Search for Proteomics-based analysis of non-alcoholic fatty liver diseases (NAFLD/NASH) in mice.

• Project/Area Number: 21890318
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: National Institute of Biomedical Innovation
• Principal Investigator: NAGANO Kazuya National Institute of Biomedical Innovation, 創薬基盤研究部バイオ創薬プロジェクト, 研究員 (40548301)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: メタボリックシンドローム / Non-alcoholic steatohepatitis(NASH) / Non-alcoholic fatty liver disease(NAFLD) / バイオマーカー / NAFLD / NASH / 抗体プロテオミクス技術

Research Abstract

It has been estimated that non-alcoholic steatohepatitis (NASH) and non-alcoholic fatty liver disease (NAFLD), early stage of NASH, become main factors to cause liver cancer and hepatic cirrhosis in future. Therefore, development of diagnostic and therapeutic techniques is urgently needed. However these diseases are hardly known, due to having been recently recognized. In this study, we searched for NAFLD-related proteins and resulted in successfully identifying 8 kinds of candidate proteins more highly expressed in liver of NAFALD model mice.

Research Products

• [Journal Article] Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung. (2011)
  • Author(s): Yoshida Y., Yamashita T., Nagano K., Imai S., Nabeshi H., Yoshikawa T., Yoshioka Y., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
  • Journal Title: Biochem.Biophys.Res.Commun. 405(4) Pages: 610-614
  • Peer Reviewed
• [Journal Article] Development of a novel antibody proteomics system using a phage antibody library for efficient screening of tumor-related biomarker proteins. (2011)
  • Author(s): Imai S., Nagano K., Yoshida Y., Okamura T., Yamashita T., Abe Y., Yoshikawa T., Yoshioka Y., Kamada H., Mukai Y., Nakagawa S., Tsutsumi Y., Tsunoda S.
  • Journal Title: Biomaterials 32(1) Pages: 162-169
  • Peer Reviewed
• [Journal Article] Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung. (2011)
  • Author(s): Yoshida Y., Yamashita T., Nagano K., Imai S., Nabeshi H., Yoshikawa T., Yoshioka Y., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 405(4) Pages: 610-614
  • Peer Reviewed
• [Journal Article] Development of a novel antibody proteomics system using a phase antibody library for efficient screening of tumor-related biomarker proteins. (2011)
  • Author(s): Imai S., Nagano K., Yoshida Y., Okamura T., Yamashita T., Abe Y., Yoshikawa T., Yoshioka Y., Kamada H., Mukai Y., Nakagawa S., Tsutsumi Y., Tsunoda S.
  • Journal Title: Biomaterials Volume: 32(1) Pages: 162-169
  • Peer Reviewed
• [Presentation] Proteomics-based analysis of non-alcoholic fatty liver disease (NAFLD) in mice (2010)
  • Author(s): Watanabe T., Yamashita T., Nagano K., Kanasaki S., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
  • Organizer: HUP02010 World Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-20
• [Presentation] プロテオミクスによる非アルコール性脂肪性肝疾患(NAFLD/NASH)のバイオマーカー探索 (2010)
  • Author(s): 渡邉貴信、長野一也、山下琢矢、岡村賢孝、金崎聡一郎、阿部康弘、吉川友章、吉岡靖雄、鎌田春彦、伊藤徳夫、小泉桂一、角田慎一、堤康央
  • Organizer: 日本薬学会130年会
  • Place of Presentation: 岡山
• [Presentation] Proteomics-based analysis of non-alcoholic fatty liver disease (NAFLD) in mice. (2010)
  • Author(s): Watanabe T., Yamashita T., Nagano K., Kanasaki S., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
  • Organizer: HUPO2010 World Congress
  • Place of Presentation: Sydney(Australia)