| Project/Area Number |
21F21412
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
岡本 章玄 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, グループリーダー (70710325)
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| Co-Investigator(Kenkyū-buntansha) |
NARADASU DIVYA 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 外国人特別研究員
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| Project Period (FY) |
2021-11-18 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2022: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2021: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | Porphyromonas gingivalis / Electron transport / Amono acid metabolism / Iron / implant corrosion / Extracellula / electron transfer / Membrane vesicles / Redox-repressor rex / Capnocytophaga ochracea / Streptococcus mutans |
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| Outline of Research at the Start |
To check the iron acquisition and export, PG will be tested for the release of iron during its EET by ferrozine assay, differential pulse voltammogram (DPV) analysis and spectroscopic iron assays, i.e., export if (Fe2+) via an unknown iron exporter.
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| Outline of Annual Research Achievements |
Porphyromonas gingivalis (PG) is a typical member of the dental plaque biofilm found on the tooth surface. PG is a keystone pathogen associated with chronic oral diseases and systemic inflammatory disorders such as cardiovascular, arthritic, and neurodegenerative diseases. This research aims to identify the critical mechanisms of EET coupled iron export in PG leading to inflammation and corrosion. PG can take up soluble Fe2+ and store it in oxidized Fe3+ form intracellularly to utilize in times of iron limitation. However, whilst essential to avoid toxicity due to excess iron, PG iron homeostasis and the export mechanism is not well understood. This study investigated and compared the extracellular elecctron transport (EET) capabilities of PG using various amino substrates. With a coulombic efficiency (CE) of 2.8%, P. gingivalis considerably increased current with histidine metabolism compared to other substrates, which is around 1000 times higher than the CE that P. gingivalis was previously reported to have for glucose oxidation (0.003%). During this research, important identification of Iron reduction by P. gingivalis was tested and identified the same, indicating the possible mechanism of EET coupled with Iron reduction by P. gingivalis. And iron has showed the impact on the growth of pathogen reflected in the electron transfer capability as well. Our findings demonstrate that amino acids, particularly histidine, which has an eight times greater current production rate than glucose, can serve as carbon sources for the asaccharolytic pathogen P. gingivalis.
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| Research Progress Status |
翌年度、交付申請を辞退するため、記入しない。
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| Strategy for Future Research Activity |
翌年度、交付申請を辞退するため、記入しない。
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