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ストレス性自律生理反応の神経科学的基盤

Research Project

Project/Area Number 21890114
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKyoto University

Principal Investigator

中村 和弘  京都大学, 医学(系)研究科(研究院), 助教 (00548521)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,224,000 (Direct Cost: ¥2,480,000、Indirect Cost: ¥744,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,664,000 (Direct Cost: ¥1,280,000、Indirect Cost: ¥384,000)
Keywordsストレス / 高体温症 / 光遺伝学 / 情動 / 脳神経回路 / ウイルス / 自律神経 / ホメオスタシス
Research Abstract

情動やストレス刺激は、自律神経系を含めた生体の恒常性維持機能に強い影響を与えるが、その脳内メカニズムはほとんど分かっていない。本研究では、情動を司る大脳辺縁系から恒常性維持機能を司る視床下部や脳幹への情動シグナル伝達に焦点を当て、その神経メカニズムの解明を目指す。そして、その目的のために、神経活動の光遺伝学的操作技術をin vivoの統合生理学に応用し、脳内の特定部位に投射するニューロン群の活動を特異的に操作する新規技術を開発する。
平成21年度は、光によって活性化するカチオンチャネル(ChR2)およびクロライドポンプ(eNpHR)の遺伝子を組み込んだウイルスベクターを作製し、そのウイルスをラットの体温調節に関わる視床下部や脳幹の領域に注入して、その領域のニューロン群が感染することを組織学的に同定することに成功した。次年度は、このウイルスを用いて、覚醒ラットの大脳辺縁系の様々な部位から恒常性維持機能に関わる脳部位への神経接続を特異的に活性化、あるいは抑制し、そのストレス性(情動性)自律神経反応への影響を調べることで、情動や心理ストレスが生体の恒常性維持機能に作用する神経科学的メカニズムを明らかにする。
本研究によって得られる成果は、自律神経失調症などの様々なストレス疾患の病態解明に貢献するものであり、また、本研究で開発する神経接続の特異的操作技術は、多様な脳機能を司る神経回路及びその動作原理の解明に幅広く応用しうるものである。

Report

(1 results)
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article]2010

    • Author(s)
      Garami, et al.
    • Journal Title

      章題 : The TRPV1 channel in normal thermoregulation : What have we learned from experiments using different tools? 書名 : Vanilloid Receptor TRPV1 in Drug Discovery : Targeting Pain and Other Pathological Disorders(John Wiley & Sons)

      Pages: 351-402

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Different populations of prostaglandin EP3 receptor-expressing preoptic neurons project to two fever-mediating sympathoexcitatory brain regions2009

    • Author(s)
      Nakamura, et al.
    • Journal Title

      Neuroscience 161

      Pages: 614-620

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vanilloid channel TRPV1 in thermoregulation : a thermosensor it is not2009

    • Author(s)
      Romanovsky, et al.
    • Journal Title

      Pharmacological Reviews 61

      Pages: 228-261

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neutralization of granulocyte macrophage colony-stimulating factor decreases amyloid beta 1-42 and suppresses microglial activity in a transgenic mouse model of Alzheimer's disease2009

    • Author(s)
      Manczak, et al.
    • Journal Title

      Human Molecular Genetics 18

      Pages: 3876-3893

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Granulocyte-macrophage colony-stimulating factor antibody suppresses microglial activity : implications for anti-inflammatory effects in Alzheimer's disease and multiple sclerosis2009

    • Author(s)
      Reddy, et al.
    • Journal Title

      Journal of Neurochemistry 111

      Pages: 1514-1528

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 体温調節のメカニズム2009

    • Author(s)
      中村和弘
    • Organizer
      第62回日本自律神経学会総会
    • Place of Presentation
      ダイワロイネットホテル和歌山 (和歌山市)
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 対暑反応に必要な温度感覚の伝達を担う外側結合腕傍核ニューロン2009

    • Author(s)
      中村和弘, Shaun F Morrison
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場 (名古屋市)
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Neural circuitry to defend body temperature from environmental thermal challenge2009

    • Author(s)
      中村和弘, Morrison SF
    • Organizer
      Autonomic Adjustments to Environmental Challenges-A Satellite Meeting of ISAN 2009
    • Place of Presentation
      ニューカッスル (オーストラリア)
    • Year and Date
      2009-09-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] The ways up and the ways down : dissecting neural pathways for thermoregulation2009

    • Author(s)
      中村和弘, et al.
    • Organizer
      6th Congress of the International Society for Autonomic Neuroscience (ISAN 2009)
    • Place of Presentation
      マンリー (オーストラリア)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Different populations of prostaglandin EP3 receptor-expressing ratpreoptic neurons project to two fever-mediating sympathoexcitatory brain regions2009

    • Author(s)
      Nakamura Y, et al.
    • Organizer
      6th Congress of the International Society for Autonomic Neuroscience (ISAN 2009)
    • Place of Presentation
      マンリー (オーストラリア)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 体温調節の中枢メカニズム2009

    • Author(s)
      中村和弘
    • Organizer
      日本麻酔科学会第56回学術集会
    • Place of Presentation
      神戸ポートピアホテル (神戸市)
    • Year and Date
      2009-08-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Differential control of thermal effector pathways contributing to fever2009

    • Author(s)
      中村和弘, et al.
    • Organizer
      36th International Congress of Physiological Sciences (IUPS2009)
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Lateral parabrachial neurons that mediate the thermosensory afferent signaling required for heat defense2009

    • Author(s)
      中村和弘, Morrison SF
    • Organizer
      36th International Congress of Physiological Sciences (IUPS2009)
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Double tracing of prostaglandin EP3 receptor-expressing preoptic neurons innervating dorsomedial hypothalamus and rostral raphe pallidus2009

    • Author(s)
      Nakamura Y, et al.
    • Organizer
      36th International Congress of Physiological Sciences (IUPS2009)
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Spinal serotonergic neurotransmission contributes to EMG activation during skin cooling-evoked shivering2009

    • Author(s)
      Morrison SF, 中村和弘
    • Organizer
      36th International Congress of Physiological Sciences (IUPS2009)
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Thermosensory ascending pathways leading to body temperature control2009

    • Author(s)
      中村和弘, Morrison SF
    • Organizer
      The 3rd International Symposium on Physiology and Pharmacology of Temperature Regulation (PPTR2009)
    • Place of Presentation
      くにびきメッセ (松江市)
    • Year and Date
      2009-07-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Central efferent system for thermoregulation and energy expenditure2009

    • Author(s)
      Morrison SF, 中村和弘
    • Organizer
      The 3rd International Symposium on Physiology and Pharmacology of Temperature Regulation (PPTR2009)
    • Place of Presentation
      くにびきメッセ (松江市)
    • Year and Date
      2009-07-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Lateral parabrachial neurons that mediate the thermosensory afferent signaling required for heat defense2009

    • Author(s)
      中村和弘, Morrison SF
    • Organizer
      The 3rd International Symposium on Physiology and Pharmacology of Temperature Regulation (PPTR2009)
    • Place of Presentation
      くにびきメッセ (松江市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Double tracing analysis of prostaglandin EP3 receptor-expressing preoptic neurons that project to the dorsomedial hypothalamus and rostral raphe pallidus2009

    • Author(s)
      Nakamura Y, et al.
    • Organizer
      The 3rd International Symposium on Physiology and Pharmacology of Temperature Regulation (PPTR2009)
    • Place of Presentation
      くにびきメッセ (松江市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Spinal glutamatergic and serotonergic neurotransmission contribute to EMG during skin cooling-evoked shivering2009

    • Author(s)
      Morrison SF, 中村和弘
    • Organizer
      The 3rd International Symposium on Physiology and Pharmacology of Temperature Regulation (PPTR2009)
    • Place of Presentation
      くにびきメッセ (松江市)
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://cp.kyoto-u.ac.jp/Nakamura/nakamura-j.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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