Research for a novel mechanism of prostate cancer progression using a newly established xenograft model

• Project/Area Number: 21890120
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: SHIMIZU Yosuke Kyoto University, 医学研究科, 助教 (00542094)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,509,000 (Direct Cost: ¥1,930,000、Indirect Cost: ¥579,000); Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000); Fiscal Year 2009: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000)
• Keywords: 前立腺癌 / Xenograft / アンドロゲン受容体変異 / ホルモン不応性 / xenograft / androgen receptor mutation

Research Abstract

We established a novel xenograft mouse model mimicking a human prostate cancer. Immunohistochemistry showed AR and PSA protein expression in epithelial cells. Sequencing analysis revealed the AR with a point mutation at amino acid 874 (H874Y). Promoter assay revealed agonistic effect of estradiol against the AR with apoint mutation H874Y.

Research Products

• [Journal Article] SPA-1 controls the invasion and metastasis of human prostate cancer. (2011)
  • Author(s): Shimizu Y.
  • Journal Title: Cancer Science Volume: 102(4) Pages: 828-836
  • NAID: 10029292527
  • Peer Reviewed
• [Journal Article] Activation of Rac1 Is Closely Related to Androgen-Independent Cell Proliferation of Prostate Cancer Cells Both in Vitro and in Vivo. (2010)
  • Author(s): Kobayashi T, Inoue T, Shimizu Y, Ogawa O, Nakamura E.
  • Journal Title: Mol Endocrinol 24 peer review Pages: 722-734
• [Journal Article] SPA-1 controls the invasion and metastasis of human prostate cancer.
  • Author(s): Shimizu Y, Hamazaki Y, Hattori M, Ogawa O, Minato N, Nakamura E.
  • Journal Title: Cancer Sci. in press.peer review
  • NAID: 10029292527
• [Presentation] 嚢胞状腫瘍形成する新規前立腺癌異種移植片モデル (2010)
  • Author(s): 小堀豪
  • Organizer: 第69回 日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] 嚢胞状腫瘍形成する新規前立腺癌異種移植片モデル (2010)
  • Author(s): 小堀豪、井上貴博、岡田能幸、前野淳、寺田直樹、清水洋祐、神波大己、小川修
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] ヒト前立腺癌におけるRap1 Gap SPA-1 を介した転移機序の解明 (2010)
  • Author(s): 清水洋祐
  • Organizer: 第48回日本癌治療学会
  • Place of Presentation: 京都
• [Presentation] 去勢抵抗性前立腺癌の治療標的探索のための新規xenograft におけるmicroRNA 発現解析 (2010)
  • Author(s): 前野淳、岡田能幸、小堀豪、寺田直樹、清水洋祐、井上貴博、神波大己、小川修
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] アンドロゲン受容体変異を持つ新規樹立 xenograft における antiandrogen withdrawal syndrome と alternative antiandrogen therapy (2009)
  • Author(s): 小堀豪
  • Organizer: 第39回アンドロロジーカンファレンス
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-10
• [Remarks] ホームページ等
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/