Study on defense mechanisms of bacteria and host cells in infection.

• Project/Area Number: 21890122
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Osaka University
• Principal Investigator: NISHINO Mitsuko Osaka University, 産業科学研究所, 特任助教(常勤) (30510440)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: 感染症 / 細菌 / オートファジー / 輸送体 / 多剤耐性菌 / 排出蛋白質

Research Abstract

Autophagy is a bulk degradation process in eukaryotic cells and functions as one of defense mechanisms upon bacterial infection. The origin and source of autophagosomal membranes are long-standing questions. We revealed by using both molecular biological tools and electron tomography that the endoplasmic reticulum is a source for autophagosomal membranes.

Research Products

• [Journal Article] Effect of overexpression of small noncoding DsrA RNA on multidrug efflux in Escherichia coli. (2010)
  • Author(s): Nishino K., Yamasaki S., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: J Antimicrob.Chemother 66 Pages: 291-296
  • Peer Reviewed
• [Journal Article] Indoleenhances acid resistance in Escherichia coli. (2010)
  • Author(s): Hirakawa H., Hayashi-Nishino M., Yamaguchi A., Nishino K.
  • Journal Title: Microb.Pathog.(Co-firstauthors) 49 Pages: 90-94
  • Peer Reviewed
• [Journal Article] Effect of NlpE overproduction on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Nishino K., Yamasaki S., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: Antimicrob Agents Chemother. 54 Pages: 2239-2243
  • Peer Reviewed
• [Journal Article] Impact of the RNA chaperone Hfq on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Yamada J., Yamasaki S., Hirakawa H., Hayashi-Nishino M., Yamaguchi A., Nishino K.
  • Journal Title: J.Antimicrob.Chemother. 65 Pages: 853-858
  • Peer Reviewed
• [Journal Article] 電子線トモグラフィーによるオートファジーの解析 (2010)
  • Author(s): 西野-林美都子
  • Journal Title: 顕微鏡 45 Pages: 77-79
• [Journal Article] Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation. (2010)
  • Author(s): Hayashi-Nishino M., Fujita N., Noda T., Yamaguchi A., Yoshimori T., Yamamoto A.
  • Journal Title: Autophagy 6 Pages: 301-303
  • Peer Reviewed
• [Journal Article] Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation. (2010)
  • Author(s): Hayashi-Nishino, M., et al.
  • Journal Title: Autophagy Volume: 6 Pages: 301-303
  • Peer Reviewed
• [Journal Article] Impact of the RNA chaperone Hfq on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Yamada, J., et al
  • Journal Title: Journal of Antimicrobial Chemotherapy Volume: 65 Pages: 853-858
  • Peer Reviewed
• [Journal Article] Effect of NlpE overproduction on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Nishino, K., et al
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 54 Pages: 2239-2243
  • Peer Reviewed
• [Journal Article] Indole enhances acid resistance in Escherichia coli. (2010)
  • Author(s): Hirakawa, H., et al
  • Journal Title: Microbial Pathogenesis Volume: 49 Pages: 90-94
  • Peer Reviewed
• [Journal Article] 電子線トモグラフィーによるオートファジーの解析 (2010)
  • Author(s): 西野-林美都子
  • Journal Title: 顕微鏡 Volume: 45 Pages: 77-79
• [Journal Article] A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation. (2009)
  • Author(s): Hayashi-Nishino M., Fujita N., Noda T., Yamaguchi A., Yoshimori T., Yamamoto A.
  • Journal Title: Nature Cell Biology 11 Pages: 1433-1437
  • Peer Reviewed
• [Journal Article] H-NS modulates multidrug resistance of Salmonella enterica serovar Typhimurium by repressing multidrug efflux genes acrEF. (2009)
  • Author(s): Nishino K., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: Antimicrob.Agents Chemother. 53 Pages: 3541-3543
  • Peer Reviewed
• [Journal Article] Role of the AraC-XylS family regulator YdeO in multi-drug resistance of Escherichia coli. (2009)
  • Author(s): Nishino K., Senda Y., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: J.Antibiot. 62 Pages: 251-257
  • Peer Reviewed
• [Journal Article] A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation. (2009)
  • Author(s): Hayashi-Nishino, M., et al.
  • Journal Title: Nature Cell Biology 11 Pages: 1433-1437
  • Peer Reviewed
• [Journal Article] Role of the AraC-XylS family regulator YdeO in multi-drug resistance of Escherichia coli. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: The Journal of Antibiotics 62 Pages: 251-257
  • Peer Reviewed
• [Journal Article] H-NS modulates multidrug resistance of Salmonella enterica serovar Typhimurium by repressing multidrug efflux genes acrEF. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: Antimicrobial Agents and Chemotherapy 53 Pages: 3541-3543
  • Peer Reviewed
• [Journal Article] Effect of overexpression of small noncoding DsrA RNA on multidrug efflux in Escherichia coli
  • Author(s): Nishino, K., et al
  • Journal Title: Journal of Antimicrobial Chemotherapy Volume: (In press)
  • Peer Reviewed
• [Journal Article] Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation.
  • Author(s): Hayashi-Nishino, M., et al.
  • Journal Title: Autophagy (In press)
  • Peer Reviewed
• [Journal Article] Impact of the RNA chaperone Hfq on multidrug resistance in Escherichia coli.
  • Author(s): Yamada, J., et al.
  • Journal Title: Journal of Antimicrobial Chemotherapy (In press)
  • Peer Reviewed
• [Journal Article] Effect of NlpE overproduction on multidrug resistance in Escherichia coli.
  • Author(s): Nishino, K., et al.
  • Journal Title: Antimicrobial Agents and Chemotherapy (In press)
  • Peer Reviewed
• [Presentation] Development of novel therapeutic strategies to tackle multidrug-resistant pathogens. (2011)
  • Author(s): Nishino, K., et al.
  • Organizer: The American Association for the Advancement of Science (AAAS) 2011 Annual Meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-02-20
• [Presentation] RNA chaperone regulates the multidrug transporter AcrB in Escherichia coli. (2010)
  • Author(s): 山崎聖司, 他
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Role of the RNA Chaperon Hfq in Multidrug Resistance of Escherichia coli (2010)
  • Author(s): Nishino, K., et al.
  • Organizer: NSMS & NZSBMB Joint Meeting
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2010-12-01
• [Presentation] Impact of Hfq on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Yamasaki, S., et al.
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Island
  • Year and Date: 2010-09-09
• [Presentation] 大腸菌多剤耐性化におけるRNAシャペロンHfqの役割 (2010)
  • Author(s): 山崎聖司, 他
  • Organizer: 第22回微生物シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-04
• [Presentation] Overexpressed lipoprotein increases multidrug ressitance by induction of multidrug transporters in E.coli. (2010)
  • Author(s): Yamasaki, S., et al.
  • Organizer: ASM American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-05-25
• [Presentation] Electron Tomography Revealed the Endoplasmic Reticulum as a Source of Autophagosomal Membranes (2010)
  • Author(s): 西野美都子
  • Organizer: ASM American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-05-24
• [Presentation] 大腸菌薬剤排出ポンプ制御におけるNlpEの役割 (2010)
  • Author(s): 山崎聖司, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] Electron Tomography Revealed a Subdomain of the Endoplasmic Reticulum as a Cradle for Autophagosome Formation (2010)
  • Author(s): 西野美都子
  • Organizer: The 13th Sanken International Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2010-01-18
• [Presentation] Electron Tomography Revealed a Subdomain of the Endoplasmic Reticulum as a Cradle for Autophagosome Formation (2010)
  • Author(s): Hayashi-Nishino, M., et al.
  • Organizer: The 13^<th> Sanken International Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2010-01-18
• [Presentation] Electron Tomography Revealed a Subdomain of the Endoplasmic Reticulum as a Cradle for Autophagosome Formation (2009)
  • Author(s): 西野美都子
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2009-12-01
• [Presentation] Electron Tomography Revealed a Subdomain of the Endoplasmic Reticulum as a Cradle for Autophagosome Formation (2009)
  • Author(s): 西野美都子, 他
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-01
• [Presentation] 電子線トモグラフィーにより見いだされた、オートファゴソームを形成する"ゆりかご"としての小胞体サブドメイン、視る生物学4 (2009)
  • Author(s): 西野美都子
  • Organizer: 進化するイメージング
  • Place of Presentation: 奈良先端科学技術大学院大学(奈良県)(招待講演)
  • Year and Date: 2009-11-24
• [Presentation] 電子線トモグラフィーにより見いだされた、オートファゴソームを形成する"ゆりかご"としての小胞体サブドメイン (2009)
  • Author(s): 西野美都子
  • Organizer: 視る生物学4-進化するイメージング-
  • Place of Presentation: 奈良
  • Year and Date: 2009-11-24
• [Presentation] 異物排出トランスポーターによる宿主免疫回避機構の解明 (2009)
  • Author(s): 上田珠美, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Autophagy and the Endoplasmic Reticulum (2009)
  • Author(s): 西野美都子
  • Organizer: 5th International Symposium on Autophagy Meeting
  • Place of Presentation: Otsu, Shiga(招待講演)
  • Year and Date: 2009-09-25
• [Presentation] Autophagy and the Endoplasmic Reticulum (2009)
  • Author(s): Hayashi-Nishino, M., et al.
  • Organizer: 5^<th> International Symposium on Autophagy Meeting
  • Place of Presentation: Otsu, Shiga
  • Year and Date: 2009-09-25
• [Presentation] 電子線トモグラフィーを用いたダイナミン1ノックアウトマウスの神経終末におけるエンドサイトーシス中間体の解析 (2009)
  • Author(s): 西野美都子
  • Organizer: 第52回日本神経化学会大会シンポジウム
  • Place of Presentation: 伊香保温泉ホテル天坊(群馬県)(招待講演)
  • Year and Date: 2009-06-23
• [Presentation] 電子線トモグラフィーを用いたダイナミン1ノックアウトマウスの神経終末におけるエンドサイトーシス中間体の解析 (2009)
  • Author(s): 西野美都子, 他
  • Organizer: 第52回日本神経化学会大会 シンポジウム
  • Place of Presentation: 群馬
  • Year and Date: 2009-06-23
• [Presentation] 電子線トモグラフィーにより見いだされた、オートファゴソームを形成する"ゆりかご"としての小胞体サブドメイン (2009)
  • Author(s): 西野美都子
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-06-04
• [Presentation] 電子線トモグラフィーにより見いだされた、オートファゴソームを形成する"ゆりかご"としての小胞体サブドメイン (2009)
  • Author(s): 西野美都子, 他
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Remarks] 受賞等、特記事項
  • URL: http://www.f1000biology.com/article/id/1331957
• [Remarks] Faculty of 1000 Biology “Must Read"(2009年12)
• [Remarks] 英科学誌Nature Cell Biologyに掲載された論文(論文業績(7))が、-流研究者数十名から構成される論文評価システて、重要な論文として"MustRead"に選出された
• [Remarks] 「日経産業新聞」掲載(2009年11月)
• [Remarks] 包む膜の仕組み解明小胞体が延伸
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/jp/operation/research_activities.html#200911