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Improvement of N use efficiency in photosynthesis and plant productivity via enhancement of the photosynthetic carbon metabolism from carbon fixation to triose phosphate production

Research Project

Project/Area Number 21H02084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionIwate University

Principal Investigator

Suzuki Yuji  岩手大学, 農学部, 教授 (80374974)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywords光合成 / カルビンサイクル / 遺伝子組換え / イネ / 炭酸同化
Outline of Research at the Start

本研究では、光合成炭酸同化を担うカルビンサイクルにおいて、Rubiscoによる炭酸固定からトリオースリン酸の生成までの一連の反応を強化することで、光合成能力およびバイオマス生育量の増大およびこれらの窒素利用効率の改良をねらう。このため、該当するカルビンサイクル酵素を増強したイネを交配し、作製したイネについて上記の解析を行う。

Outline of Final Research Achievements

In this study, N use efficiency in photosynthesis and plant productivity was tried to be improved in rice. For this purpose, the photosynthetic carbon metabolism from carbon fixation to triose phosphate production was enhanced. Firstly, the reactions downstream carbon fixation were investigated and the enzyme for metabolic enhancement was identified. Then, three enzymes, including one previously found to be effective, were simultaneously overexpressed. However, photosynthesis or its N use efficiency was scarcely affected. Possibly, simultaneous overexpression of two enzymes downstream carbon fixation was not enough for substantial enhancement in the photosynthetic carbon metabolism. It seems necessary to simultaneously enhance the ATP and NADPH supply, which are required in this pathway.

Academic Significance and Societal Importance of the Research Achievements

本研究の最終的な目的は果たせなかったが、今後の光合成炭酸同化系の強化ポイント特定に有用な情報を提示することができた。方法的には、光合成炭酸同化に関与する酵素の三重増強という世界的に類を見ない試みがなされ、本研究の独自性となっている。また、これまでの光合成に関する常識を覆す結果等も得られている。例えば、これまでは光合成にとって大過剰に存在するとされてきた酵素が、実際には大過剰ではなく光合成をある程度律速しうるものであった。以上から、本研究の成果は、光合成炭酸同化の研究において、世界的にみても貴重なものとなっている。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (10 results)

All 2023 2022 2021

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Behavior of Photosystems II and I Is Modulated Depending on N Partitioning to Rubisco in Mature Leaves Acclimated to Low N Levels and Senescent Leaves in Rice2023

    • Author(s)
      Yuji Suzuki, Kaho Ohsaki, Yuki Takahashi, Shinya Wada, Chikahiro Miyake, Amane Makino
    • Journal Title

      Plant & Cell Physiology

      Volume: 64 Issue: 1 Pages: 55-63

    • DOI

      10.1093/pcp/pcac139

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of simultaneous Rubisco, glyceraldehyde-3-phosphate dehydrogenase, and triosephosphate isomerase overexpression on photosynthesis in rice2023

    • Author(s)
      Yuji Suzuki, Mikuri Yamashita, Haruka Takada, Yuki Takegahara-Tamakawa, Chikahiro Miyake, Amane Makino、Miyake Chikahiro、Makino Amane
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: Published online Issue: 5-6 Pages: 1-8

    • DOI

      10.1080/00380768.2023.2255213

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice2022

    • Author(s)
      Suzuki Y, Konno Y, Takegahara-Tamakawa Y, Miyake C, Makino A
    • Journal Title

      Photosynthesis Research

      Volume: 153 Issue: 1-2 Pages: 83-91

    • DOI

      10.1007/s11120-022-00923-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of Chloroplast Triosephosphate Isomerase Marginally Improves Photosynthesis at Elevated CO2 Levels in Rice2022

    • Author(s)
      Suzuki Y, Shiina M, Takegahara-Tamakawa Y, Miyake C, Makino A
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 10 Pages: 1500-1509

    • DOI

      10.1093/pcp/pcac115

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of co-overproduction of Rubisco and chloroplast glyceraldehyde-3-phosphate dehydrogenase on photosynthesis in rice2021

    • Author(s)
      Suzuki Y*, Ishiyama K, Cho A, Takegahara-Tamakawa Y, Wada S, Miyake C and Makino A
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 67 Issue: 3 Pages: 1150-1162

    • DOI

      10.1080/00380768.2021.1915100

    • NAID

      210000158988

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] Rubisco・GAPDH・TPIの同時増強がイネの光合成に及ぼす影響2023

    • Author(s)
      鈴木雄二, 山下みくり, 高田遥日, 竹ケ原(玉川), 夕紀, 三宅親弘, 牧野 周
    • Organizer
      日本土壌肥料学会東北支部大会2023年度岩手大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] イネにおける外因性・内因性ストレスとP700 酸化:水ストレスと葉の老化の事例 から2023

    • Author(s)
      鈴木雄二
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 葉緑体型トリオースリン酸イソメラーゼの増強がイネの光合成と個体生育に及ぼす影響2022

    • Author(s)
      鈴木雄二、椎名美月、竹ケ原(玉川)夕紀、三宅親弘、牧野周
    • Organizer
      日本土壌肥料学会東北支部大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 葉緑体型トリオースリン酸イソメラーゼの抑制がイネの光合成に及ぼす影響2022

    • Author(s)
      鈴木 雄二,石山 敬貴,尹 棟敬,竹ケ原(玉川) 夕紀,近藤 依里,菅波 眞央,和田 慎也, 三宅 親弘,牧野 周
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Flavodiiron protein の導入はRubisco activase 過剰発現イネにおけるPSI 電子伝達 異常を回復させる2022

    • Author(s)
      菅波 眞央,今野 壮,丸橋 凌,高木 大輔,田副 雄士,和田 慎也,山本 宏,鹿内 利治,石田 宏幸, 鈴木 雄二,牧野 周
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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