| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
Scanning probe microscopy and electrochemical devices were applied to characterize human mesenchymal stem cell (hMSC) differentiation and epithelial-to-mesenchymal transition (EMT) processes. The osteoblast differentiation process of hMSCs was quantitatively evaluated by electrochemical activity and morphological changes. In evaluating the epithelial-to-mesenchymal transition EMT process using SICM, we were able to establish a process to analyze shape changes and appearance rates such as ruffles. By introducing a probe microscope from the open-top of the multilayered microfluidic device, we succeeded to generate laminar flow and apply shear stress to vascular endothelial cells.
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