| Budget Amount *help |
¥3,341,000 (Direct Cost: ¥2,570,000、Indirect Cost: ¥771,000)
Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
Fiscal Year 2009: ¥1,885,000 (Direct Cost: ¥1,450,000、Indirect Cost: ¥435,000)
|
|---|
| Research Abstract |
In this study, our objective is to identify novel p53-target genes to regulate autophagy using microarray. First, we established the p53 knock-down and the control cell lines, and Each cell lines were collected after amino acid starvation. Total cellular RNA was extracted and purified. We purified cRNA from total RNA, and performed cDNA microarray. Now, we are evaluating the difference in gene expressions by the presense of p53 during induction of autophagy.
|
