Carcinogenesis caused by tobacco and alcohol for human tongue keratinocytes (HTK) cell line

Research Project

Project/Area Number	21890287
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Surgical dentistry
Research Institution	Hyogo College of Medicine
Principal Investigator	SEGAWA Emi Hyogo College of Medicine, 医学部, 助教 (00553778)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,431,000 (Direct Cost: ¥1,870,000、Indirect Cost: ¥561,000) Fiscal Year 2010: ¥1,157,000 (Direct Cost: ¥890,000、Indirect Cost: ¥267,000) Fiscal Year 2009: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000)
Keywords	口腔癌 / HPV / 発癌
Research Abstract	To elucidate the carcinogenesis process, we newly established human tongue keratinocytes (HTK) cell lines derived from tongue mucosa immortalized by infection with retroviral vectors expression HPV16 E6/E7. Furthermore, treatment of HPV16 E6/E7 with NNK and EtOH in vitro, and HTK1-16E6E7-NNK + EtOH was established. Then we examined a change of the cell biology in both cells and identified the responsibility gene in the carcinogenesis in oral squamous cell carcinoma.