Developing a novel therapy with umbilical cord blood cells for perinatal brain injuries

• Project/Area Number: 21890100
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Nagoya University
• Principal Investigator: SATO Yoshiaki Nagoya University, 医学部附属病院, 助教 (30435862)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,535,000 (Direct Cost: ¥1,950,000、Indirect Cost: ¥585,000); Fiscal Year 2010: ¥1,196,000 (Direct Cost: ¥920,000、Indirect Cost: ¥276,000); Fiscal Year 2009: ¥1,339,000 (Direct Cost: ¥1,030,000、Indirect Cost: ¥309,000)
• Keywords: 臍帯血 / 周産期脳障害 / 臍帯血幹細胞

Research Abstract

It is urgent to develop novel therapies for infants with perinatal hypoxic-ischemic encephalopathy (HIE). In the present project, we have performed basic experiments for developing a novel therapy with human umbilical cord blood cells (HUCB), which is most ideal source of stem cells for perinatal brain injuries. After establishing a perinatal HI rat model, we evaluated the effect of administration of HUCB on perinatal HIE. We injected HUSB peritoneally after hypoxic-ischemic insult. The injected HUSB migrated into the brain, and tended to exert the anti-apoptotic and anti-oxidative effect, and increase the cell proliferation.

Research Products

• [Journal Article] Carbohydrate and Energy Metabolism in the Brain of Rats with Thromboxane A2-Induced Fetal Growth Restriction. (2011)
  • Author(s): Hayakawa M, Sato Y, Hattori T, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Miharu I, Ieda K, Kojima S.
  • Journal Title: Pediatr Res in press
  • Peer Reviewed
• [Journal Article] Carbohydrate and Energy Metabolism in the Brain of Rats with Thromboxane A2 -Induced Fetal Growth Restriction. (2011)
  • Author(s): Hayakawa M, Sato Y, Hattori T, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Miharu I, Ieda K, Kojima S
  • Journal Title: Pediatr Res Volume: in press
  • Peer Reviewed
• [Journal Article] The diffusion weighted imaging for an early diagnosis of parasagittal injury. (2010)
  • Author(s): Tagaya M, Sato Y, Hayakawa M
  • Journal Title: Pediatr Int 52(2) Pages: 298-301
  • NAID: 10028182201
  • Peer Reviewed
• [Journal Article] Apoptosis-inducing factor deficiency decreases the proliferation rate and protects the subventricular zone against ionizing radiation. (2010)
  • Author(s): Osato K*, Sato Y(*equally contribution), Ochiishi T, Osato A, Zhu C, Sato M, Swanpalmer J
  • Journal Title: Cell Death & Disease 1
  • Peer Reviewed
• [Journal Article] Biological activities of highly sulfated chondroitin sulfate polysaccharides on neural cells. (2010)
  • Author(s): Nakanishi K, Sato Y, Oohira A.
  • Journal Title: Research Advances in Neurochemistry, Global Research Network 1 Pages: 1-10
• [Journal Article] The diffusion weighted imaging for an early diagnosis of parasagittal injury. (2010)
  • Author(s): Tagaya M, Sato Y, Hayakawa M.
  • Journal Title: Pediatr Int Volume: 52 Pages: 298-301
  • NAID: 10028182201
  • Peer Reviewed
• [Journal Article] Apoptosis-inducing factor deficiency decreases the proliferation rate and protects the subventricular zone against ionizing radiation. (2010)
  • Author(s): Osato K^*, Sato Y^*(^*equally contribution), Ochiishi T, Osato A, Zhu C, Sato M, Swanpalmer J, Modjtahedi N, Kroemer G, Kuhn HG, Blomgren K.
  • Journal Title: Cell Death & Disease Volume: 1
  • Peer Reviewed
• [Journal Article] Biological activities of highly sulfated chondroitin sulfate polysac charides on neural cells. (2010)
  • Author(s): Nakanishi K, Sato Y, Oohira A.
  • Journal Title: Research Advances in Neurochemistry, Global Research Network 2 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate, a major niche substance of neuralstem cells, and cell transplantation therapy of neurodegeneration combined with niche modification. (2009)
  • Author(s): Sato Y, Oohira A.
  • Journal Title: Curr Stem Cell Res Ther 4(3) Pages: 200-209
  • Peer Reviewed
• [Journal Article] Male gender is related to the development of parenteral nutrition-associated cholestasis in neonates. (2009)
  • Author(s): Takemoto K, Nakayama A, Ito M, Sato Y, Saito A, Tori Y, Kaneko K, Ando H, Hayakawa M
  • Journal Title: Journal of Neonatal-Perinatal Medicine 2(4) Pages: 247-251
  • Peer Reviewed
• [Journal Article] Behavioral abnormalities of fetal growth retardation model rats with reduced amounts of brain proteoglycans. (2009)
  • Author(s): Saito A, Matsui F, Hayashi K, Watanabe K, Ichinohashi Y, Sato Y, Hayakawa M, Kojima S, Oohira A.
  • Journal Title: Exp Neurol 219(1) Pages: 81-92
  • Peer Reviewed
• [Journal Article] 早産重症胎児発育不全severe preterm FGR(IUGR)病因と病態 胎児発育不全モデルラットの中枢神経における組織学的および分子生物学的変化に関する研究 (2009)
  • Author(s): 早川昌弘, 中山淳, 竹本康二, 伊東真隆, 齊藤明子, 佐藤義朗
  • Journal Title: 周産期学シンポジウム 09(27) Pages: 37-42
• [Journal Article] 【新生児の薬物療法】各疾患への薬物療法 新生児痙攣に対するフェノバルビタール療法 (2009)
  • Author(s): 佐藤義朗, 早川昌弘
  • Journal Title: 周産期医学 39(12) Pages: 1687-1690
• [Journal Article] 新生児代表的疾患の基礎理解(第5回)脳室内出血(IVH) (2009)
  • Author(s): 佐藤義朗
  • Journal Title: こどもケア 4(5) Pages: 85-91
• [Journal Article] Male gender is related to the development of parenteral nutrition-associated cholestasis in neonates. (2009)
  • Author(s): Takemoto K, Nakayama A, Ito M, Sato Y, Saito A, Tori Y, Kaneko K, Ando H, Hayakawa M.
  • Journal Title: Journal of Neonatal-Perinatal Medicine 4 Pages: 247-251
  • Peer Reviewed
• [Journal Article] Chondroitin Sulfate, a Major Niche Substance of Neural Stem Cells, and Cell Transplantation Therapy of Neurodegeneration Combined with Niche Modification. (2009)
  • Author(s): Sato Y, Oohira A.
  • Journal Title: Curr Stem Cell Res Ther 4 Pages: 200-209
  • Peer Reviewed
• [Journal Article] Behavioral abnormalities of fetal growth retardation model rats with reduced amounts of brain proteoglycans. (2009)
  • Author(s): Saito A, Matsui F, Hayashi K, Watanabe K, Ichinohashi Y, Sato Y, Hayakawa M, Kojima S, Oohira A.
  • Journal Title: Curr Stem Cell Res Ther 219 Pages: 81-92
  • Peer Reviewed
• [Presentation] 未熟児網膜症に対する光凝固術施行時の鎮痛・鎮静 (2011)
  • Author(s): 佐藤義朗, 大城誠, 竹本康二, 細野治樹, 齊藤明子, 近藤大貴, 会津研二, 松沢麻衣子, 寺崎浩子, 早川昌弘
  • Organizer: 第29回周産期学シンポジウム
  • Place of Presentation: 佐賀
• [Presentation] 未熟児網膜症に対する光凝固術施行時の鎮痛・鎮静 (2011)
  • Author(s): 佐藤義朗,_大城誠, 竹本康二, 細野治樹, 齊藤明子, 近藤大貴, 会津研二, 松沢麻衣子, 寺崎浩子, 早川昌弘
  • Organizer: 第29回周産期学シンポジウム
  • Place of Presentation: 佐賀、どんぐり村"森のコンサートホール"
• [Presentation] 小児脳腫瘍における頭部放射線療法後の脳障害克服を目指して (2010)
  • Author(s): 佐藤義朗
  • Organizer: 第562回九州大学生医研セミナー(多階層生体防御システム研究拠点)グローバルCOE理医連携セミナー(招待講演)
  • Place of Presentation: 福岡
  • Year and Date: 2010-12-02
• [Presentation] 小児脳腫瘍における頭部放射線療法後の脳障害克服を目指して (2010)
  • Author(s): 佐藤義朗
  • Organizer: 第562回九州大学生医研セミナー(多階層生体防御システム研究拠点)グローバルCOE理医連携セミナー
  • Place of Presentation: 福岡、九州大学
  • Year and Date: 2010-12-02
• [Presentation] Carbohydrate and Energy Metabolism in the Brain of Rats with Thromboxane (2010)
  • Author(s): Hayakawa M, Sato Y, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Miharu I, Ieda K, Kojima.
  • Organizer: A2 -Induced Fetal Growth RestrictionHot topics in Neonatology 2010
  • Place of Presentation: Washington DC, USA
• [Presentation] 未熟児無呼吸治療/予防薬の神経幹細胞に対する細胞毒性および増殖に及ぼす影響 (2010)
  • Author(s): 佐藤義朗, 中西圭子, 伊藤美春, 一ノ橋祐子, 服部哲夫, 邊見勇人, 中山淳, 山本ひかる, 早川昌弘
  • Organizer: 第55回日本未熟児新生児学会・学術集会
  • Place of Presentation: 神戸
• [Presentation] ステロイドによる神経幹細胞の増殖と分化への影響 (2010)
  • Author(s): 一ノ橋祐子、佐藤義朗、服部哲夫、伊藤美春、山本ひかる、中山淳、早川昌弘
  • Organizer: 第55回日本未熟児新生児学会・学術集会
  • Place of Presentation: 神戸
• [Presentation] Apoptosis-inducing factor deficiency decreases the proliferation rate of neural precursor cells and protects the subventricular zone against ionizing radiation (2010)
  • Author(s): Sato Y, Osato K, Ochiishi T, Osato A, Zhu C, Sato M, Swanpalmer J, Modjtahedi N, Kroemer G, Kuhn GH, Blomgren K
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Cerebral Maturation in Preterm SGA Infants (2010)
  • Author(s): Sato Y, Hattori T, Suzuki T, Hayashi N, Ito M, Hemmi H, Nakayama A, Yamamoto H, Ichinohashi Y, Hayakawa M
  • Organizer: 第46回日本周産期・新生児医学会学術集会
  • Place of Presentation: 神戸
• [Presentation] A simple scoring system using fetal MRI for prognosis in left congenital diaphragmatic hernia (2010)
  • Author(s): Hattori T, Sato Y, Itoh M, Tsuda H, Hemmi H, Sumigama S, Yamamoto H, Nakayama A, Kotani T, Hayakawa H, Hayakawa M
  • Organizer: 第46回日本周産期・新生児医学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 中枢神経疾患に対する幹細胞療法 ～周産期脳障害の救世主となりうるか～ (2010)
  • Author(s): 佐藤義朗, 服部哲夫, 中西圭子, 大平敦彦, Klas Blomgren, 早川昌弘
  • Organizer: 第52回日本小児神経学会総会(シンポジウム)
  • Place of Presentation: 福岡
• [Presentation] Carbohydrate and Energy Metabolism in the Brain of Rats with Thromboxane A2 -Induced Fetal Growth Restriction (2010)
  • Author(s): Hayakawa, M, Sato Y, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Miharu I, Ieda K, Kojima.
  • Organizer: Hot topics in Neonatology 2010
  • Place of Presentation: Omni Shoreham Hotel, Washington DC, USA
• [Presentation] 未熟児無呼吸治療/予防薬の神経幹細胞に対する細胞毒性および増殖に及ぼす影響 (2010)
  • Author(s): 佐藤義朗, 中西圭子, 伊藤美春, 一ノ橋祐子, 服部哲夫, 邊見勇人, 中山淳, 山本ひかる, 早川昌弘
  • Organizer: 第55回日本未熟児新生児学会・学術集会
  • Place of Presentation: 神戸、神戸国際会議場
• [Presentation] Apoptosis-inducing factor deficiency decreases the proliferation rate of neural precursor cells and protects the subventricular zone against ionizing radiation (2010)
  • Author(s): Sato Y, Osato K, Ochiishi T, Osato A, Zhu C, Sato M, Swanpalmer J, Modjtahedi N, Kroemer G, Kuhn GH, Blomgren K
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸、神戸国際会議場
• [Presentation] Cerebral Maturation in Preterm SGA Infants (2010)
  • Author(s): Sato Y, Hattori T, Suzuki T, Hayashi N, Ito M, Hemmi H, Nakayama A, Yamamoto H, Ichinohashi Y, Hayakawa M
  • Organizer: 第46回日本周産期・新生児医学会学術集会
  • Place of Presentation: 神戸、神戸国際会議場
• [Presentation] 中枢神経疾患に対する幹細胞療法 ～周産期脳障害の救世主となりうるか～ (2010)
  • Author(s): 佐藤義朗, 服部哲夫, 中西圭子, 大平敦彦, Klas Blomgren, 早川昌弘
  • Organizer: 第52回日本小児神経学会総会(シンポジウム)
  • Place of Presentation: 福岡、福岡国際会議場
• [Presentation] 在胎22・23週児における中枢神経受傷機序の解明 ～経時的脳波を用いた検討～ (2009)
  • Author(s): 佐藤義朗、松沢麻衣子、邊見勇人、山本ひかる、中山淳、早川昌弘
  • Organizer: 第54回日本未熟児新生児学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-11-30
• [Presentation] Serial EEG findings in very premature infants born at gestational age of 22-23 weeks (2009)
  • Author(s): Hayakawa M., Sato Y., Nakayama A., Henmi H., Yamamoto H., Hattori T., Hayashi S., Tsuji T., Kato T., Hayakawa F., Kato Y., Takemoto K., Matsuzawa K., Kidokor H., Kubota T., Oshiro M
  • Organizer: Hot Topics in Neonatology 2009
  • Place of Presentation: Washington DC
• [Presentation] 在胎22・23週児における中枢神経受傷機序の解明 ～経時的脳波を用いた検討～ (2009)
  • Author(s): 佐藤義朗、服部哲夫、大城誠、加藤有一、城所博之、久保田哲夫、林誠司、加藤徹、松沢麻衣子、邊見勇人、山本ひかる、中山淳、早川昌弘
  • Organizer: 第54回日本未熟児新生児学会・学術集会(シンポジウム)
  • Place of Presentation: 横浜
• [Presentation] Neural stem/progenitor cells transplanted into the hippocampus of young mice survive and normalize behavior changes induced by irradiation (2009)
  • Author(s): Sato Y, Shinjyo N, Karlsson N, Nilsson M, Sato M, Oosato K, Zhu C, Kuhn HG, Pekny M, Pekna M, Blomgren K
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 未熟児無呼吸治療/予防薬の神経幹細胞への影響 (2009)
  • Author(s): 佐藤義朗、中西圭子、早川昌弘
  • Organizer: 第45回日本周産期・新生児医学会学術集会
  • Place of Presentation: 名古屋
• [Remarks] ホームページ等