| Project/Area Number |
21890095
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
OZATO Nami Nagoya University, 医学系研究科, COE特任助教 (60547454)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Keywords | 脂肪滴 / 凍結割断レプリカ標識法 / (位相差)電子顕微鏡 / ADRP / 小胞体膜ドメイン / 凍結割断レプリカ法 / 小胞体 / 電子顕微鏡 / 脂防滴 |
|---|
| Research Abstract |
In this study, as the aim to clear the function of lipid droplets, I noticed the structure and formation of lipid droplets. I performed the biochemical experiments and the analysis of ultra-structure by using freeze-fracture replica staining and ultra-section with the electron microscope observation. In the biochemical experiments, it revealed that the lipid droplets from culture cells had many proteins, which were distributed in the endoplasmic reticulum. In the electron microscope observations, there were differences in the structures between artificial lipid droplets, made to contain the same elements, and the prepared lipid droplets from the culture cells.
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