Development of a novel method of promoting alveolar bone formation by controlling the expression of BMP antagonist.

Research Project

Project/Area Number	21890149
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Prosthetic dentistry
Research Institution	Okayama University
Principal Investigator	FUJISAWA Takuo Okayama University, 岡山大学病院, 助教 (20325096)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	質細胞 / BMP / BMPアンタゴニスト / siRNA / 骨髄由来間質細胞
Research Abstract	The purpose of this study is to identify the BMP antagonists which expressed during osteogenic differentiation induced by BMP-2 and develop a novel method for promoting alveolar bone formation by controlling the expression of those antagonists. In this study, I demonstrate knockdown of SOST by RNA interference in vitro resulted in a significant increase in the expression of the osteogenic marker alkaline phosphatase and the deposition of extracellular mineral, in response to osteogenic stimulation.