: 616-620
  • Peer Reviewed
• [Journal Article] The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation (2009)
  • Author(s): Muromoto R
  • Journal Title: Biochem.Biophys.Res.Commun. 382 Pages: 63-68
  • Peer Reviewed
• [Journal Article] Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells. (2009)
  • Author(s): Sekine Y
  • Journal Title: J.Immunol. 183 Pages: 7966-7974
  • Peer Reviewed
• [Journal Article] Zipper-interacting protein kinase modulates canonical Wnt/beta-catenin signaling through interacting with Nemo-like kinase and T-cell factor 4.
  • Author(s): Togi S, Ikeda O, Kamitani S, Nakasuji M, Sekine Y, Muromoto R, Nanbo A, Oritani K, Kawai T, Akira S, Matsuda T
  • Journal Title: J Biol Chem. (印刷中)
  • NAID: 120003005575
  • Peer Reviewed
• [Presentation] Ebolavirusの宿主細胞への侵入機構の解明 (2010)
  • Author(s): 南保明日香
  • Organizer: 遺伝子病制御研究所研究集会
  • Place of Presentation: 札幌市(招待講演)
  • Year and Date: 2010-12-22
• [Presentation] Ebolavirusの宿主細胞への侵入機構の解明 (2010)
  • Author(s): 南保明日香
  • Organizer: 遺伝子病制御研究所研究集会
  • Place of Presentation: 北海道札幌市 招待講演
  • Year and Date: 2010-12-22
• [Presentation] エボラウイルスの宿主細胞への侵入機構の解明 (2010)
  • Author(s): 南保明日香
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県徳島市
  • Year and Date: 2010-11-08
• [Presentation] エボラウイルスの宿主細胞への侵入機構の解明 (2010)
  • Author(s): 南保明日香
  • Organizer: 第10回蛋白質科学会年会
  • Place of Presentation: 札幌市(招待講演)
  • Year and Date: 2010-06-16
• [Presentation] エボラウイルスの宿主細胞への侵入機構の解明 (2010)
  • Author(s): 南保明日香
  • Organizer: 第10回蛋白質科学会年会
  • Place of Presentation: 北海道札幌市 招待講演
  • Year and Date: 2010-06-16
• [Presentation] Ebolavirus is internalized into host cells via macropinocytosis in a viralglycoprotein-dependent manner. (2010)
  • Author(s): 南保明日香
  • Organizer: 5th international Sympojsium of filoviruses.
  • Place of Presentation: 東京都白銀台
  • Year and Date: 2010-04-19
• [Presentation] Ebolavirus is internalized into host cells via macropinocytosis in a viraglycoprotein-dependent manner. (2010)
  • Author(s): Nanbo A
  • Organizer: 5^<th> international Sympojsium of filoviruses.
  • Place of Presentation: 東京都白銀台
  • Year and Date: 2010-04-19
• [Presentation] エボラウイルスの宿主細胞への侵入機構の解明 (2009)
  • Author(s): 南保明日香
  • Organizer: 生化学会北海道支部・生物物理学会北海道支部・北海道分子生物学研究会3支部合同シンポジウム
  • Place of Presentation: 札幌市
  • Year and Date: 2009-11-13
• [Presentation] エボラウイルスの宿主細胞への侵入機構の解明 (2009)
  • Author(s): 南保明日香
  • Organizer: 生化学会北海道支部・生物物理学会北海道支部・北海道分子生物学研究会 3支部合同シンポジウム
  • Place of Presentation: 札幌(北海道大学)
  • Year and Date: 2009-11-13
• [Remarks] ホームページ等
  • URL: http://www.hokudai.ac.jp/bureau/topics/press_release/101018_pr_pharm.pdf
• [Remarks] 北海道大学のホームページ上で研究成果が紹介された。
  • URL: http://www.hokudai.ac.jp/bureau/topics/press_release/101018_pr_pharm.pdf