Vascularized Nerve Flap for Spinal Cord Repair
| Project/Area Number |
21890060
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIKUCHI Kazuki The University of Tokyo, 医学部附属病院, 特任臨床医 (40508090)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Keywords | 脊髄損傷 / マイクロサージャリー / 血管柄付き神経移植 / 脊髄再生 / 中枢神経再生 / マクロサージャリー / 再生医療 / 脊髄移植 |
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| Research Abstract |
Object : By making the "vascularized nerve flap for complete spinal cord gap" model, we assessed the histological effect of this method. Method : Eight female Sprague-Dawley rats were divided into 2 groups : group A and group B. Group A included "nonvascularized nerve flap" models, whereas group B consisted of "vascularized nerve flap" models. In each group, we transferred peripheral nerves to fill the void made by complete surgical transection of the spinal cord. Postoperatively, we administered intravenous antibiotics and performed urethral catheterization and disimpaction everyday. Tissue sampling was done on postoperative day 10. Results : No necrosis occurred in group B, vascularized models. In group A, nonvascularized models, necrosis of the grafted nerves was observed in 2 of the 4 rats. As for the specimens of Kluver-Barrera's method for selective myelin sheath staining, in group B, all specimens were stained with Kluver-Barrera's method for selective myelin sheath staining, whereas in group A, 2 specimens with successful engraftment were not stained. With type IV collagen immunostaining, the increase of type IV collagen around the grafted area was not so severe, compared with that of normal region in vascularized cases. In nonvascularized cases, the appearance of type IV collagen was prominent. Therefore, in the vascularized cases, it was made clear that Schwann cells survived in vascularized models and that fibrotic scar formation was inhibited. Conclusion : Histological comparison between vascularized and nonvascularized peripheral nerve transfer for complete spinal cord gap showed significant differences in 10 days. A longer follow-up period is necessary for observation of functional differences.
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Report
(3 results)
Research Products
(22 results)
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[Presentation]2010
Organizer
第37回日本マイクロサージャリー学会学術集会
Place of Presentation
愛知県産業労働センター
Year and Date
2010-11-18
Related Report
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[Presentation]2010
Organizer
第45回日本脊髄障害医学会
Place of Presentation
長野県松本文化会館
Year and Date
2010-10-22
Related Report
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[Presentation]2010
Organizer
第19回日本形成外科学会基礎学術集会
Place of Presentation
パシフィコ横浜
Year and Date
2010-09-16
Related Report
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[Presentation]2010
Organizer
6^<th> International Muscle Symposium
Place of Presentation
The University of Vienna, Wien, Austria
Year and Date
2010-09-02
Related Report
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[Presentation]2010
Organizer
第53回日本形成外科学会総会
Place of Presentation
ANAクラウンプラザホテル金沢
Year and Date
2010-04-07
Related Report
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[Presentation]2010
Organizer
1^<st> European Meeting on Supra microsurgery
Place of Presentation
Sant Pau Hospital, Barcelona, Spain
Year and Date
2010-03-05
Related Report
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[Presentation]2009
Organizer
第44回日本脊髄障害医学会
Place of Presentation
東京国際フォーラム
Year and Date
2009-11-12
Related Report
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