Elucidation of carbohydrate chains recognition mechanism and application to medicinal chemistry

• Project/Area Number: 21890258
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Chemical pharmacy
• Research Institution: Teikyo University
• Principal Investigator: NISHIYAMA Kazusa Teikyo University, 薬学部, 助教 (40545612)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: 有機化学 / 糖鎖合成 / 分子認識 / レクチン / エーテル結合糖 / QCM / ファージ / ディスプレイ / 分子プローブ / ファージデイスプレイ / ファージディスプレイ

Research Abstract

The saccharide acts as information element in the life phenomenon having a complicated conformation. The establishment of new analytical method is necessary to elucidate the recognition mechanism of such carbohydrate chains. I established two kinds of new carbohydrate chain analytical methods in this study by putting organic synthetic chemistry and biochemistry and molecular biology together. One is the phage display method using the self-assembled monolayer and the other is the fluorescence label method which maintained structure of the carbohydrate chains.

Research Products

• [Journal Article] Synthesis of Fluorescence-Labeled Galβ1-3Fuc and Galβ1-4Fuc as Probes for the Endogenous Glyco-Epitope Recognized by Galectins (2010)
  • Author(s): Kazusa Nishiyama, Atsushi Yamada, Miki Takahashi, Tomoharu Takeuchi, Ken-ichi Kasai, Susumu Kobayashi, Hideaki Natsugari, Hideyo Takahashi
  • Journal Title: Caenorhabditis elegans, Chemical&pharmaceutical Bulletin 58(4) Pages: 495-500
  • Peer Reviewed
• [Journal Article] Novel glycosidation of α-D-altropyranosides (2010)
  • Author(s): Takahiro Nakayama, Kazusa Nishiyama, Hideaki Natsugari, Hideyo Takahashi
  • Journal Title: Synthesis 1 Pages: 43-48
  • Peer Reviewed
• [Journal Article] Synthesis of Fluorescence-Labeled Galβ1-3Fuc and Galβ1-4Fuc as Probes for the Endogenous Glyco-Epitope Recognized by Galectins in Caenorhabditis elegans (2010)
  • Author(s): Kazusa Nishiyama, et al
  • Journal Title: Chem.Pharm.Bull. Volume: 58 Pages: 49-50
  • Peer Reviewed
• [Journal Article] Novel glycosidation of α-D-altropyranosides (2010)
  • Author(s): Takahiro Nakayama
  • Journal Title: Synthesis 1 Pages: 43-48
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans galectins LEC-6 and LEC-1 recognize a chemically synthesized Galβ 1-4Fuc disaccharide unit which is present in Protostomia glycoconjugates (2009)
  • Author(s): Tomoharu Takeuchi, Kazusa Nishiyama, Ken-ichi Sugiura, Miki Takahashi, Atsushi Yamada, Susumu Kobayashi, Hideyo Takahashi, Hideaki Natsugari, Ken-ichi Kasai
  • Journal Title: Glycobiology 19(12) Pages: 1503-1510
  • NAID: 120005518447
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans galectins LEC-6 and LEC-1 recognize a chemically synthesized Ga1β1-4Fuc disaccharide unit which is presentin Protostomia glycoconjugates (2009)
  • Author(s): Tomoharu Takeuchi
  • Journal Title: Glycobiology 19 Pages: 1503-1510
  • Peer Reviewed
• [Presentation] 線虫内在性糖鎖の分子プローブ化及び標的タンパク質の探索 (2011)
  • Author(s): 西山和沙,他
  • Organizer: 第131回日本薬学会年会
  • Place of Presentation: ツインメッセ静岡
• [Presentation] 線虫ガレクチンLEC-6による糖鎖認識機構解明のための分子プローブの合成及びその評価 (2010)
  • Author(s): 西山和沙,他
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: グランシップ(静岡県コンベンションアーツセンター)
• [Presentation] 線虫ガレクチンLEC-6結合糖鎖の認識機構解明のための分子プローブの合成 (2010)
  • Author(s): 西山和沙,他
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山大学
• [Presentation] 線虫ガレクチンLEC-6による糖鎖認識機構解明のための分子プローブの合成及びその評価 (2010)
  • Author(s): 西山和沙, 他
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: 静岡県コンベンションアーツセンター
• [Presentation] Galβ(1→4)Fuc固定化アフィニティカラムの作成及びガレクチン結合能の検討 (2009)
  • Author(s): 山田篤
  • Organizer: 第53回日本薬学会関東支部大会
  • Place of Presentation: 城西大学
  • Year and Date: 2009-10-03