Reveal the mechanism of neocortical development by live imaging

• Project/Area Number: 21890096
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Nagoya University
• Principal Investigator: SAKAKIBARA Akira Nagoya University, 医学系研究科, 助教 (20510217)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: 大脳皮質形成 / 細胞移動 / 細胞極性 / 細胞骨格 / イメージング / 微小管 / 中心体 / ゴルジ装置 / 軸索 / 樹状突起 / ニューロン移動 / 軸索形成 / 樹状突起形成 / 神経発生

Research Abstract

In the present study, we have highlighted some critical points regarding the centrosome positioning in neocortical pyramidal neurons during the migration and axon/dendrite formation in situ. Major points are : 1) centrosome in migrating multipolar neurons moves toward the most dominantly growing process ; 2 leading centrosome is occasionally overtaken by rapidly translocating nucleus during a locomotion cycle ; 3) centrosome proximity appears not to be essential to the backward formation of an axon-like process by locomoting neurons.

Research Products

• [Journal Article] early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior born Purkinje cells in the developing mouse lateral cerebellum. (2010)
  • Author(s): Miyata, T., Ono, Y., Okamoto, M., Masaoka, M., Sakakibara A., Kawaguchi, A., Hashimoto, M., Ogawa, M., Migration
  • Journal Title: Neural Dev. 5 Pages: 23-23
• [Journal Article] Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis (2010)
  • Author(s): Wang, Y., Nakayama, M., Pitulescu, M.E., Schmidt, T.S., Bochenek, M.L., Sakakibara A., Adams, S., Davy, A., Deutsch, U., Barberis, A., Benjamin, L. E., Makinen, T., Nobes, C.D., Adams, R.H.
  • Journal Title: Nature 465 Pages: 483-486
• [Journal Article] Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis (2010)
  • Author(s): Wang, Y., Nakayama, M., Pitulescu, M.E., Schmidt, T.S., Bochenek, M.L., Sakakibara, A., Adams, S., Davy, A., Deutsch, U., Barberis, A., Benjamin, L.E., Makinen, T., Nobes, C.D., Adams, R.H.
  • Journal Title: Nature Volume: 465 Pages: 483-486
  • Peer Reviewed
• [Journal Article] Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior born Purkinje cells in the developing mouse lateral cerebellum (2010)
  • Author(s): Miyata, T., Ono, Y., Okamoto, M., Masaoka, M., Sakakibara, A., Kawaguchi, A., Hashimoto, M., Ogawa, M.
  • Journal Title: Neural Dev. Volume: 5 Pages: 23-23
  • Peer Reviewed
• [Journal Article] Rac is involved in the interkinetic nuclear migration of cortical progenitor cells. (2009)
  • Author(s): Minobe, S., Sakakibara A., Ohdachi, T., Kanda, R., Kimura, M., Nakatani, S., Tadokoro, R., Ochiai, W., Nishizawa, Y., Mizoguchi, A., Kawauchi, T., Miyata, T.
  • Journal Title: Neurosci.Res. 63 Pages: 294-301
• [Journal Article] Rac is involved in the interkinetic nuclear migration of cortical progenitor cells (2009)
  • Author(s): Sayaka Minobe
  • Journal Title: Neurosci. Res. 63 Pages: 294-301
  • Peer Reviewed
• [Presentation] Function of microtubule cytoskeleton in polarization of neocortical pyramidal neurons during the 3D migration and axon/dendrite formation in the developing mouse cerebrum. (2010)
  • Author(s): 榊原明, 佐藤俊之, 正岡実, 宮田卓樹
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center (USA)
  • Year and Date: 2010-12-14
• [Presentation] Function of microtubule cytoskeleton in polarization of neocortical pyramidalneurons during the 3D migration and axon/dendrite formation in the developingmouse cerebrum (2010)
  • Author(s): Sakakibara, A., Sato, T., Masaoka, M., Miyata, T.
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center (USA)
  • Year and Date: 2010-12-14
• [Presentation] Dynamics of centrosome and Golgi apparatus in neocortical pyramidal neurons during the transition of migration mode from multipolar to locomotion, and formation of axon-like fiber. (2010)
  • Author(s): 榊原明, 佐藤俊之, 正岡実, 小川正晴, 宮田卓樹
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2010-11-14
• [Presentation] Dynamics of centrosome and Golgi appazatus in neocortical pyramidal neurons during the transition of migration mode from multipolar to locomotion, and formation of axon-like fiber (2010)
  • Author(s): Sakakibara, A., Sato, T., Masaoka, M., Ogawa, M., Miyata, T.
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego Convention Center(USA)
  • Year and Date: 2010-11-14
• [Presentation] 大脳皮質錐体ニューロンの示す多極型からロコモーションへの移動モード転換と軸索様突起伸長開始過程における中心体、ゴルジ装置の細胞内動態 (2010)
  • Author(s): 榊原明、佐藤俊之、正岡実、小川正晴、宮田卓樹
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] 大脳皮質錐体ニューロンの示す多極型からロコモーションへの移動モード変換と軸索様突起伸長開始過程における中心体、ゴルジ装置の細胞内動態 (2010)
  • Author(s): 榊原明、佐藤俊之、正岡実、小川正晴、宮田卓樹
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] 胎生期リーラー大脳皮質における異常な向きのニューロン移動 (2010)
  • Author(s): 佐藤俊之、榊原明、正岡実、小川正晴、宮田卓樹
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] 皮質板ニューロンに形成される一次樹状突起の形態的起源と動的変化:大脳皮質形成の実態 (2010)
  • Author(s): 榊原明、佐藤俊之、正岡実、小川正晴、宮田卓樹
  • Organizer: 第43回発生生物学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2010-06-22
• [Presentation] マウス胎仔大脳皮質スライス培養を利用した錐体ニューロンの移動と軸索、樹状突起形成の動態解析 (2010)
  • Author(s): 榊原明、佐藤俊之、正岡実、小川正晴、宮田卓樹
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館(岩手県)
  • Year and Date: 2010-03-28
• [Presentation] Live imaging the migration, axon/dendrite formation, and MTOC dynamics of cortical plate neurons under the 3D slice culture of embryonic mouse cerebrum. (2009)
  • Author(s): 榊原明, 佐藤俊之, 正岡実, 小川正晴, 宮田卓樹
  • Organizer: The 49th American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2009-12-07
• [Presentation] Live imaging the migration, axon/dendrite formation, and MTOC dynamics of cortical plate neurons under the 3D slice culture of embryonic mouse cerebrum (2009)
  • Author(s): Akira Sakakibara
  • Organizer: The 49th American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Diego Convention Center(USA)
  • Year and Date: 2009-12-07
• [Presentation] Observation of cellular behaviors that form the Purkinje plate in the embryonic cerebellum (2009)
  • Author(s): Takaki Miyata
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: McCormick Place(Chicago, USA)
  • Year and Date: 2009-10-19
• [Presentation] ライブイメージングに基づくマウス大脳皮質層形成過程の解析 (2009)
  • Author(s): 榊原明、佐藤俊之、正岡実、小川正晴、宮田卓樹
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-18
• [Presentation] Live imaging the cortical lamination in slice culture of embryonic mouse cerebrum. (2009)
  • Author(s): 榊原明, 佐藤俊之, 正岡実, 小川正晴, 宮田卓樹
  • Organizer: The 16th International Society of Developmental Biologists Congre ss
  • Place of Presentation: Edinburgh International Conference Centre (UK)
  • Year and Date: 2009-09-08
• [Presentation] Live imaging the cortical lamination in slice culture of embryonic mouse cerebrum (2009)
  • Author(s): Akira Sakakibara
  • Organizer: The 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Edinburgh International Conference Centre(UK)
  • Year and Date: 2009-09-08
• [Presentation] マウス大脳皮質ニューロンの移動と層形成機構のin situイメージング解析 (2009)
  • Author(s): 榊原明、佐藤俊之、正岡実、宮田卓樹
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-06-03
• [Presentation] 大脳皮質層形成過程におけるニューロン移動動態と細胞内MTOC局在の観察 (2009)
  • Author(s): 佐藤俊之
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-06-03
• [Presentation] マウス胎仔大脳皮質スライス培養を利用した錐体ニューロンの移動と軸索、樹状突起形成の動態解析 (2009)
  • Author(s): 榊原明
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館(岩手県)
  • Year and Date: 2009-03-28
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/dev-bio/staff/sakakibara.html