| Project/Area Number |
21890317
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
HARADA Tetsuya Osaka Prefectural Institute of Public Health, 感染症部, 研究員 (70516723)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,275,000 (Direct Cost: ¥1,750,000、Indirect Cost: ¥525,000)
Fiscal Year 2010: ¥975,000 (Direct Cost: ¥750,000、Indirect Cost: ¥225,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 公衆衛生 / 分子疫学解析法 / サルモネラ |
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| Research Abstract |
We aimed to develop a new molecular analysis method for differentiating Salmonella enterica serovar Montevideo, Salmonella Infantis and Salmonella Schwarzengrund isolates in Osaka Prefecture, Japan. We described a multiple-locus variable-number tandem repeats (VNTR) analysis (MLVA) method based on agarose gel electrophoresis separation of polymerase chain reaction products of three simple VNTR loci in Salmonella Montevideo. We believe that the MLVA method can facilitate assessment of the molecular epidemiology of Salmonella Montevideo isolates in light of its present utility in laboratories without a PFGE apparatus or capillary array electrophoresis devices.
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