The development of novel cancer-immunotherapy utilizing dendritic cells derived from iPS cells.

• Project/Area Number: 21890199
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Kumamoto University
• Principal Investigator: FUKUSHIMA Satoshi Kumamoto University, 医学部附属病院, 助教 (50398210)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000); Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
• Keywords: iPS細胞 / 樹状細胞 / 癌免疫療法 / 多能性幹細胞 / 免疫療法 / がん抗原 / IL-15 / 悪性黒色腫 / 腫瘍マーカー / メラノーマ / 腫瘍免疫

Research Abstract

To develop the novel cell-vaccine against the advanced malignant melanoma, we conducted the experiments using the genetically modified iPS cell derived dendritic cells. We found that the dendritic cells could be generated from any clones of mouse iPS cells. We found that KIF20A is highly expressed in malignant melanoma. In addition, we found that micro RNA-221 could be a new tumor marker of malignant melanoama.

Research Products

• [Journal Article] The circulating microRNA-221 level in patients with malignant melanoma as a new tumor marker. (2011)
  • Author(s): Kanemaru H, Fukushima S, Yamashita J, Honda N, Oyama R, Kakimoto A, Masuguchi S, Ishihara T, Inoue Y, Jinnin M, Ihn H.
  • Journal Title: J Dermatol Sci. 61巻 Pages: 187-193
  • NAID: 10030890452
  • Peer Reviewed
• [Journal Article] Circulating microRNA associated with TNF-α signaling pathway in patients with plaque psoriasis. (2011)
  • Author(s): Oyama R, Jinnin M, Kakimoto A, Kanemaru H, Ichihara A, Fujisawa A, Honda N, Masuguchi S, Fukushima S, Maruo K, Ihn H.
  • Journal Title: J Dermatol Sci. 61巻 Pages: 209-211
  • NAID: 10030890580
  • Peer Reviewed
• [Journal Article] Circulating miR-29a levels in patients with scleroderma spectrum disorder. (2011)
  • Author(s): Kawashita Y, Jinnin M, Makino T, Kajihara I, Makino K, Honda N, Masuguchi S, Fukushima S, Inoue Y, Ihn H.
  • Journal Title: J Dermatol Sci. 61巻 Pages: 67-69
  • NAID: 10030889851
  • Peer Reviewed
• [Journal Article] Expression of matrix metalloproteinase-13 is controlled by IL-13 via PI3K/Akt3 and PKC-δ in normal human dermal fibroblasts. (2011)
  • Author(s): Moriya C, Jinnin M, Yamane K, Maruo K, Muchemwa FC, Igata T, Makino T, Fukushima S, Ihn H.
  • Journal Title: J Invest Dermatol. 131巻 Pages: 655-661
  • Peer Reviewed
• [Journal Article] Cutaneous hyalohyphomycosis by Scedosporium apiospermum in an immunocompromised patient. (2011)
  • Author(s): Makino K, Fukushima S, Maruo K, Egawa K, Nishimoto K, Ihn H.
  • Journal Title: Mycoses. 54巻 Pages: 259-261
  • Peer Reviewed
• [Journal Article] The circulating microRNA-221 level in patients with malignant melanoma as a new tumor marker. (2011)
  • Author(s): Kanemaru H
  • Journal Title: J Dermatol Sci Volume: 61 Pages: 187-193
  • Peer Reviewed
• [Journal Article] Circulating microRNA associated with TNF-α signaling pathway inpatients with plaque psoriasis. (2011)
  • Author(s): Oyama R
  • Journal Title: J Dermatol Sci Volume: 61 Pages: 209-211
  • Peer Reviewed
• [Journal Article] Expression of matrix metalloproteinase-13 is controlled by IL-13 via PI3K/Akt3 and PKC-δ in normal human dermal fibroblasts. (2011)
  • Author(s): Moriya C
  • Journal Title: J Invest Dermatol Volume: 131 Pages: 655-611
  • Peer Reviewed
• [Journal Article] Circulating miR-29a levels in patients with scleroderma spectrum disorder. (2011)
  • Author(s): Kawashita Y
  • Journal Title: J Dermatol Sci Volume: 61 Pages: 67-69
  • Peer Reviewed
• [Journal Article] がん抗原とα-GalCerを負荷した樹状細胞による腫瘍免疫の増強 (2011)
  • Author(s): 福島聡
  • Journal Title: 臨床免疫・アレルギー科 Volume: 55 Pages: 262-268
• [Journal Article] Related citations Pluripotent stem cell-derived dendritic cells for immunotherapy. (2010)
  • Author(s): Senju S, Matsunaga Y, Fukushima S, Hirata S, Matsuyoshi H, Nishimura Y.
  • Journal Title: Front Biosci. 2巻 Pages: 1520-1527
• [Journal Article] Transglutaminasel Preferred Substrate Peptide K5 Is an Efficient Tool in Diagnosis of Lamellar Ichthyosis. (2010)
  • Author(s): Akiyama M, Sakai K, Yanagi T, Fukushima S, Ihn H, Hitomi K, Shimizu H.
  • Journal Title: Am J Pathol. 176巻 Pages: 1592-1599
  • Peer Reviewed
• [Journal Article] Pluripotent stem cells as source of dendritic cells for immune therapy. (2010)
  • Author(s): Senju S, Hirata S, Motomura Y, Fukuma D, Matsunaga Y, Fukushima S, Matsuyoshi H, Nishimura Y.
  • Journal Title: Int J Hematol. 91巻 Pages: 392-400
  • NAID: 10026401004
• [Journal Article] Down-regulation of mir-424 contributes to the abnormal angiogenesis via MEK1 and cyclin E1 in senile hemangioma : its implications to therapy. (2010)
  • Author(s): Nakashima T, Jinnin M, Etoh T, Fukushima S, Masuguchi S, Maruo K, Inoue Y, Ishihara T, Ihn H.
  • Journal Title: PLoS One. 5巻
  • Peer Reviewed
• [Journal Article] Dual effects of TRAIL in suppression of autoimmunity : the inhibition of Th1 cells and the promotion of regulatory T cells. (2010)
  • Author(s): Ikeda T, Hirata S, Fukushima S, Matsunaga Y, Ito T, Uchino M, Nishimura Y, Senju S.
  • Journal Title: J Immunol. 185巻 Pages: 5259-5267
  • Peer Reviewed
• [Journal Article] Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis. (2010)
  • Author(s): Higashi-Kuwata N, Jinnin M, Makino T, Fukushima S, Inoue Y, Muchemwa FC, Yonemura Y, Komohara Y, Takeya M, Mitsuya H, Ihn H.
  • Journal Title: Arthritis Res Ther. 12巻
  • Peer Reviewed
• [Journal Article] Down-regulation of mir-424 contributes to the abnormal angiogenesis via MEK1 and cyclin E1 in senile hemangioma : its implications to therapy (2010)
  • Author(s): Nakashima T
  • Journal Title: PLoS One Volume: 14
  • Peer Reviewed
• [Journal Article] Serum levels of soluble CD163 in patients with systemic sclerosis. (2010)
  • Author(s): Nakayama W
  • Journal Title: Rheumatol Int.
  • Peer Reviewed
• [Journal Article] Dual effects of TRAIL in suppression of autoimmunity : the inhibition of Th1 cells and the promotion of regulatory T cells. (2010)
  • Author(s): Ikeda T
  • Journal Title: J Immunol. Volume: 185 Pages: 5259-5267
  • Peer Reviewed
• [Journal Article] Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis (2010)
  • Author(s): Higashi-Kuwata N
  • Journal Title: Arthritis Res Ther Volume: 12
  • Peer Reviewed
• [Journal Article] Pluripotent stem cell-derived dendritic cells for immunotherapy. (2010)
  • Author(s): Senju S
  • Journal Title: Front Biosci Volume: 2 Pages: 1520-1527
  • Peer Reviewed
• [Journal Article] Transglutaminase1 preferred substrate peptide K5 is an efficient tool in diagnosis of lamellar ichthyosis. (2010)
  • Author(s): Akiyama M
  • Journal Title: Am J Pathol Volume: 176 Pages: 1592-1599
  • Peer Reviewed
• [Journal Article] Pluripotent stem cells as source of dendritic cells for immune therapy. (2010)
  • Author(s): Senju S
  • Journal Title: Int J Hematol. Volume: 91 Pages: 392-400
• [Journal Article] Pluripotent stem cells as source of dendritic cells for immune therapy (2010)
  • Author(s): Senju S
  • Journal Title: International Journal of Hematology 91 Pages: 392-400
  • Peer Reviewed
• [Journal Article] ES細胞由来樹状細胞を用いたメラノーマの免疫療法 (2009)
  • Author(s): 福島聡
  • Journal Title: 医薬の門 49巻 Pages: 355-359
• [Journal Article] ES細胞 (2009)
  • Author(s): 福島聡, 西村泰治, 千住覚
  • Journal Title: iPS細胞由来の樹状細胞を利用したワクチン臨床免疫・アレルギー科 52巻 Pages: 331-338
• [Journal Article] ES細胞由来樹状細胞を用いたメラノーマの免疫療法 (2009)
  • Author(s): 福島聡
  • Journal Title: 医薬の門 49 Pages: 89-93
• [Journal Article] ES細胞、iPS細胞由来の樹状細胞を利用したワクチン (2009)
  • Author(s): 福島聡
  • Journal Title: 臨床免疫・アレルギー科 52 Pages: 331-338
  • NAID: 40016843476
• [Journal Article] Serum levels of soluble CD163 in patients with systemic sclerosis.
  • Author(s): Nakayama W, Jinnin M, Makino K, Kajihara I, Makino T, Fukushima S, Inoue Y, Ihn H.
  • Journal Title: Rheumatol Int. 2010 [Epub ahead of print]
  • Peer Reviewed
• [Presentation] 口蓋悪性黒色腫の一例 (2011)
  • Author(s): 福島聡
  • Organizer: 第53回日本皮膚科学会愛媛地方会
  • Place of Presentation: 全日空ホテル、松山
  • Year and Date: 2011-03-19
• [Presentation] 多能性幹細胞由来樹状細胞を用いたワクチン療法 (2011)
  • Author(s): 福島聡
  • Organizer: 第8回日本免疫治療学研究会核術集会
  • Place of Presentation: 東京ガーデンパレス、東京
  • Year and Date: 2011-02-26
• [Presentation] 悪性黒色腫肺転移に対するラジオ波焼灼療法 (2011)
  • Author(s): 福島聡
  • Organizer: 第134回日本皮膚科学会徳島地方会
  • Place of Presentation: ホテルグランドパレス、徳島
  • Year and Date: 2011-01-29
• [Presentation] 2000-2010年の中面班メラノーマグループにおけるstage IV患者のまとめ (2011)
  • Author(s): 福島聡
  • Organizer: H22厚生労働科学研究費補助金・医療技術実用化・中面班 平成22年度第2回メラノーマグループ班会議
  • Place of Presentation: 国立がん研究センター東病院、柏
  • Year and Date: 2011-01-08
• [Presentation] 新規癌関連抗原KIF20Aのメラノーマにおける発現解析 (2010)
  • Author(s): 福島聡
  • Organizer: 平成22年度厚生労働省がん助成金「悪性黒色腫に対する新しい診療体系の確立に関する研究」第2回班会議
  • Place of Presentation: 国立がん研究センター中央病院
  • Year and Date: 2010-10-16
• [Presentation] Increased serum levels of secreted protein acidic and rich in cysteine in patients with localized scleroderma (2010)
  • Author(s): Fukushima S
  • Organizer: 40^<th> Annual ESDR meeting
  • Place of Presentation: Scandic Marina Congress Center, Helsinki
  • Year and Date: 2010-09-11
• [Presentation] Immunotherapy with genetically modified dendritic cells derived from stem cells against mouse melanoma (2010)
  • Author(s): 福島聡
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-25
• [Presentation] Immunotherapy with genetically modified dendritic cells derived from stem cells against mouse melanoma (2010)
  • Author(s): Fukushima S
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe Convention Center, Kobe
  • Year and Date: 2010-08-25
• [Presentation] 肺転移巣に対するラジオ波焼灼療法(RFA) (2010)
  • Author(s): 福島聡
  • Organizer: 平成22年度厚生労働省がん助成金「悪性黒色腫に対する新しい診療体系の確立に関する研究」第1回班会議
  • Place of Presentation: 国立がん研究センター中央病院
  • Year and Date: 2010-07-31
• [Presentation] 悪性黒色腫肺転移に対するラジオ波焼灼療法(REA) (2010)
  • Author(s): 福島聡
  • Organizer: 平成22年度 がん研究開発費(山崎班)第一回班会議
  • Place of Presentation: 国立がん研究センター中央病院、東京
  • Year and Date: 2010-07-31
• [Presentation] メラノーマにおける新規癌関連抗原RAB6KIFLの発現解析 (2010)
  • Author(s): 山下淳二
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: KKRホテル熊本、熊本
  • Year and Date: 2010-07-22
• [Presentation] 切除不能のStage IV全体とそのうちのダカルバジン投与群の病勢制御率・奏功率・全生存期間・無増悪期間のhistorical controlのデータ (2010)
  • Author(s): 福島聡
  • Organizer: H22厚生労働科学研究費補助金・医療技術実用化・中・面班 平成22年度第1回メラノーマグループ班会議
  • Place of Presentation: 国立がん研究センター東病院、柏
  • Year and Date: 2010-07-03
• [Presentation] 口蓋悪性黒色腫の1例 (2010)
  • Author(s): 福島聡
  • Organizer: 第26回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2010-06-04
• [Presentation] 多能性幹細胞由来樹状細胞と免疫療法 (2010)
  • Author(s): 福島聡
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 2010-04-17
• [Presentation] Analysis of serum secreted protein acidic and rich in cysteine levels in patients with localized scleroderma. (2010)
  • Author(s): 福島聡
  • Organizer: The 35th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 和歌山県立文化ホール
• [Presentation] Increased serum levels of secreted protein acidic and rich in cysteine in patients with localized scleroderma. (2010)
  • Author(s): 福島聡
  • Organizer: 40th Annual ESDR Meeting
  • Place of Presentation: Marina Congress Center・Finland
• [Presentation] メラノーマにおける新規癌関連抗原RAB6KIFLの発現解析 (2010)
  • Author(s): 山下淳二、福島聡, 他
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: KKRホテル熊本
• [Presentation] 口蓋悪性黒色腫の1例 (2010)
  • Author(s): 福島聡
  • Organizer: 第26回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 京王プラザ
• [Presentation] 遺伝子改変iPS細胞由来樹状細胞およびマクロファージによるメラノーマの免疫療法 (2010)
  • Author(s): 福島聡
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 多能性幹細胞由来樹状細胞を用いた免疫療法 (2009)
  • Author(s): 福島聡
  • Organizer: 第73回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 甲府富士屋ホテル
• [Presentation] Immunothrapy with Multiple Antigen-Targeted and Genetically Modified Dendritic Cells Derived from Pluripotent Stem Cells Against Mouse Melanoma (2009)
  • Author(s): 福島聡
  • Organizer: 7th World Congress on melanoma 5th Congress of European Association of Dermato-Oncology (EADO)
  • Place of Presentation: Hofburg Viena・Austria
• [Presentation] Immunothrapy with Multiple Antigen-Targeted and Genetically Modified Dendritic Cells Derived from Pluripotent Stem Cells Against Mouse Melanoma (2009)
  • Author(s): 福島聡
  • Organizer: 7^<th> World Congress on melanoma and 5^<th> Congress of European Association of Dermato-Oncology(EADO)
  • Place of Presentation: Hofburg, Vienna, Austria
• [Presentation] 多能性幹細胞由来樹状細胞を用いた免疫療法 (2009)
  • Author(s): 福島聡
  • Organizer: 第73回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 甲府富士屋ホテル、甲府市