| Project/Area Number |
21890267
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
XIAO Li 日本歯科大学, 生命歯学部, 講師 (80548256)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,793,710 (Direct Cost: ¥2,149,008、Indirect Cost: ¥644,702)
Fiscal Year 2011: ¥245,710 (Direct Cost: ¥189,008、Indirect Cost: ¥56,702)
Fiscal Year 2010: ¥1,196,000 (Direct Cost: ¥920,000、Indirect Cost: ¥276,000)
Fiscal Year 2009: ¥1,352,000 (Direct Cost: ¥1,040,000、Indirect Cost: ¥312,000)
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| Keywords | 再生歯モデル / 上皮陥入 / 上皮間葉相互作用 / 再生促進漢方薬剤 / 新規細胞生存力、石灰化測定法 |
|---|
| Research Abstract |
Epithelium invagination is the key feature of early tooth development. In this study, we first built a three dimensional model to represent epithelium invagination-like structure by tissue engineering using human normal oral epithelial cells(OECs) and dental pulp stem cells(DPSCs). OEC-DPSC co-cultures on matrigel showed typical invagination of epithelial cells and condensation of the underlying mesenchymal cells in a three-dimensional manner. Epithelial invagination related molecules, CD44 and E-cadherin, and mesenchymal condensation involved molecules, N-cadherin and Msx1 expressed at a high level in the tissue model, suggesting the epithelial invagination is functional. Our results suggest that co-cultivated OECs and DPSCs on matrigel under certain conditions can build an epithelium invagination-like model. This model might be explored as a potential research tool for epithelial-mesenchymal interaction and tooth regeneration.
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