Applications of amelogenin to the induction of bone regeneration by using mesenchymal stem cells

• Project/Area Number: 21890164
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: TANNE Yuki Hiroshima University, 医歯薬学総合研究科, 助教 (50526241)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,496,000 (Direct Cost: ¥1,920,000、Indirect Cost: ¥576,000); Fiscal Year 2010: ¥1,144,000 (Direct Cost: ¥880,000、Indirect Cost: ¥264,000); Fiscal Year 2009: ¥1,352,000 (Direct Cost: ¥1,040,000、Indirect Cost: ¥312,000)
• Keywords: アメロゲニン / 未分化間葉系幹細胞 / 骨再生治療 / エナメル蛋白

Research Abstract

It is demonstrated that amelogenin increases the proliferation of MSCs by an interaction with LAMP1 through MAPK-ERK signaling pathway. In addition, amelogenin up-regulated the expressions of bone specific marker and mineralization activity in the osteogenic differentiation of MSCs. Amelogenin increases the expression of BMP-6, known as a promoting factor for bone differentiation.

Research Products

• [Journal Article] Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow (2010)
  • Author(s): Huang YC, Tanimoto K, Tanne Y, et al.
  • Journal Title: Cell Tissue Res 342 Pages: 205-212
  • Peer Reviewed
• [Journal Article] Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow (2010)
  • Author(s): Huang YC
  • Journal Title: Cell Tissue Res Volume: 342 Pages: 205-212
  • Peer Reviewed
• [Presentation] Amelogenin Modulates the Proliferation of Human Mesenchymal Stem Cells through the MAPK-ERK Pathway (2010)
  • Author(s): Huang Yu-ching
  • Organizer: 88th IADR General Session
  • Place of Presentation: Spain
  • Year and Date: 2010-07-16
• [Presentation] 歯周組織構成細胞に対するヒトリコンビナントアメロゲニンの効果とシグナル伝達機構の解明 (2010)
  • Author(s): 国松亮
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
• [Presentation] Amelogenin Modulates the Proliferation of Human Mesenchymal Stem Cells through the MAPK-ERK Pathway (2010)
  • Author(s): Huang Yu-ching
  • Organizer: 88th IADR General Session
  • Place of Presentation: Barcelona, Spain
• [Presentation] Regeneration of maxillary bone with mesenchymal stem cells for the treatment of jaw cleft (2009)
  • Author(s): Yoshioka Motoko
  • Organizer: 3rd Hiroshima Conference on education and Science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 2009-11-07
• [Presentation] Regeneration of maxillary bone with mesenchymal stem cells for the treatment of jaw cleft (2009)
  • Author(s): Motoko Yoshioka
  • Organizer: 3rd Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 2009-11-07
• [Presentation] Bone regeneration for the treatment of jaw cleft with mesenchymal stem cells derived from bone marrow (2009)
  • Author(s): Motoko Yoshioka
  • Organizer: 11^<th> International Congress, CLEFT 2009
  • Place of Presentation: Brazil Fortaleza
  • Year and Date: 2009-09-10
• [Presentation] Effects of Amelogenin on the Proliferation and Differentiation of Human Mesenchymal Stem Cells (2009)
  • Author(s): Huang Yu-Ching
  • Organizer: Workshop on Bio-Dental Education and Research in Hiroshima
  • Place of Presentation: Hiroshima