| Budget Amount *help |
¥2,184,000 (Direct Cost: ¥1,680,000、Indirect Cost: ¥504,000)
Fiscal Year 2010: ¥793,000 (Direct Cost: ¥610,000、Indirect Cost: ¥183,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Research Abstract |
For the examination of novel epitopes that can effectively inhibit HIV infection, we immunized soluble CD4 and membrane fusion intermediate complex of HIV envelope-CD4-chemikine receptor that is formed on the cell membrane of CD4+ T cell during the infection of HIV, to the high-affinity antibody (Ab) producing mice. And we established anti-CD4 monoclonal (m) Ab and anti-fusion complex (FC) mAb libraries. Anti-CD4 R275 mAb recognized novel epitope of domain three of CD4 and inhibited HIV-1 infection. We established anti-FC mAb library that recognized dynamic epitopes appearing during the membrane fusion process. Our mAb library may be useful on the examination of novel target of inhibition of HIV infection.
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