Analysis of neural circuit formation in the cerebral cortex

• Project/Area Number: 21890046
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: The University of Tokyo
• Principal Investigator: AKINORI Tokunaga The University of Tokyo, 医科学研究所, 助教 (70549451)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: 神経発生 / 神経回路網 / 発生工学 / 神経回路綱 / 再生医療 / エレクトロポーレーション

Research Abstract

The molecular mechanism of regulating the patterning of neural circuits in developing brain had remained unclear. Therefore we introduced the GFP tracer into developing mouse brain by in utero electroporation to visualize cerebrocortical neuronal cells and the circuits. This method was useful for analyzing the candidate genes concerned with neuronal differentiation and the circuitry formation. By using this method, we identified several functional genes and generated conditional knockout mice.

Research Products

• [Journal Article] Fbx110/Kdm2b deficiency accelerates neural progenitor cell death and leads to exencephaly (2011)
  • Author(s): Fukuda T, Tokunaga A, Sakamoto R, Yoshida N.
  • Journal Title: Mol Cell Neurosci Volume: 46(3) Pages: 614-624
  • Peer Reviewed
• [Journal Article] BMP-induced REST regulates the establishment and maintenance of astrocytic identity. (2010)
  • Author(s): Kohyama J, Sanosaka T, Tokunaga A, Takatsuka E, Tsujimura K, Okano H, Nakashima K
  • Journal Title: J Cell Biol. 189(1) Pages: 159-170
  • NAID: 120005716808
  • Peer Reviewed
• [Journal Article] BMP-induced REST regulates the establishment and maintenance of astrocytic identity (2010)
  • Author(s): Kohyama J, Sanosaka T, Tokunaga A, Takatsuka E, Tsujimura K, Okano H, Nakashima K
  • Journal Title: J Cell Biol Volume: 189(1) Pages: 159-170
  • NAID: 120005716808
  • Peer Reviewed
• [Journal Article] Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation. (2009)
  • Author(s): Shibayama M, Ohno S, Osaka T, Sakamoto R, Tokunaga A, Nakatake Y, Sato M, Yoshida N
  • Journal Title: FEBS J. 276(22) Pages: 6658-6668
  • Peer Reviewed
• [Journal Article] Notch-Hes1 pathway contributes to the cochlear prosensory formation potentially through the transcriptional down-regulation of p27 (Kip1). (2009)
  • Author(s): Murata J, Ohtsuka T, Tokunaga A, Nishiike S, Inohara H, Okano H, Kageyama R
  • Journal Title: J Neurosci Res. 87(16) Pages: 3521-3534
  • Peer Reviewed
• [Journal Article] Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation. (2009)
  • Author(s): Shibayama M, Ohno S, Osaka T, Sakamoto R, Tokunaga A, Nakatake Y, Sato M, Yoshida N.
  • Journal Title: FEBS J. 276(22) Pages: 6658-6668
  • Peer Reviewed
• [Journal Article] Notch-Hesl pathway. contributes to the cochlear prosensory formation potentially through the transcriptional down-regulation of p27 (Kipl). (2009)
  • Author(s): Murata J, Ohtsuka T, Tokunaga A, Nishiike S, Inohara H, Okano H, Kageyama R.
  • Journal Title: J Neurosci Res. 87(16) Pages: 3521-3534
  • Peer Reviewed
• [Presentation] Targeted disruption of Fbxl10 causes neural tube defects resulting in exencephaly (2010)
  • Author(s): 福田剛、徳永暁憲、吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] PTB deficiency in the brain disrupts dorsal neuroepithelium of the lateral ventricles and leads lethal hydrocephalus (2010)
  • Author(s): 柴嵜孝幸、徳永暁憲、吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Targeted disruption of Fbxl10 causes neural tube defects resulting in exencephaly (2010)
  • Author(s): 福田剛、徳永暁憲, 吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] PTB deficiency in the brain disrupts dorsal neuroepithelium of the lateral ventricles and leads lethal hydrocephalus (2010)
  • Author(s): 柴嵜孝幸, 徳永暁憲, 吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸・神戸ポートアインド
  • Year and Date: 2010-12-08
• [Presentation] Functional analysis of nPTB, nervous system specific RNA binding protein (2010)
  • Author(s): 正木信也、徳永暁憲、吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Functional analysls of nPTB, nervous system specific RNA binding protein (2010)
  • Author(s): 正木信也, 徳永暁憲, 吉田進昭
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会大会
  • Place of Presentation: 神戸・神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Polypyrimidine tract binding protein regulates alternative splicing of Numb exon9 (2009)
  • Author(s): 柴嵜孝幸、徳永暁憲、吉田進昭
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Polypyrimidine tract binding protein regulates alternative splicing of Numb exon 9 (2009)
  • Author(s): 柴嵜孝幸, 徳永暁憲, 吉田進昭
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜・日本
  • Year and Date: 2009-12-11
• [Remarks] ホームページ
  • URL: http://www.ims.u-tokyo.ac.jp/cem_ger/HPidenshikinou/main.html
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/cem_ger/HPidenshikinou/main.html