Function of p53 tumor-suppressor gene mediated autophagy
| Project/Area Number |
21890200
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
MIYAMOTO yuji Kumamoto University, 医学部附属病院, 助教 (80551259)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,341,000 (Direct Cost: ¥2,570,000、Indirect Cost: ¥771,000)
Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
Fiscal Year 2009: ¥1,885,000 (Direct Cost: ¥1,450,000、Indirect Cost: ¥435,000)
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| Keywords | オートファジー / p53 / 発癌 / p53遺伝子 / cDNA |
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| Research Abstract |
In this study, our objective is to identify novel p53-target genes to regulate autophagy using microarray. First, we established the p53 knock-down and the control cell lines, and Each cell lines were collected after amino acid starvation. Total cellular RNA was extracted and purified. We purified cRNA from total RNA, and performed cDNA microarray. Now, we are evaluating the difference in gene expressions by the presense of p53 during induction of autophagy.
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Report
(3 results)
Research Products
(12 results)
