| Project/Area Number |
21890277
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
MIZUTANI Yasuyoshi Fujita Health University, 医学部, 助教 (10546229)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,548,000 (Direct Cost: ¥1,960,000、Indirect Cost: ¥588,000)
Fiscal Year 2010: ¥1,183,000 (Direct Cost: ¥910,000、Indirect Cost: ¥273,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Keywords | 酵素抗原法 / 抗体産生細胞 / 無細胞タンパク質合成系 / 関節リウマチ / 自己免疫疾患 / 自己抗原 / AlphaScreen / 免疫組織化学 / コムギ無細胞タンパク合成 / Alpha Screen |
|---|
| Research Abstract |
To identify antigens against antibodies produced within rheumatoid arthritis (RA) synovitis, antigens recognized by antibodies in sera and synovial tissue extracts were screened with the AlphaScreen method. For screening, human protein library was constructed by the wheat germ cell-free protein synthesis system. As a result, a number of proteins were detected. A part of antigens showing high signals in synovitis tissue were applied to the enzyme-labeled antigen method. Specific antibodies against two antigens were visualized in plasma cells in RA synovitis. The present study indicated the usefulness of screening of the protein library with the AlphaScreen method for detecting antibodies in lesional tissue.
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