Elucidation of membrane dynamics and molecular mechanism of bone resorption in osteoclast by using multi-photon microscopy

Research Project

Project/Area Number	21890303
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Physical pharmacy
Research Institution	Hokkaido University
Principal Investigator	HIBI Terumasa Hokkaido University, 電子科学研究所, 助教 (50554292)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000) Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000) Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
Keywords	骨代謝 / 小胞輸送 / バイオイメージング / 多光子顕微鏡 / 蛍光蛋白質
Research Abstract	Bone is maintained by a balance between bone formation and resorption. The osteoclast is the cell that plays a major role in bone resorption in our body. In this study, to elucidate the molecular mechanism of bone resorption, vesicular trafficking in osteoclast was analyzed. By carrying out 4D-imaging, it becomes possible to analyze dynamics of acidic vesicles, probably involved in bone resorption, in living osteoclast not only horizontally but also vertically.