| Budget Amount *help |
¥2,587,000 (Direct Cost: ¥1,990,000、Indirect Cost: ¥597,000)
Fiscal Year 2010: ¥1,222,000 (Direct Cost: ¥940,000、Indirect Cost: ¥282,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Research Abstract |
Dentin phosphoprotein (DPP) and dentin sialoprotein (DSP) are cleaved products of precursor dentin sialophosphoprotein (DSPP). In this research, we explored the mechanisms and the biological significance of DSPP cleavage into two proteins using cell lines expressing cleavage-resistant DSPP. We observed DSP specific band under 100kDa from the condition medium of normal DSPP transfectants. Conversely, we detected DSP and DPP specific bands over 100kDa from mutated DSPP transfectants. Interestingly, the intensity of DSP and DPP specific bands of mutated DSPP was apparently higher than that of normal DSPP. These results suggested that we successfully generated stable trasnfectants expressing normal or mutated DSPP and cleavage of DSPP might affect the secretion or the extracellular deposition of DSP and DPP.
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