SCN2A自閉症に関する膜電位イメージングによる細胞体樹状突起シグナリングの解析
| Project/Area Number |
21F20712
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Kuhn Bernd 沖縄科学技術大学院大学, 光学ニューロイメージングユニット, 教授 (90599557)
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| Co-Investigator(Kenkyū-buntansha) |
LI MELODY 沖縄科学技術大学院大学, 光学ニューロイメージングユニット, 外国人特別研究員
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| Project Period (FY) |
2021-09-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2022: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Scn2a/Nav1.2 / neurological disorders / In vivo calcium imaging |
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| Outline of Research at the Start |
Dr. Melody Li will establish a method to label neurons with the voltage-sensitive dye ANNINE-6plus. This technique will allow her to image voltage changes in the dendrites of pyramidal neurons with two-photon microscopy. After establishing this technique, Melody with study a specific type of autism spectrum disorder which is caused by a genetic mutations in the SCN2A gene. So far it is unknown how this SCN2A mutations alters brain function. We hope that this research will elucidate the mechanism of this disorder and open the door for finding a cure.
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| Outline of Annual Research Achievements |
Established a novel mouse model to study disease mechanisms underlying Scn2a neurodevelopmental disorders. For the first time, in vivo two-photo calcium imaging was performed in a Scn2a mouse model. We found that single neuron activities, as well as population co-activities were reduced in Scn2a insufficient mice during wakefulness. This may be one of the pathological mechanisms driving Scn2a neurodevelopmental disorders. Results was presented in the Japan neuroscience Society Annual Meeting and in the Okinawa Institute of Science and Technology (OIST) Internal Seminar.
A manuscript is in preparation and will be submitted to a peer reviewed journal.
Established collaboration opportunities between OIST and Florey Institute of Neuroscience and Mental Health, Australia.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(1 results)
