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The study of diagnosis for Parkinson disease at early-stage using with monoclonal antibodies against oxidized DJ-1.

Research Project

Project/Area Number 21890315
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Public health/Health science
Research InstitutionMukogawa Women's University (2010)
National Institute of Advanced Industrial Science and Technology (2009)

Principal Investigator

AKAZAWA Yoko  Mukogawa Women's University, 薬学部, 助手 (50549897)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,457,000 (Direct Cost: ¥1,890,000、Indirect Cost: ¥567,000)
Fiscal Year 2010: ¥1,157,000 (Direct Cost: ¥890,000、Indirect Cost: ¥267,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords蛋白質 / 脳神経疾患 / プロテオーム / 老化 / パーキンソン病 / アルツハイマー病 / 酸化修飾DJ-1 / 簡易診断
Research Abstract

The molecular mechanisms leading to neurodegeneration in Parkinson disease (PD) remain elusive, although many studies of evidence have indicated that oxidative stress and impairment of protein degradation system, resulting in cell death. However, idiopathic PD is essentially diagnosed clinically. The development of biomarkers for PD would afford many advantages : early diagnosis and identification of subgroups of PD. DJ-1 had recently found to be causative gene for a familial form of PD. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 and a competitive enzyme-linked immunosorbent assay (ELISA) for detecting oxidized DJ-1, and measured blood levels of oxidized DJ-1 in PD patients. It was observed that the level and an aggregated form of oxidized DJ-1 in erythrocytes of unmedicated PD patients were markedly higher than mediacted PD patients and healthy subjects. Furthermore, we observed that the treatment of mice with PD model compounds results in a significant elevation of oxidized DJ-1 in erythrocytes and its positive cells in the substantia nigra in a dose-and time-dependent manner. These results suggest the involvement of oxidative modification of DJ-1 in the pathogenesis of PD.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals2010

    • Author(s)
      赤澤陽子、斎藤芳郎、浜窪隆雄, 他
    • Journal Title

      Neuroscience Letters 483

      Pages: 201-205

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals.2010

    • Author(s)
      赤澤陽子、斎藤芳郎, 他
    • Journal Title

      Neuroscience Letters

      Volume: VOL.483 Pages: 201-205

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] パーキンソン病モデルにおける脳内および赤血球酸化DJ-1の増加2010

    • Author(s)
      斎藤芳郎、赤澤陽子, 他
    • Organizer
      第63回日本酸化ストレス学会学術集会
    • Place of Presentation
      神奈川県民ホール
    • Year and Date
      2010-06-24
    • Related Report
      2010 Final Research Report
  • [Presentation] パーキンソン病モデル動物における脳内および赤血球酸化DJ-1の増加2010

    • Author(s)
      斎藤芳郎、赤澤陽子, 他
    • Organizer
      第63回日本酸化ストレス学会学術集会
    • Place of Presentation
      神奈川県民ホール
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酸化DJ-1とパーキンソン病(2):臨床検体の測定2009

    • Author(s)
      赤澤陽子、斎藤芳郎, 他
    • Organizer
      第62回日本酸化ストレス学会学術集会
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2009-06-11
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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