| Project/Area Number |
21F20708
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 28050:Nano/micro-systems-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
竹内 昌治 東京大学, 生産技術研究所, 教授 (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
LISI FABIO 東京大学, 生産技術研究所, 外国人特別研究員
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| Project Period (FY) |
2021-04-28 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2021: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Nanoparticles / hydrogel / controlled release / DNA / photothermal effect / microfluiddic / cell culture / epithelial cells |
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| Outline of Research at the Start |
This research project focuses of controlling the structural, biophysical and biochemical properties of commercial hydrogels via localised delivery of signalling molecules. These molecules can influence the cells embedded on the hydrogel or on the gel itself, tuning its mechanical properties. The delivery of these chemicals is triggered by using visible or near infrared light, a method that does not damage the cells and could potentially used in-vivo. The results of this project will correlate the dynamic changes in the hydrogel microenvironment to cell morphology and behaviour.
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| Outline of Annual Research Achievements |
The focus of my research was the development of various nanoparticle systems that could be introduced into commercial hydrogels to tune their properties. Together with a collaborator, I developed a method to coat large beads with functional polymers, a study that was published on Colloid and Interface Science Communications. Such a method required a precise control of the particles’ surface, and a second article that describes this surface reaction is currently under preparation. I then used this method to prepare gelatin-coated beads that are responsive to matrix metalloproteinase 9 (MMP-9), an enzyme that plays an important role in various diseases. The plan is to use these beads to develop a MMP-9 biosensor in FY22.
Another nanoparticle system that I developed was based on gold nanorods and DNA, for the targeted release of enzymes. Gold nanorods can very efficiently heated using a red laser, and the resulting heath is transferred to the surrounding environment. When double strands of DNA are immobilized on the rods’ surface, the heath causes DNA melting and release of one strand. I confirmed this process using fluorophore-labelled DNA, the next step will be using an enzyme-labelled DNA. Finally, I also developed a liposomes loaded with Ca2+ ions, which could release their cargo when the temperature is above 40 C. An increase of Ca2+ ions can cause physical changes in some hydrogels, for example an increase in stiffness of alginate gels. In order to selectively heath only the liposomes, small (4 nm) gold particles were embedded in their phospholipid bilayer.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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